The influence of acizol into effects of picrotoxin, injected in rat’s neostriatum

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Abstract

The aim of the article. The article is devoted to investigation of of zinc donator acizol influence to rat’s behavior, broken by intrastriatal injection of GABA-A receptor antagonist picrotoxin.

Materials and methods. Adult male Wistar rats with avoidance conditioning reflexes in “shuttle box” and free locomotor activity in “open field” were used. Daily microinjection of picrotoxin (2 mcg/1 mcl) bilateral into rostral neostriatum in term of 15 days were made. Zinc donator acizol was injected intraperitoneal (24 mg/kg).

Results. Steady losses of avoidance conditioning and choreo-mioclonic hyperkinesis of limbs and body, similar with human Huntington’s chorea by picrotoxin were produced. Acizol is contribute to restore avoidance conditioning and to prevent the development of hyperkinesis or essentially extend latency and lover duration of it.

Conclusion. With the early data obtained, there is reason to propose, that acizol, to increasing the zinc content in the body, especially in the brain, is recover damaged cognitive function and to prevent the picrotoxin-induced hyperkinesis. Acizol should be proposed as perspective drug in extrapyramidal hyperkinetic deviation treatment in human.

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About the authors

Аndrey F. Yakimovskii

Pavlov First Saint Petersburg State Medical University; Pavlov Institute of Physiology, Russian Academy of Science

Author for correspondence.
Email: jakim2010@gmail.com

MD, Professor, Chief of Laboratory of Normal and Pathological Locomotor Behaviour; Senior Research Scientist in Laboratory of Physiology Higher Nervous Activity

Russian Federation, Saint Petersburg

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Supplementary files

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2. Fig. 1. Conditioned avoidance performance in a shuttle box (mean values) in rats with intrastriatal injection of 1 mcl physiological saline (a), 2 μg picrotoxin (b) and 2 μg picrotoxin with 24 mg/kg of acizol simultaneously (с). The abscissa shows experimental days: I —baseline before injections, II — days of treatment and III — withdrawal days. Ordinate: ratio between the number of conditioned responses and total number of presented conditioned stimuli; ** p = 0.01 compared to the control

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3. Fig. 2. Latency (A) and duration (B) of hyperkinesis, produced by 2 μg picrotoxin intrastriatal microinjection in rat’s with picrotoxin alone (a) and with 2 μg picrotoxin microinjection and with intraperitoneal injection of 24 mg/kg acizol (b). Ordinate: time in minutes; ** p = 0.01 compared to rat’s with picrotoxin microinjection alone

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