EXPERIMENTAL ACUTE STRESS AND MYOCARDIAL PROFIBROTIC PROCESS

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Abstract


This research investigates particularities of myocardial fibrogenesis following experimental adrenergic and cholinergic stress. The experiment used 2 series of Wistar rats (20 rats in a serie). 2 hours and 1 month after injection of medicament extracellular space density, collagen density; FGF-2, TGF-β1 cellular and endothelial expression was studied in right and left ventricle’s myocardium. Myocardial collagen density was significantly elevated both 2 hours and 1 month compared to intact animals following acute stress (both adrenergic and cholinergic variant). FGF-2 cellular expression was approximately the same 2 hours and 1 month following both stress variants. TGF-β1 cellular expression was significantly higher following cholinergic stress, than TGF-β1 expression following adrenergic stress (both 2 hours and 1 month after). Therefore, this findings may indicate that myocardial fibrogenesis following cholinergic stress proceeds more actively during first hours and persists 1 month after acute cholinergic stress (supported by prolonged TGF-β cellular expression). In our opinion, acute stress activates profibrotic process, wich, once initiated, could become self-supporting.

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About the authors

V R Veber

Yaroslav-the-Wise Novgorod State University

Email: viktor.veber@novsu.ru
Novgorod, Russia

M P Rubanova

Yaroslav-the-Wise Novgorod State University

Email: kafpdo@mail.ru
Novgorod, Russia

S V Zhmailova

Yaroslav-the-Wise Novgorod State University

Email: zhmailova.svetlana@yandex.ru
Novgorod, Russia

P M Gubskaya

Yaroslav-the-Wise Novgorod State University

Email: kafpdo@mail.ru
Novgorod, Russia

V E Karev

FSBEI SRI of Childhood infections of FMBA of Russia

Email: vadimkarev@yandex.ru
St.-Petersburg, Russia

Y Y Rumyantsev

Yaroslav-the-Wise Novgorod State University

Email: gogathejedi@gmail.com
Novgorod, Russia

N A Kulik

Yaroslav-the-Wise Novgorod State University

Email: nadinechist@mail.ru
Novgorod, Russia

D R Sulimanova

Yaroslav-the-Wise Novgorod State University

Email: kafpdo@mail.ru
Novgorod, Russia

References

  1. Rubanova M. P., Zhmaylova S. V., Antonov E. K. Change of extracellular spaces of myocardium change of myocardium in simulation of various options of acuta stress in the experiment // Vestnik of Novgorod State University.- 2011.- Vol. 62.- P. 83-86.
  2. Губская П. М., Вебер В. Р., Рубанова М. П. и др. Жидкостные пространства в миокарде крыс линии Вистар при моделировании хронического адренергического и холинергического стресса // Клиническая медицина. Межвузовский сборник стран СНГ.- Великий Новгород - Алматы, 2011.- Т. 19.- С. 92-97.
  3. Шабалин В. Н., Шатохина С. Н. Морфология биологических жидкостей человека.- М.: Хризостом, 2001.- 304 c.
  4. Shabalin V. N., Shatokhina S.N. Diagnostic markers in the structures of human biological liquids // Singapore med. J.- 2007.- Vol. 48.- P. 440-447.
  5. Автандилов Г.Г. Медицинская морфометрия. Руководство.- М.: Медицина, 1990.- С. 204-205.
  6. Целлариус Ю. Г., Семенова Л. А. Гистопатология очаговых метаболических повреждений миокарда.- Новосибирск: Наука, 1972.- C. 100-104.
  7. Koitabashi N. et. al. Pivotal role of cardiomyocyte TGF-β signaling in the murine pathological response to sustained pressure overload // J. Clin. Invest.- 2011.- Vol.121 (6).- P. 2301-2312.
  8. Huntgeburth M. et al. Transforming Growth Factor b1 Oppositely Regulates the Hypertrophic and Contractile Response to b-Adrenergic Stimulation in the Heart // PLoS ONE 6(2011): e26628. doi: 10.1371/journal.pone.0026628. http://www.plosone.org/article/info:doi/10.1371/journal.pone.0026628
  9. Yoshimatsu Y., Watabe T. Roles of TGF-β Signals in Endothelial-Mesenchymal Transition during Cardiac Fibrosis // International Journal of Inflammation.- 2011.- Vol. 10.- P. 1-8.
  10. Piera-Velazquez S., Li Z., Jimenez S. A. Role of endothelial-mesenchymal transition (EndoMT) in the pathogenesis of fibrotic disorders // Am. J. Pathol.- 2011.- Vol. 179 (3).- P. 1074-1080.
  11. Лобзин Ю. В., Вебер В. Р., Карев В. Е. и др. Экспрессия коллагена I типа эндотелиоцитами сосудов миокарда при адренергическом и холинергическом вариантах острого стресса в эксперименте // Мат-лы VII Национального конгресса терапевтов.- М., 2012.- C. 119-120.
  12. Румянцев Е. Е., Вебер В. Р., Рубанова М. П. и др. Активность эндотелиоцитов сосудов миокарда в фибропластическом процессе при остром стрессе в эксперименте // Мат-лы VII Национального конгресса терапевтов.- М., 2012.- C. 177.

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Copyright (c) 2014 Veber V.R., Rubanova M.P., Zhmailova S.V., Gubskaya P.M., Karev V.E., Rumyantsev Y.Y., Kulik N.A., Sulimanova D.R.

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