SEARCH OF THE NOVEL GENETIC DETERMINANTS IN STREPTOCOCCUS DYSGALACTIAE SUBSPECIES EQUISIMILIS

  • Authors: Dmitriev AV1,2, Kisely ov AM3, Kireeva AG1, Il’yasov Y.Y.1, Sergushichev AA4, Kazakov SV4, Kalinina OV3,5
  • Affiliations:
    1. Institute of Experimental Medicine
    2. St. Petersburg State Technological Institute (Technical University)
    3. Almazov North-West Federal Medical Research Centre
    4. ITMO University
    5. St. Petersburg Pasteur Institute
  • Issue: Vol 16, No 2 (2016)
  • Pages: 42-50
  • Section: Articles
  • URL: https://journals.eco-vector.com/MAJ/article/view/9495
  • DOI: https://doi.org/10.17816/MAJ16242-50
  • Cite item
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Abstract


Streptococcus dysgalactiae subsp. equisimilis (SDSE) previously considered to be a commensal bacterium, is currently recognized as a causative agent of about 60% of infections caused by groups C and G streptococci. Goal of the present study was to search the novel genetic determinants in the genome of four SDSE strains using next generation sequencing and bioinformatic analysis. As result, genome sequences of four SDSE strains were determined, and numerous DNA fragments previously uncharacterized for SDSE were revealed. Most of the fragments were presented by migrating genetic elements such as bacteriophages, transposons, plasmids, integrative conjugative elements etc. For the first time the resistance genes to antibacterial drugs (tetS, tetT - resistance to tetracycline, and lsaE, lnuB - to lincosamides) were determined in the studied SDSE strains. Given that horizontal gene transfer, in particular, virulence genes associated with migrating genetic elements, is a driving force of streptococcal evolution, an emergence of novel virulent SDSE clones is expected.

A V Dmitriev

Institute of Experimental Medicine; St. Petersburg State Technological Institute (Technical University)

Email: admitriev10@yandex.ru
St. Petersburg, Russia

A M Kisely ov

Almazov North-West Federal Medical Research Centre

Email: artem.kiselyov@gmail.com
St. Petersburg, Russia

A G Kireeva

Institute of Experimental Medicine

Email: nosik276@gmail.com
St. Petersburg, Russia

Yu Yu Il’yasov

Institute of Experimental Medicine

Email: kolpino@hotmail.com
St. Petersburg, Russia

A A Sergushichev

ITMO University

Email: alsergbox@gmail.com
St. Petersburg, Russia

S V Kazakov

ITMO University

Email: svkazakov@rain.ifmo.ru
St. Petersburg, Russia

O V Kalinina

Almazov North-West Federal Medical Research Centre; St. Petersburg Pasteur Institute

Email: olgakalinina@mail.ru
St. Petersburg, Russia

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Copyright (c) 2016 Dmitriev A.V., Kisely ov A.M., Kireeva A.G., Il’yasov Y.Y., Sergushichev A.A., Kazakov S.V., Kalinina O.V.

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