Lung Macrophages’ Reaction to Parenteral Administration of Heterocyclic Spiro-Fused Compounds in Rats

Background. Organ macrophages are the main part of mononuclear phagocytic system, which forms innate immunity. Nevertheless, there is not a well-known stereotypy of some of them reaction to xenobiotics.

Aim is the assessment in rats of the lung macrophages’ reaction to parenteral administration of heterocyclic spiro-fused compounds that are matrix for new antineoplastic agents.

Materials and methods. Wistar male rats (n=36) were divided into 3 groups where was a one-time intraperitoneal administration of spiro-fused Oxindole and Barbiturate or Normal saline (as a control). Then we euthanized 4 animals from each group in 2, 4, 8 weeks after administration and microscoped their lungs. We used a CD68-antibody for macrophages visualization.

Results. We present a reactivity of all macrophages’ types but there is not a stereotypy. The number of alveolar macrophages increases on early stages of the experiment whereas the number of interstitial macrophages decreases to the end of experiment.

Conclusions. All types of macrophages are sensitive to one-tine administration of spiro-fused Oxindole and Barbiturate. Both of these substances are able to penetrate throw the air-blood barrier and into the pulmonary stroma but induce different patterns of reactivity.