Changes in immunoreactivity of orexin-A-positive neurons after restraint stress and cold stress applications


As it is known influence of any stressor factors leads to considerable changes of immune system activity that affects current of infection process. Change of the expression level of preproorexin gene has been studied after restraint and cold-restraint stress application. Also selective alteration of immunereactivity of orexin-containing neurons is established after these kinds of stress application. Orexin-containing neurons are located mostly in structures on brain slices of 28,29,31 levels, and have different immunoreactivity, that testifies a functional heterogeneity of population of orexin-containing neurons in hypothalamus. The revealed changes of reactions in the central nervous system during stress-induced dysfunction of immune system are important for considering in clinical practice in the people with infection process who have transferred strong psychoemotional stress.

Full Text

Restricted Access

About the authors

K Z Shainidze

Research Institute of Experimental Medicine of the RAMS, St. Petersburg

N S Novikova

Research Institute of Experimental Medicine of the RAMS, St. Petersburg

G M Aleshina

Research Institute of Experimental Medicine of the RAMS, St. Petersburg

J A Darinsky

Gertsen State Pedagogical University, St. Petersburg

A P Synchikova

Research Institute of Experimental Medicine of the RAMS, St. Petersburg

E A Korneva

Research Institute of Experimental Medicine of the RAMS, St. Petersburg


  1. Корнева Е. А., ГПхинек Э. К. Гормоны и иммунная система. Л.: Наука, 1988. 251 с.
  2. Bartlett J. A., Demetrikopoulos М. К., Schleifer S. J. Phagocytosis and Killing of Staphylococcus aureus: Effects of Stress and Depression in Children // Clinical and diagnostic laboratory immunology. May 1997. Vol. 4. №3. P. 362-366.
  3. Becskei C, Hernadfalvy N, Arsenijevic D. et al. Inhibitory effects of LPS on hypothalamic nuclei involved in the control of food intake // Elsevier Ltd. 2007.
  4. Coppinge T. R., Minton J. E., Reddy P. G. Repeated restraint and isolation stress in lambs increases pituitaryadrenal secretions and reduces cell-mediated immunity. Kansas State University, Manhattan, 66506-0201 // J. Anim. Sci. 1991. Vol. 69. P. 2808-2814.
  5. Denes A., Boldogkoi Z., Uhereczky G. et al. Central automatic control of the bone marrow: multisynaptic tract trasing by recombinant pseudorabies virus // Neurosci. 2005. Vol. 134. №3. P. 947-963.
  6. Denes A., Boldogkoi Z., Uhereczy G. et al. Characterization of the central nervous system innervations of the rat spleen using viral transneuronal tracing // J. Сотр. Neurol. 2001. Vol. 439. P. 1-18.
  7. Estabrooke I. V, McCarthy M. Т., Ко E. et al. Fos expression in orexin neurons varies with behavioral state //J. Neurosci. 2001. Vol. 21. P. 1656-1662.
  8. Fleshner M., Nguyen Т. K, Cotter C. S, Watkins L. R. Acute stressor exposure both suppresses acquired immunity and potentiates innate immunity //Am. J. Physiol. Regulatory Integrative Сотр. Physiol. 2002. Vol. 282. P. R1680-R1686.
  9. Friedman E. M., Lawrence D. A. Environmental Stress Mediates Changes in Neuroimmunolog-ical Interactions // Toxicological sciences. 2002. Vol. 67. P. 4-10.
  10. Harbuz M. Neuroendocrine function and chronic inflammatory stress // Exp. Physiol. 2002. Vol. 87. №5. P. 519-525.
  11. Ida Т., Nakahara K., Murakami T. et al. Possible involvement of orexin in the stress reaction in rats // Biochem. Byophys. Res. Commun. 2000. Vol. 270. P. 318323.
  12. Jaszberenyi M., Bujdoso and Telegdy G. The role of neuropeptide Y in orexin-induced hypothalamic-pitu-itaryadrenal activation // J. Neuroendocrinol. 2001. Vol. 13. P. 438^141.
