Tissue matrices: New technologies to increase the diagnostic efficiency in oncogynecology

The method of tissue microarrays (TMA), invented by H. Battifora [1], was developing actively and modernized during last 20 years. Many aspects of this method have been studied: validation of the results of TMA technology to the results of the standard research methods, the expenses of TMA creation, variants of the estimation of the results of TMA research, tissue antigenicity preservation etc [4, 5,17]. Recently research of paraffin tissue banks in the aim of retrospective search for new tumor markers was considered as «a titanic work». Today TMA method makes this work a reality [12]. The authors of the present publication have used TMA method for studying of malignant gynecologic tumors in big cohorts of patients. High-throughput, estimated in practice, an opportunity of the fast standardized analyses of numerous patients for various biomarkers, economy and adequacy of TMA method allow to recommend this method for practical use. Application of TMA method in clinical histology expands the diagnostic opportunities for different kinds of pathology essentially and promotes the foundation of predictive medicine [14]. The speed with which new prognostic factors, defining the modality of therapy, are generated and transferred into clinical practice, demands the new methodological approaches enabling real-time or retrospective estimation of these clinically significant factors in numerous patients. Wide use ofTMA method will become an integrative part of work for scientific and routine clinical research in future.

tissue microarray, immunohistochemistry, histological technique, oncogynecology