Effect of amino acids and antibodies to nerve growth factor low-affinity receptors on the lymphoid tissue culture

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Abstract

The effect of L-amino acids in concentration 0.05 ng/ml was investigated in organotypic tissue culture of spleen in rats at age 3 months old. The high molecular and low hydrophobic amino acids - asparagine, lysine and glutamine acid are active, inducing more intensive growth zone in explants, as compared to control at the presence of the antibodies to nerve growth factor (NGF) low-affinity receptors (NGFRp75), being the apop­tosis inductors. In the contrast another group of low molecular and high hydrophobic amino acids which was active, inducing a less intensive growth zone and more p53 expression inhibition, produced at NGFRp75 presence the delay of the inhibition effect. These amino acids can mediate the apoptosis-induced effect by means of NGFRp75. The data obtained can be used by the synthesis of the novel bioregulator peptides.

About the authors

N. I. Chalisova

I. P. Pavlov Institute of Physiology of the Russian Academy Sciences

Author for correspondence.
Email: shabanov@mail.rcom.ru
Russian Federation, St. Petersburg

A. N. Zakutskiy

St. Petersburg Institute of Bioregulation and Gerontology of the North-Western Branch of the Russian Academy of Medical Sciences

Email: shabanov@mail.rcom.ru
Russian Federation, St. Petersburg

A. I. Aniskina

St. Petersburg Institute of Bioregulation and Gerontology of the North-Western Branch of the Russian Academy of Medical Sciences

Email: shabanov@mail.rcom.ru
Russian Federation, St. Petersburg

V. О. Polyakova

St. Petersburg Institute of Bioregulation and Gerontology of the North-Western Branch of the Russian Academy of Medical Sciences

Email: shabanov@mail.rcom.ru
Russian Federation, St. Petersburg

S. V. Filippov

St. Petersburg Institute of Bioregulation and Gerontology of the North-Western Branch of the Russian Academy of Medical Sciences

Email: shabanov@mail.rcom.ru
Russian Federation, St. Petersburg

P. N. Zezyulin

St. Petersburg Institute of Bioregulation and Gerontology of the North-Western Branch of the Russian Academy of Medical Sciences

Email: shabanov@mail.rcom.ru
Russian Federation, St. Petersburg

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