The influence of acizol into effects of picrotoxin, injected in rat’s neostriatum

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Abstract

The aim of the article. The article is devoted to investigation of of zinc donator acizol influence to rat’s behavior, broken by intrastriatal injection of GABA-A receptor antagonist picrotoxin.

Materials and methods. Adult male Wistar rats with avoidance conditioning reflexes in “shuttle box” and free locomotor activity in “open field” were used. Daily microinjection of picrotoxin (2 mcg/1 mcl) bilateral into rostral neostriatum in term of 15 days were made. Zinc donator acizol was injected intraperitoneal (24 mg/kg).

Results. Steady losses of avoidance conditioning and choreo-mioclonic hyperkinesis of limbs and body, similar with human Huntington’s chorea by picrotoxin were produced. Acizol is contribute to restore avoidance conditioning and to prevent the development of hyperkinesis or essentially extend latency and lover duration of it.

Conclusion. With the early data obtained, there is reason to propose, that acizol, to increasing the zinc content in the body, especially in the brain, is recover damaged cognitive function and to prevent the picrotoxin-induced hyperkinesis. Acizol should be proposed as perspective drug in extrapyramidal hyperkinetic deviation treatment in human.

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About the authors

Аndrey F. Yakimovskii

Pavlov First Saint Petersburg State Medical University; Pavlov Institute of Physiology, Russian Academy of Science

Author for correspondence.
Email: jakim2010@gmail.com

MD, Professor, Chief of Laboratory of Normal and Pathological Locomotor Behaviour; Senior Research Scientist in Laboratory of Physiology Higher Nervous Activity

Russian Federation, Saint Petersburg

References

  1. Селиверстов Ю.А., Клюшников С.А., Иллариошкин С.Н. Лечение гиперкинезов при болезни Гентингтона // Me-dica mente. Неврология. – 2018. – Т. 4. – № 1. – С. 20–24. [Seliverstov YuА, Klyushnikov SA, Illarioshkin SN. Treatment of hyperkinesis in Huntington’s disease. Medica Mente. Neurology. 1918;4(1):20-24. (In Russ.)]
  2. Якимовский А.Ф., Варшавская В.М. Этиопатогенез хореи Гентингтона: итоги и перспективы экспериментального моделирования // Медицинский aкадемический журнал. – 2006. – Т. 6. – № 2. – С. 28–40. [Yakimovskii AF, Varshavskaya VM. Ethiopatogenesis of Huntington’s disease: results and perspectives of experimental modeling. Medical academic journal. 2006;6(2):28-40. (In Russ.)]
  3. Якимовский А.Ф., Крыжановская С.Ю. Влияние внутристриарных введений ацетата цинка на нормальное и патологическое двигательное поведение крыс // Медицинский академический журнал. – 2015. – Т. 15. – № 2. – С. 50–54. [Yakimovskii AF, Kryzhanovskaya SYu. The effect of intrastriatal zinc acetate injections on normal and pathological locomotor behavior in rats. Medical academic journal. 2015;15(2):50-54. (In Russ.)]
  4. Баринов В.А., Алексанин С.С., Радионов И.А., Шантырь И.И. Ацизол в комплексе мер защиты от токсических продуктов горения и лечения пострадавших // Медико-биологические и социально-психологические проблемы безопасности в чрезвычайных ситуациях. – 2011. – Т. 5. – № 1. – С. 14–19. [Barinov VA, Aleksanin SS, Radionov IA, Shantyr II. Acyzol within the package of measures for protection from toxic burning products and treatment of injured. Medico-biological and socio-psychological рroblems of safety in emergency situations. 2011;5(1):14-19. (In Russ.)]
  5. Якимовский А.Ф., Шантырь И.И., Власенко М.А., и др. Влияние ацизола на содержание биоэлементов в плазме крови, паренхиматозных органах и головном мозге крыс // Биомедицинская химия. – 2018. – Т. 64. – № 2. – С. 183–187. [Yakimovskii AF, Shantyr II, Vlasenko MA, et al. The influence of acyzolum to bioelements content in rat’s plasma, parenchymal organs and brain. Biomedical chemistry. 2018;64(2):183-187. (In Russ.)]. https://doi.org/10.18097/PBMC20186402183.
  6. Якимовский А.Ф., Шантырь И.И., Власенко М.А., Яковлева М.В. Влияние ацизола на содержание цинка в плазме крови и головном мозге крыс // Бюллетень экспериментальной биологии и медицины. – 2016. – T. 162. – № 9. – C. 268–270. [Yakimovskii AF, Shantyr II, Vlasenko MA, Yakovleva MV. The influence of acizol to zinc blood plasma and brain tissue in rats. Bulletin of experimental biology and medicine. 2016;162(9):268-270. (In Russ.)]
  7. Якимовский А.Ф. Нейробиология цинка // Успехи современной биологии. – 2019. – Т. 139. – № 3. – С. 267–279. [Yakimovskii AF. Neurobiology of zinc. Advances in current biology. 2019;139(3):267-279. (In Russ.). https://doi.org/10.1134/S0042132419030104.
  8. Smart TG, Hosie AM, Miller PS. Zn2+ ions: modulators of excitatory and inhibitory synaptic activity. Neuroscientist. 2004;10(5):432-442. https://doi.org/10.1177/ 1073858404263463.
  9. Kay AR, Toth K. Is zinc a modulator? Science signaling. 2008;1(19):1-7. https://stke.sciencemag.org/cgi/content/full/ sigtrans;1/19/re3.
  10. Mabrouk OS, Mela F, Calcagno M, et al. GluN2A and GluN2B NMDA receptor subunits differentially modulate striatal output pathways and contribute to levodopa-induced abnormal involuntary movements in dyskinetic rats. ACS Chemical Neuroscience. 2013;4(5):808-816. https://doi.org/10.1021/cn400016d.
  11. Frederickson ChJ, Suh SW, Silva D, et al. Importance of zinc in the central nervous system: the zinc-containing neuron. J Nutrition. 2000;130(5):1471S-1483S. https://doi.org/10.1093/jn/130.5.1471S.

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2. Fig. 1. Conditioned avoidance performance in a shuttle box (mean values) in rats with intrastriatal injection of 1 mcl physiological saline (a), 2 μg picrotoxin (b) and 2 μg picrotoxin with 24 mg/kg of acizol simultaneously (с). The abscissa shows experimental days: I —baseline before injections, II — days of treatment and III — withdrawal days. Ordinate: ratio between the number of conditioned responses and total number of presented conditioned stimuli; ** p = 0.01 compared to the control

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3. Fig. 2. Latency (A) and duration (B) of hyperkinesis, produced by 2 μg picrotoxin intrastriatal microinjection in rat’s with picrotoxin alone (a) and with 2 μg picrotoxin microinjection and with intraperitoneal injection of 24 mg/kg acizol (b). Ordinate: time in minutes; ** p = 0.01 compared to rat’s with picrotoxin microinjection alone

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