The multiple parallel analysis of gene expression as tool for diagnostic of renal cell carcinoma and prostate cancer

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Abstract

Many genes and signaling pathways are known to be involved in cancer initiation and progression. The molecular understanding of these processes is important for effective diagnostic and successful treatment of tumor diseases. The multiple parallel analysis of gene expression (cDNA array) is a powerful tool for simultaneous monitoring of the expression of thousand genes and identifying among them the ones with relevance to tumor. To discover the potential diagnostic and prognostic markers we have performed the expression profiling of more than 200 genes (protein-kinases and phosphatases) for tumor and normal tissues of patients with prostate cancer and renal cell carcinoma. The DUSP9 gene has showen the differential pattern of expression in normal and cancer renal tissues. We have used the semiquantitative RT-PCR to verify date obtained by cDNA array. We analyzed the expression of DUSP9 in 26 paired samples (cancer and adjacent normal kidney tissue). The analysis has confirmed the down-regulation of DUSP9 in tumor tissues The gene inactivation was revealed early in cancer development. DUSP9 codes the dual specificity protein kinase phosphatase 4 (MKP-4). The biological role of MKP-4 and loss of its expression in renal cancer could indicate a tumor suppressor function of this protein. Semiquantitative RT-PCR of other gene, ADFP also has confirmed cDNA array analysis date. The up-regulation was seen in 68% of renal cell carcinomas Thus the genes DUSP9 and ADFP, identified through the cDNA array may be potential candidates for renal cell cancer diagnostic and therapy.

About the authors

A. M. Granov

Central Research Institute of Roentgenology and Radiology

Author for correspondence.
Email: shabanov@mail.rcom.ru

Академик РАМН

Russian Federation, St. Petersburg

E. I. Yakubovich

Central Research Institute of Roentgenology and Radiology

Email: shabanov@mail.rcom.ru
Russian Federation, St. Petersburg

V. I. Evtushenko

Central Research Institute of Roentgenology and Radiology

Email: shabanov@mail.rcom.ru
Russian Federation, St. Petersburg

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