  13. Korneva E. A., Abdullaeva Z. A., Klimenko V. M., Nevidimov M. G. Immune response and changes in electrical activity of the brain // Peripheral signaling of the brain: role in neural-immune interaction and learning and memory: Program and proceeding. Irvine, USA. 1989. Vol. 6. P. 37.
  14. Kurose Т., Ueta Y, Yamamoto Y. et al. Effects of restricted feeding on the activity of hypothalamic orexin (OX)-A containing neurons and OX2 receptor mRNA level in the paraventricular nucleus of rats // Regul. Pept. 2002. Vol. 104. P. 145-151.
  15. Monda M., Viggiano A., Mondola P., de Luca V. Inhibition of prostaglandin synthesis reduces hyperthermic reactions induced by hypocretin-1/orexin A // Brain Res. 2001. Vol. 909. P. 68-74.
  16. Moriguchi Т., Sakurai Т., Nambu T. et al. Neurons containing orexin in the lateral hypothalamic area of the adult rat brain are activated by insulin-induced acute hypoglycemia//Neurosci Lett. 1999. Vol. 264. P. 101- 104.
  17. Perekrest S. V, Abramova Т. V, Novikova N. S. et al. Changes in immunoreactivity of orexin-A-positive neurons after an intravenous lipopolysaccharide injection // Med. Sci. Monitoring. 2008. Vol. 14. № 4.
  18. Peyron C, Tighe D. K., van den Pol A. N. et al. Neurons containing hypocretin (orexin) project to multiple neuronal systems // J. Neurosci. 1998. Vol. 18 (23). P. 9996-10015.
  19. Rybakina E. G., Shanin S. N, Kozinets I. A. et al. Cellular mechanisms of cold stress-related immunosupression and the action of interleukin 1 // Int. J. Tiss. Reac. 1997. XIX (3/4). P. 135-140.
  20. Sakamoto F, Yamada S., and Ueta Y. Centrally administered orexin-A activates corticotrophin-realising factor (CRF)-containing neurons in the hypothalamic paraventricular nucleus and central amygdaloid nucleus of rats // Regul. Pept. 2004. P. 118.
  21. Sakurai Т., Amemiya A., Ishii M. et al. Orexins and orexin receptors: a family of hypothalamic neuropeptides and G protein-coupled receptors that regulate feeding behavior // Cell. 1998. Vol. 92. P. 573-585.
  22. Shanin S. N. Natural killer cell cytotoxic activity and c-Fos protein synthesis in rat hypothalamic cells after painful electric stimulation of the hind limbs and EHF irradiation of the skin // Med. Sci. Monit. 2005. Vol. 11 (9).P.BR309-315.
  23. Sheridan J., Feng N, Bonneau R. et al. // J. Neuroimmunol. 1991. Vol. 31. № 2. P. 245-255.
  24. Swanson L. W. Organization of mammalian neuroendocrine systems // Handbook of Physiology. The Nervous System. Intrinsic Regulatory Systems of the Brain. Bethesda, MD: Am. Physiol. Soc. 1986. S. 1. Vol. IV. P. 17-363.
  25. Tamura R., Kondoh Т., Ono Т., Nishijo H, Tori K. Effects of repeated cold stress on activity of hypothalamic neurons in rats during performance of operant licking task //Am. Physiol. Society. 2000.
  26. Wenner M., Kawamura N., Ishikawa T. Reward linked to increased natural killer cell activity in rats // Neuroimmunol. Modulation. 2000. Vol. 7. P. 1-5.
  27. Wrona D., Trojniar W. Chronic electrical stimulation of the lateral hypothalamus in-creases natural Killer cell cytotoxicity in rats // J. Neurosci. 2000. Vol. 20 (17). P. 6578-6586.
  28. Yang Y. L. and Lu К. T. Effects of orexin-A on rat thermoregulation: the roles of prostaglandin E2 // Proceedings of the Australian Physiological and Pharmacological Society. 2008.



Abstract - 82


Article Metrics

Metrics Loading ...




  • There are currently no refbacks.

Copyright (c) 2009 Shainidze K.Z., Novikova N.S., Aleshina G.M., Darinsky J.A., Synchikova A.P., Korneva E.A.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies