Revealing of immunopositive beta-adrenergic structures, using polyclonal antibodies to ADRB2 (GLN247-SER262) in the lungs of rats

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Abstract


The aim of this research was to identify ADRB2-immunopositive structures in the lungs of intact rats.

Materials and methods. The material for the study were the lungs of adult male rats (n = 6) with a verified absence of signs of inflammation. The obtained material was fixed in zinc-ethanol-formaldehyde by a standard procedure. Identification of ADRB2-immunopositive structures was performed using the immunohistochemistry method.

Results. The study revealed the intensive and selective diffuse staining of the apical part of the bronchial airway epithelium, reflecting the preferential distribution of ADRB2-immunopositive structures in the large and medium bronchi and partially in the terminal bronchioles of the intrapulmonary airways. The ADRB2-immunopositive structures were characterized by clear boundaries, with a slightly vague halo, and having a rounded or strongly elongated oval shape. The size of the identified structures varied depending on the caliber of the bronchi, in which they were localized.

Conclusion. This research identified specific ADRB2-immunopositive structures localized in the epithelium of the respiratory tract. In addition, it was demonstrated that polyclonal antibodies used in the research selectively detect ADRB2-immunopositive structures only in the bronchial epithelium, without engaging in immunohistochemically interaction with beta-2 smooth muscle adrenergic receptors, which is apparently due to a higher concentration of the immunoreactive product in epithelial cells compared to smooth muscles.


Marina A. Syrczova

Institute of Experimental Medicine

Author for correspondence.
Email: iemmorphol@yandex.ru
ORCID iD: 0000-0002-6974-4062
SPIN-code: 7826-8133

Russian Federation, Saint Petersburg

research scientist, Laboratory of Functional Morphology of Central and Peripheral Nervous System, Morphology Department

Dmitriy E. Korzhevskii

Institute of Experimental Medicine

Email: iemmorphol@yandex.ru
ORCID iD: 0000-0002-2456-8165
SPIN-code: 3252-3029

Russian Federation, Saint Petersburg

Doctor of Medical Sciences, Laboratory of Functional Morphology of Central and Peripheral Nervous System, Morphology Department

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Supplementary files

Supplementary Files Action
1. Fig. 1. ADRB2-immunopositive structures in the middle bronchus. Immunohistochemical reaction to ADRB2 (hematoxylin staining), scale bar 0.02 mm (a); ADRB2-immunopositive structures in the small bronchus. Immunohistochemical reaction to ADRB2 (hematoxylin staining), scale bar 0.05 mm (b) View (290KB) Indexing metadata

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Copyright (c) 2019 Syrczova M.A., Korzhevskii D.E.

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