Molecular-genetic characteristics of strain Escherichia coli serogroup O26 causing diarrheal diseases in children



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Abstract

Aim. To determine the serotype and virulence factors of the E. coli serogroup O26 isolated from children with diarrheal syndrome. Materials and methods. Fifty-three strains of E. coli O26 isolated in 2014-2016 from the stool of children with clinical manifestations of acute intestinal infection in Saint Petersburg were studied. Phenotypic (enzymatic and antigenic properties), molecular genetic [detection of virulence genes of enteropathogenic (EPEC - eae, bfp, hlyA), and enterohemorrhagic (EHEC - eae, stx1, stx2, ehxA), genes encoding O- and H-antigens (rfb and fliC), genes, defining phylogenetic group (chuA, yjaA и TspE)] methods were used. The phylogenetic group and the production Shiga toxins were determined. Results. All strains were identical to the antigen characteristics of serotype O26:H11 and phylogenetic group B1. Two pathogroups were created based on the set of virulence genes: a-EPEC (64.2%) and EHEC (35.8%). Strains EHEC produced Shiga-toxin 1, encoding gen stx1. No differences in enzymatic activities were found between the strains of E. coli О26:H11 for EPEC and EHEC strains. Conclusion. In the population of E. coli O26:H11, which caused acute intestinal infection in children in Saint Petersburg, more than 30% of the strains belonged to the highly virulent group EHEC. Molecular-genetic methods should be used for reliable detection of pathogens.

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About the authors

M A Makarova

Saint Petersburg Pasteur Institute

A V Dmitriev

Institute of Experimental Medicine

Z N Matveeva

Saint Petersburg Pasteur Institute

K A Kaftyreva

Saint Petersburg Pasteur Institute

References

  1. Кафтырева Л.А., Егорова С.А., Макарова М.А., и др. Характеристика биологических свойств E. coli О104:Н4 - возбудителя крупной пищевой вспышки, возникшей в Германии в мае 2011 г. // Клиническая лабораторная диагностика. - 2012. - № 1. - С. 44-47. [Kaftyreva LA, Egorova SA, Makarova MA, et al. The characteristics of biological properties of E. coli O104:H4 - the causative agent of large-scale alimentary ictus in Germany May 2001. Klin Lab Diagn. 2012;(1):44-47. (In Russ.)]
  2. Федеральная служба государственной статистики (Росстат). Здравоохранение в России - 2017. Статистический сборник. - М., 2017. [Federal’naya sluzhba gosudarstvennoy statistiki (Rosstat). Zdravookhranenie v Rossii - 2017. Statisticheskiy sbornik. Moscow; 2017. (In Russ.)]
  3. Bell BP, Griffin PM, Lozano P, et al. Predictors of hemolytic uremic syndrome in children during a large outbreak of Escherichia coli O157:H7 infections. Pediatrics. 1997;100(1):E12. doi: 10.1542/peds.100.1.e12.
  4. Bielaszewska M, Zhang W, Tarr PI, et al. Molecular profiling and phenotype analysis of Escherichia coli O26:H11 and O26:NM: secular and geographic consistency of enterohemorrhagic and enteropathogenic isolates. J Clin Microbiol. 2005;43(8):4225-4228. doi: 10.1128/JCM.43.8.4225-4228.2005.
  5. Онищенко Г.Г., Дятлов И.А, Светоч Э.А., и др. Молекулярно-генетическая характеристика шига-токсинпродуцирующих Escherichia coli, выделенных при вспышке пищевой инфекции в Санкт-Петербурге в 2013 году // Вестник Российской академии медицинских наук. - 2015. - Т. 70. - № 1. - С. 70-81. [Onishchenko GG, Dyatlov IA, Svetoch EA, et al. Molecular-genetic Characterization of Shiga-Toxin Producing Escherichia сoli Isolated During a Food-Borne Outbreak in Saint Petersburg in 2013. Annals of the Russian academy of medical sciences. 2015;70(1):70-81. (In Russ.)]. doi: 10.15690/vramn.v70i1.1234.
  6. Bielaszewska M, Mellmann A, Bletz S, et al. Enterohemorrhagic Escherichia coli O26:H11/H-: a new virulent clone emerges in Europe. Clin Infect Dis. 2013;56(10):1373-1381. doi: 10.1093/cid/cit055.
  7. Misselwitz J, Karch H, Bielazewska M, et al. Cluster of hemolytic-uremic syndrome caused by Shiga toxin-producing Escherichia coli O26:H11. Pediatr Infect Dis J. 2003;22(4):349-354. doi: 10.1097/01.inf.0000059338.38673.ae.
  8. Nataro JP, Kaper JB. Diarrheagenic Escherichia coli. Clin Microbiol Rev. 1998;11(1):142-201. doi: 10.1128/CMR.11.1.142.
  9. Kruger A, Lucchesi PM, Sanso AM, et al. Genetic characterization of Shiga toxin-producing Escherichia coli O26:H11 strains isolated from animal, food, and clinical samples. Front Cell Infect Microbiol. 2015;5:74. doi: 10.3389/fcimb.2015.00074.
  10. Vygen-Bonnet S, Rosner B, Wilking H, et al. Ongoing haemolytic uraemic syndrome (HUS) outbreak caused by sorbitol-fermenting (SF) Shiga toxin-producing Escherichia coli (STEC) O157, Germany, December 2016 to May 2017. Euro Surveill. 2017;22(21). doi: 10.2807/1560-7917.ES.2017.22.21.30541.
  11. Bonardi S, Alpigiani I, Tozzoli R, et al. Shiga toxin-producing Escherichia coli O157, O26 and O111 in cattle faeces and hides in Italy. Vet Rec Open. 2015;2(1):e000061. doi: 10.1136/vetreco-2014-000061.
  12. Brooks JT, Sowers EG, Wells JG, et al. Non-O157 Shiga toxin-producing Escherichia coli infections in the United States, 1983-2002. J Infect Dis. 2005;192(8):1422-1429. doi: 10.1086/466536.
  13. Durso LM, Bono JL, Keen JE. Molecular serotyping of Escherichia coli O26:H11. Appl Environ Microbiol. 2005;71(8):4941-4944. doi: 10.1128/AEM.71.8.4941-4944.2005.
  14. Guion CE, Ochoa TJ, Walker CM, et al. Detection of diarrheagenic Escherichia coli by use of melting-curve analysis and real-time multiplex PCR. J Clin Microbiol. 2008;46(5):1752-1757. doi: 10.1128/JCM.02341-07.
  15. Tobias J, Vutukuru SR. Simple and rapid multiplex PCR for identification of the main human diarrheagenic Escherichia coli. Microbiol Res. 2012;167(9):564-570. doi: 10.1016/j.micres.2011.11.006.
  16. El-Rami FE, Rahal E, Sleiman FT, Abdelnoor AM. Identification of virulence genes among antibacterial-resistant Escherichia coli. Adv Stud Biol. 2012;4(8):385-396.
  17. Slanec T, Fruth A, Creuzburg K, Schmidt H. Molecular analysis of virulence profiles and Shiga toxin genes in food-borne Shiga toxin-producing Escherichia coli. Appl Environ Microbiol. 2009;75(19):6187-6197. doi: 10.1128/AEM.00874-09.
  18. Clermont O, Bonacorsi S, Bingen E. Rapid and simple determination of the Escherichia coli phylogenetic group. Appl Environ Microbiol. 2000;66(10):4555-4558. doi: 10.1128/AEM.66.10.4555-4558.2000.
  19. Afset JE, Bergh K, Bevanger L. High prevalence of atypical enteropathogenic Escherichia coli (EPEC) in Norwegian children with diarrhoea. J Med Microbiol. 2003;52(Pt 11):1015-1019. doi: 10.1099/jmm.0.05287-0.
  20. Соколова Е.Д., Галтаева А.М., Замурин О.Ю., и др. Полимеразно-цепная реакция в диагностике острых кишечных инфекций в детском инфекционном стационаре: возможности и проблемы // Инфекция и иммунитет. - 2016. - Т. 6. - № 3. - С. 225-231. [Sokolova ED, Galtaeva AM, Zamurin OY, et al. Acute enteric infections polymerase chain reaction assay in pediatric practice: opportunities and challenges. Russian journal of infection and immunity. 2016;6(3):225-231. (In Russ.)]. doi: 10.15789/2220-7619-2016-3-225-231.

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Copyright (c) 2018 Makarova M.A., Dmitriev A.V., Matveeva Z.N., Kaftyreva K.A.

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