THE INTERACTION OF ARENICIN-1 WITH C3B COMPLEMENT PROTEIN

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Abstract

The complement system and antimicrobial peptides (AMPs) are known to be vital humoral factors of innate immunity. Earlier we showed the double-sided influence of arenicin-1 (Ar-1), the AMP from a sea polychaeta Arenicola marina, on the complement activation. In this work we studied the binding of Ar-1 to C3b protein, the fragment of the central complement component C3, using surface plasmon resonance. We also performed molecular docking and molecular dynamics of interaction between C3b fragment - C3c - and Ar-1. All these data showed that the influence of Ar-1 on complement activation might be realized through the interaction with C3b, the most important component for complement activation. Ar-1 may be used for the design of new complement regulators for treating complement-related diseases.

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E S Umnyakova

Institute of Experimental Medicine, Saint Petersburg

I A Krenev

Saint Petersburg State University

S V Legkovoy

Saint Petersburg State University

A V Sokolov

Institute of Experimental Medicine, Saint Petersburg

O N Rogacheva

Saint Petersburg State University

T V Ovchinnikova

M.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow

V N Kokryakov

Institute of Experimental Medicine, Saint Petersburg; Saint Petersburg State University

M N Berlov

Institute of Experimental Medicine, Saint Petersburg

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  7. Umnyakova ES, Gorbunov NP, Zhakhov AV, et al. Modulation of human complement system by antimicrobial peptide arenicin-1 from Arenicola marina. Marine Drugs. 2018;16:e480.

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Copyright (c) 2019 Umnyakova E.S., Krenev I.A., Legkovoy S.V., Sokolov A.V., Rogacheva O.N., Ovchinnikova T.V., Kokryakov V.N., Berlov M.N.

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