ACCUMULATION OF POLYGLUTAMINE PROTEINS IN NEURONS OF THE HUMAN RED NUCLEUS

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Abstract


One of typical features of neurodegeneration is intranuclear accumulation of proteins containing polyglutamine sequence in their polypeptide chain. The aim of the present study was to identify such proteins, and to determine their distribution in the neurons of the red nucleus in humans not suffered from neurodegenerative diseases. Fragments of the human midbrain were used in the study (n=7). Polyglutamine sequence (poly-Q) in proteins was revealed using immunocytochemistry to monoclonal mouse antibody (clone 1C2). Analysis of the preparations revealed the presence of a weak diffuse reaction in the cell nucleus structures in most cells (both nerve and glial) of the red nucleus. In large neurons in two of the seven cases studied, the reaction of the intranuclear structures was diffuse, and in the other five cases, slightly contoured 1C2-immunopositive accumulations, always located outside of the nucleolus, were determined in the nucleus of large neurons. The obtained data suggest that the polyglutamine proteins typical for pathological structures can be accumulated in nucleus of nerve and glial cells of the red nucleus in humans without neurodegenerative pathology.

I P Grigorev

Institute of Experimental Medicine

M A Syrtsova

Institute of Experimental Medicine

E G Sukhorukova

Institute of Experimental Medicine

O V Kirik

Institute of Experimental Medicine

O S Alekseeva

Institute of Experimental Medicine

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Copyright (c) 2017 Grigorev I.P., Syrtsova M.A., Sukhorukova E.G., Kirik O.V., Alekseeva O.S.

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