EXAMINATION OF INFLAMMATORY MARKERS IN HIV-INFECTED PERSON WITH HEAVY ALCOHOL CONSUMPTION

  • Authors: Yaroslavtseva T1, Blokhina E1, Krupitsky EM1,2, Kaku S.3, Gnatienko N4, Palatkin V1, Toussova O1, Vetrova M1, Cheng DM3, Samet JH3,4, Matt F.5, Zvartau EE1, Lioznov DA1
  • Affiliations:
    1. First St. Petersburg Pavlov State Medical University
    2. St. Petersburg Bekhterev Research Psychoneurological Institute
    3. Boston University
    4. Boston Medical Center
    5. Vanderbilt University
  • Issue: Vol 16, No 3 (2016)
  • Pages: 118-119
  • Section: Articles
  • URL: https://journals.eco-vector.com/MAJ/article/view/9531
  • DOI: https://doi.org/10.17816/MAJ163118-119
  • Cite item

Abstract



Background - Inflammation (IL-6), altered coagulation (D-dimer) and monocyte activation (sCD14). • Increased in HIV. • Disrupted with heavy alcohol use. • Associated with mortality. - High density lipoproteins (HDL) have immuno-modulatory effects. - HIV decreases HDL cholesterol. • HDL cholesterol not fully restored with antiretroviral therapy. - Moderate alcohol intake increases HDL cholesterol. - HDL immuno-modulation. • Anti-inflammatory potential. - Anti-oxidative properties. - Reduce cytokines that drive immune cell adhesion. • Pro-inflammatory potential. Aims 1. To assess the combined effect of HIV infection and heavy alcohol use on HDL cholesterol levels: • Before antiretroviral therapy initiation; • After antiretroviral therapy initiation. 2. To assess whether HDL particle properties (cholesterol content, functionality) are associated with biomarkers of inflammation, altered coagulation, monocyte activation, cell adhesion among HIV infected recent drinkers. 3. To assess whether HDL cholesterol or HDL function mediates the association between heavy alcohol use and with biomarkers of inflammation, altered coagulation, monocyte activation, and cell adhesion among HIV+ recent drinkers. Significance This study will elucidate whether HDL dysfunction is an important driver of inflammation among HIV infected recent drinkers. Innovation Novel: • Measuring HDL functionality using a cell-free assay. • Simultaneous effect of HIV and alcohol on HDL functionality in HAART naïve people is under-studied. МЕДИЦИНСКИЙ АКАДЕМИЧЕСКИЙ ЖУРНАЛ, 2016 г., ТОМ 16, № 3 119 • The effect of HDL functionality on immuno-modulation in the context of HIV and alcohol use is under-studied. HDL function - Cell free assay. - Dihydrorhodamine 123 (DHR): • Fluorometric; • Measures oxidative potential of HDL. Russia ARCH (N=351) Inclusion criteria: • Age 18-70 years old; • HIV-infected; • Alcohol consumption; • Not on ART at the time of enrollment; • Provision of contact information for two contacts to assist with follow-up; • Possession of a home or mobile phone. Exclusion criteria: • Not fluent in Russian; • Cognitive impairment resulting in inability to provide informed consent. Alcohol Use • TLFB (30 days) • AUDIT (6 months) • Peth (Phosphatidylethanol) Study design Russia ARCH: Existing Data Study time point Completed n (%) Blood Specimen collection Dried Blood spot AUDIT TLFB Baseline 360 (100) / / / 12 months 240 (66.7) 24 105 / / / months (29.2) V V V Russia ARCH: New Data to be Measured Study time point Inflamm atory markers HDL Function PEth (alcohol bio marker) Baseline ✓ ✓ ✓ є months 12 ✓ ✓ ✓ months BIOMARKER FUNCTION IL-6 (lnterleukin-6) Systemic inflammation SCD14 (Soluble CD14) Monocyte activation D-dimer Altered coagulation MCP-1 (Monocyte chemoattractant protein-1) Regulates migration and infiltration of monocytes/macrophages sVCAM-1 (Soluble vascular cell adhesion molecule-1) Mediates leukocyte binding to endothelium

T Yaroslavtseva

First St. Petersburg Pavlov State Medical University

Russia

E Blokhina

First St. Petersburg Pavlov State Medical University

Russia

E M Krupitsky

First St. Petersburg Pavlov State Medical University; St. Petersburg Bekhterev Research Psychoneurological Institute

Russia

So-Armah Kaku

Boston University

USA

N Gnatienko

Boston Medical Center

USA

V Palatkin

First St. Petersburg Pavlov State Medical University

Russia

O Toussova

First St. Petersburg Pavlov State Medical University

Russia

M Vetrova

First St. Petersburg Pavlov State Medical University

Russia

D M Cheng

Boston University

USA

J H Samet

Boston University; Boston Medical Center

USA

Freiberg Matt

Vanderbilt University

Nashville, USA

E E Zvartau

First St. Petersburg Pavlov State Medical University

Russia

D A Lioznov

First St. Petersburg Pavlov State Medical University

Russia

Views

Abstract - 49

PDF (Russian) - 0

Cited-By


PlumX

Refbacks

  • There are currently no refbacks.

Copyright (c) 2016 Yaroslavtseva T., Blokhina E., Krupitsky E.M., Kaku S., Gnatienko N., Palatkin V., Toussova O., Vetrova M., Cheng D.M., Samet J.H., Matt F., Zvartau E.E., Lioznov D.A.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies