Email this article Low density lipoprotein subfraction with high negative charge
- Authors: Denisenko A.D.1, Silina I.A.1, Dizhe E.B.1, Vinogradov A.G.1, Klueva N.N.1
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Affiliations:
- Institute of Experimental Medicine
- Issue: Vol 1, No 2 (2001)
- Pages: 30-35
- Section: Basis medicine
- Published: 16.10.2025
- URL: https://journals.eco-vector.com/MAJ/article/view/693487
- DOI: https://doi.org/10.17816/MAJ693487
- ID: 693487
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Abstract
A minor electronegative low density lipoprotein subfraction (LDL-) was isolated from human plasma by combination of ultracentrifugation and anionexchange chromatography. LDL represents 5-15% of total LDL. Compared with the major LDL subfraction, designated LDL*, LDL: revealed increased contents of sialic acids. No difference was found in lipid and apoprotein composition between LDL* and LDL. The lipid peroxide concentration evaluated as conjugated dienes content in LDL- was slightly lower, than in LDL*, but there was no difference in susceptibility of LDL* and LDL- to copper-initiated oxidation. LDL' as well as LDL* were taken up by mouse peritoneal macrophages with the same low rate, but uptake of LDL by human lung fibroblasts was 30 % lower as compared to LDL*.Thus, the minor subfraction of LDL with elevated content of sialic acids and lowered affinity to LDL receptor is present in human plasma. It seems reasonable to suppose that elevated negative charge of LDL is provided by high content of sialic acids.
About the authors
A. D. Denisenko
Institute of Experimental Medicine
Author for correspondence.
Email: medaj@eco-vector.com
Russian Federation, St. Petersburg, 197376, 12 Acad. Pavlov St.
I. A. Silina
Institute of Experimental Medicine
Email: medaj@eco-vector.com
Russian Federation, St. Petersburg, 197376, 12 Acad. Pavlov St.
E. B. Dizhe
Institute of Experimental Medicine
Email: medaj@eco-vector.com
Russian Federation, St. Petersburg, 197376, 12 Acad. Pavlov St.
A. G. Vinogradov
Institute of Experimental Medicine
Email: medaj@eco-vector.com
Russian Federation, St. Petersburg, 197376, 12 Acad. Pavlov St.
N. N. Klueva
Institute of Experimental Medicine
Email: medaj@eco-vector.com
Russian Federation, St. Petersburg, 197376, 12 Acad. Pavlov St.
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СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).