THE ROLE OF CHLAMYDIA PNEUMONIAE AND INTERLEUKIN-8 IN DESTABILIZATION OF ATHEROSCLEROTIC PLAQUE IN HUMAN



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Abstract

Research objective: The aim of this work was a comparative study of the contents of the Chlamydia pneumoniae and IL-8 in unstable and stable atherosclerotic lesions in humans,and an assessment of their role in the destabilization of atherosclerotic plaques. Materials and methods: Investigated 38 aortic tissue samples received from 63±7 years men who died from coronary heart disease. Verification of unstable progressive atherosclerotic plaques was conducted using histological staining Oil Red O and hematoxylin Mayer. Chlamydia pneumoniae to identify modified histological method Romanowski-Giemsa. Determination of IL-8 was made highly sensitive two-stage biotin-streptavidin method. Analysis of the results was carried out using a light microscope Leica DM 2500 and computer program «Leica Application Suite Version 3.4.0». Results: In surface divisions of unstable atherosclerotic lesions of human arterial walls identified Chlamydia pneumoniae, expressed cellular products of IL-8 and mononuclear cells infiltrates consisting of lymphocytes and macrophages. Conclusion: The results can indicate a combined mutually enhances action Chlamydia pneumoniae and IL-8 in the formation of unstable atherosclerotic lesions.

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About the authors

P V Pigarevsky

Institute of Experimental Medicine

S V Maltseva

Institute of Experimental Medicine

V A Snegova

Institute of Experimental Medicine

N G Davydova

Institute of Experimental Medicine

O G Yakovleva

Institute of Experimental Medicine

R A Vorozhbit

Institute of Experimental Medicine

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Copyright (c) 2017 Pigarevsky P.V., Maltseva S.V., Snegova V.A., Davydova N.G., Yakovleva O.G., Vorozhbit R.A.

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