Revealing of immunopositive beta-adrenergic structures, using polyclonal antibodies to ADRB2 (GLN247-SER262) in the lungs of rats

Cover Page
Open Access Open Access
Restricted Access Subscription Access


The aim of this research was to identify ADRB2-immunopositive structures in the lungs of intact rats.

Materials and methods. The material for the study were the lungs of adult male rats (n = 6) with a verified absence of signs of inflammation. The obtained material was fixed in zinc-ethanol-formaldehyde by a standard procedure. Identification of ADRB2-immunopositive structures was performed using the immunohistochemistry method.

Results. The study revealed the intensive and selective diffuse staining of the apical part of the bronchial airway epithelium, reflecting the preferential distribution of ADRB2-immunopositive structures in the large and medium bronchi and partially in the terminal bronchioles of the intrapulmonary airways. The ADRB2-immunopositive structures were characterized by clear boundaries, with a slightly vague halo, and having a rounded or strongly elongated oval shape. The size of the identified structures varied depending on the caliber of the bronchi, in which they were localized.

Conclusion. This research identified specific ADRB2-immunopositive structures localized in the epithelium of the respiratory tract. In addition, it was demonstrated that polyclonal antibodies used in the research selectively detect ADRB2-immunopositive structures only in the bronchial epithelium, without engaging in immunohistochemically interaction with beta-2 smooth muscle adrenergic receptors, which is apparently due to a higher concentration of the immunoreactive product in epithelial cells compared to smooth muscles.

Marina A. Syrczova

Institute of Experimental Medicine

Author for correspondence.
ORCID iD: 0000-0002-6974-4062
SPIN-code: 7826-8133

Russian Federation, Saint Petersburg

research scientist, Laboratory of Functional Morphology of Central and Peripheral Nervous System, Morphology Department

Dmitriy E. Korzhevskii

Institute of Experimental Medicine

ORCID iD: 0000-0002-2456-8165
SPIN-code: 3252-3029

Russian Federation, Saint Petersburg

Doctor of Medical Sciences, Laboratory of Functional Morphology of Central and Peripheral Nervous System, Morphology Department

  1. Nasser Y, Ho W, Sharkey KA. Distribution of adrenergic receptors in the enteric nervous system of the guinea pig, mouse, and rat. J Comp Neurol. 2006;495(5):529-553.
  2. Amrani Y, Bradding P. Beta2-adrenoceptor function in asthma. Adv Immunol. 2017;136:1-28.
  3. Chowdhury BA, Dal Pan G. The FDA and safe use of long-acting beta-agonists in the treatment of asthma. N Engl J Med. 2010;362(13):1169-1171.
  4. Nguyen LP, Al-Sawalha NA, Parra S, et al. beta2-Adrenoceptor signaling in airway epithelial cells promotes eosinophilic inflammation, mucous metaplasia, and airway contractility. Proc Natl Acad Sci U S A. 2017;114(43):E9163-E9171.
  5. Nguyen LP, Lin R, Parra S, et al. Beta2-adrenoceptor signaling is required for the development of an asthma phenotype in a murine model. Proc Natl Acad Sci U S A. 2009;106(7):2435-2440.
  6. Трофимов В.И., Миронова Ж.А., Дубина М.В. Генетический профиль терапевтически резистентной бронхиальной астмы // Медицинский академический журнал. – 2013. – Т. 13. – № 2. – С. 44–50. [Trofimov VI, Mironova ZA, Dubina MV. Genetic profile of therapy-resistant asthma. Med Acad J. 2013;13(2);44-45. (In Russ.)]
  7. Ворончихин П.А., Сырцова М.А., Талантов С.В., и др. Влияние метопролола и бисопролола на течение экспериментальной бронхиальной астмы // Биомедицина. – 2013. – № 2. – С. 42–51. [Voronchikhin PA, Syrtsova MA, Talantov SV, et al. Effect of metoprolol and bisoprolol on the course of experimental bronchial asthma. Biomeditsina. 2013;(2):42-51. (In Russ.)]
  8. Lelliott A, Nikkar-Esfahani A, Offer J, et al. The role of extracellular-signal regulate kinase (ERK) in the regulation of airway tone in porcine isolated peripheral bronchioles. Eur J Pharmacol. 2012;674(2-3):407-414.
  9. Grayson MH, Feldman S, Prince BT, et al. Advances in asthma in 2017: Mechanisms, biologics, and genetics. J Allergy Clin Immunol. 2018;142(5):1423-1436.
  10. Yarova PL, Stewart AL, Sathish V, et al. Calcium-sensing receptor antagonists abrogate airway hyperresponsiveness and inflammation in allergic asthma. Sci Transl Med. 2015;7(284):284ra260.
  11. Thanawala VJ, Forkuo GS, Al-Sawalha N, et al. beta2-Adrenoceptor agonists are required for development of the asthma phenotype in a murine model. Am J Respir Cell Mol Biol. 2013;48(2):220-229.
  12. Koryakina YA, Fowler TW, Jones SM, et al. Characterization of a panel of six beta2-adrenergic receptor antibodies by indirect immunofluorescence microscopy. Respir Res. 2008;9:32.

Supplementary files

Supplementary Files Action
1. Fig. 1. ADRB2-immunopositive structures in the middle bronchus. Immunohistochemical reaction to ADRB2 (hematoxylin staining), scale bar 0.02 mm (a); ADRB2-immunopositive structures in the small bronchus. Immunohistochemical reaction to ADRB2 (hematoxylin staining), scale bar 0.05 mm (b) View (290KB) Indexing metadata


Abstract - 37

PDF (Russian) - 0




  • There are currently no refbacks.

Copyright (c) 2019 Syrczova M.A., Korzhevskii D.E.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.