BACKGROUNDS The massive HIV-1 epidemic in Russia and the former USSR started in the mid-1990 years. In the early years of the epidemic when the leading risk factor was intravenous drug use, the population of HIV genetic variants was characterized by a high degree of homogeneity, with subtype was A1 being the most prevalent. In the late 90-es the recombinant form CRF03_AB was first registered in the Kaliningrad region; later on it was found as rare cases in all fUSSR countries, including Russia, and caused the outbreak in the city of Cherepovets in 2006. In addition, CRF02_AG recombinant, presumably originating from Cameroon, where this variant is quite widespread, was found in 2005 in Uzbekistan. The purpose of this study was to conduct an analysis of prevalence and nature of HIV-1 recombinant forms in Russia and fUSSR countries at the present time. METHODS From 2008 to 2015 years, 1347 sequences of HIV-1 pol gene from patients from Russia, Kazakhstan, Kyrgyzstan and Armenia were analyzed in the laboratory. The sequences were obtained using ViroSeq HIV-geno-typing system, as well as in house method. Genotyping and phylogenetic analysis were performed using the IDU-A CRFQ2AG Fig. l. The structure of URFs. 104 МЕДИЦИНСКИЙ АКАДЕМИЧЕСКИЙ ЖУРНАЛ, 2016 г., ТОМ 16, № 3 Fig. 2. The phylogenetic consensus tree of pol gene coding pol gene of HIV-1 from 1 to 99 aminoacids of protease and revertase from 1 to 232 aminoacids (according amino acid position relative to protein start in HXB2) performed by maximum-likelihood tree method (model GTR), using Mega 6.06 program. CRF02AG is picked by green color, CRF63 02A1 - by dark blue color, CRF03AB - by blue color, IDU-A variant - by red color and the unique recombinant forms by rhombuses. The references is designated its GenBank numbers. COMET HIV-1 / 2v.0.5, MEGA 6.06 and PhyML program. There were 141 recombinant forms found among the samples analyzed. In addition, 50 pol gene sequences of HIV-1 recombinants from GenBank (Russia, Ukraine, Belarus) were included into this study. RESULTS Three groups of circulating recombinant forms were found among the HIV-1 pol gene sequences analyzed - CRF02_AG (102/191, 53,4%), CRF03AB (37/191, 19,4%) and CRF63_02A1 (42/191, 22%) - the double recombinant generated by viruses belonging to subtype A1 and CRF02 AQ as well as 10 unique recombinant forms of the same origin. All CRF02 AG sequence from Russia and FSU without exception clustered with the variant of Uzbekistan. The frequency of recombinant forms of HIV-1 differed in different countries: we found 45.6% of them in Kyrgyzstan, 34.6% in Kazakhstan, 2.9% in Armenia and 4.5% in Russia. CONCLUSION Currently, the widespread of HIV-1 recombinant forms can be traced in all fUSSR countries and Russia, thereby increasing its diversity, with the appearance of unique recombinant forms. In general, it may be associated with the increased activity of migration, and with the active co-circulation of different HIV-1 genetic variants in the population.

V Laga

Ivanovsky Institute of Virology, Gamaleya research center of epidemiology and microbiology, Russian Ministry of health

E Kazennova

Ivanovsky Institute of Virology, Gamaleya research center of epidemiology and microbiology, Russian Ministry of health

M Bobkova

Ivanovsky Institute of Virology, Gamaleya research center of epidemiology and microbiology, Russian Ministry of health


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