THYMIC MAST CELLS AS PARTICIPANTS IN NEURO-IMMUNE INTERACTIONS



Cite item

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription or Fee Access

Abstract

Studying of thymic mast cells population in normal state and after stress-induced atrophy was a background of this work. Work is performed on 60 thymus of white outbred mice with using of histochemistry and immunohistochemistry methods. Adult mice were given a single injection of 2,5 mg of hydrocortisone for induction of thymic accidental transformation; sections were stained with toluidine blue and alcian blue-safranin. Immunohistochemical reactions for the synaptophysin or tyrosine hydroxylase with alcian blue stain were used for identification of nerve terminals in adult thymus. In adult animals MCs were observed only in the connective tissue of the capsule, interlobular septa, subcortex and perivascular space. Mast cells mature in thymus after accidental transformation. The localization of developing mast cells was changing from medullar and cortical to capsular. A morphological proximity between nerve terminals and mast cells have been observed in normal adult thymus. Some of these nerves are catecholaminergic. Possible important role of thymic mast cells and mast cells-nerves interaction in normal state and after accidental transformation is discussed.

Full Text

Restricted Access

About the authors

V V Guselnicova

St.-Petersburg State University

St.-Petersburg, Russia

E G Sukhorukova

Institute of Experimental Medicine of the North-West branch of the Russian Academy of Medical Sciences

St.-Petersburg, Russia

D E Korzhevsky

Institute of Experimental Medicine of the North-West branch of the Russian Academy of Medical Sciences

St.-Petersburg, Russia

A V Polevschikov

Institute of Experimental Medicine of the North-West branch of the Russian Academy of Medical Sciences; St.-Petersburg State University; Far Eastern Federal University

St.-Petersburg, Russia; Vladivostok, Russia

References

  1. Корнева Е. А., Хай Л. М. Влияние разрушения участков гипоталамической области на процесс иммуногенеза // Физиол. журнал СССР.- 1963.- Т. 49, № 1.- С. 42-48.
  2. Пуговкин А. П. Морфофункциональные основы обеспечения нейрогуморальной регуляции функций иммунной системы // Иммунофизиология / под ред. Е. А. Корневой.- СПб.: Наука, 1993.- С. 37-66.
  3. Bulloch K. Neuroanatomy of lymphoid tissue: a review // Neural modulation of immunity / Proc. Int. Symp. / Eds. R. Guillemin, M. Cohn.-N.Y., 1985.- P. 111-141.
  4. Felten D. L., Felten S. Y., Carlson S. L. et al. Noradrenergic and peptidergic innervation of lymphoid tissue // J. Immunol.- 1985.- Vol. 135, № 2.- P. 755S-765S.
  5. Livnat S., Felten S. Y., Carlson S. L. et al. Involvement of peripheral and central catecholamine systems in neural-immune interactions // J. Neuroimmunol.- 1985.- Vol. 10, № 1.- P. 5-30.
  6. Williams J. M., Peterson R. G., Shea P. A. et al. Sympathetic innervation of murine thymus and spleen: evidence for a functional link between the nervous and immune systems // Brain Res. Bull.- 1981.- Vol. 6, № 1.- P. 83-94.
  7. Юшков Б. Г., Черешнев В. А., Климин В. Г., Арташян О. С. Тучные клетки: физиология и патофизиология.- М.: Медицина, 2011.-237 с.
  8. Гусельникова В. В., Синицина В. Ф., Королькова Е. Д. и др. Локализация тучных клеток в тимусе мыши на разных этапах онтогенеза // Морфология.- 2012.- Т. 141, № 2.- С. 40-45.
  9. Befus A. D., Johnston N., Nielsen L. et al. Thymic mast cell deficiency in avian muscular dystrophy // Thymus.- 1981.- Vol. 3, № 6.-P. 369-376.
  10. Bigaj J., Urbanska-Stopa M., Plyrycz B. Argentaffin mast cell in the thymus of the frog // Folia Histochem. Cytobiol.- 1999.- Vol. 29, №1.- P. 45-47.
  11. Bodey B., Calvo W., Prummer O. et al. Development and histogenesis of the thymus in dog: a light and electron microscopical study // Dev. Com. Immunol.- 1987.- Vol. 11, № 1.- P. 227-238.
  12. Lorton D., Bellinger D. L., Felten S. Y., Felten D. L. Substance P innervation of the rat thymus // Peptides.- 1990.- Vbl. 11.- P. 1269-1275.
  13. Kendall M. D. Hemopoiesis in the thymus // Dev. Immunol.- 1995.- Vol. 4, № 1.- P. 157-168.
  14. Арташян О. С., Юшков Б. Г., Храмцова Ю. С. Морфологические аспекты участия тучных клеток в формировании общего адаптационного синдрома // Таврический медико-биологический вестник.- 2012.- Т. 15, № 3.- С. 22-25.
  15. Кветной И. М., Ярилин А. А., Полякова В. О., Князькин И. В. Нейроиммуноэндокринология тимуса.- СПб.: ДЕАН, 2005.- 160 с.
  16. Rychter J. W., Nassauw L. van, Timmermans J. P. et al. CGRP1 receptor activation induces piecemeal release of protease-1 from mouse bone marrow-derived mucosal mast cells // Neurogastroenterol. Motil.- 2011.- Vol. 23, № 2.- P. 57-68.
  17. Gottwald P., Hewlett B. R., Lhotak S., Stead R. H. Electrical stimulation of the vagus nerve modulates the histamine content of mast cells in the rat jejunal mucosa // Neuroreport.- 1995.- Vol. 7.- P. 313- 317.
  18. Vliagoftis H., Dimitriadou V., Theoharides T. C. Progesterone triggers selective mast cell secretion of 5-hydroxytryptamine // Int. Arch. Allergy Appl. Immunol.- 1990.- Vol. 93.- P. 113-119.
  19. Старская И. С., Кудрявцев И. В., Полевщиков А. В. Морфологический анализ и динамика гидрокортизон-индуцированной атрофии тимуса // Медицинский академический журнал.- 2012.- Т. 12, № 3.- С. 94-96.
  20. Marinova T., Philipov S., Aloe L. Nerve Growth Factor immunoreactivity of mast cells in acute involuted human thymus // Inflammation.- 2007.- Vol. 30, № 1-2.- P. 38-43.
  21. Aloe L., DeSimone R. NGF primed spleen cells injected in brain of developing rats differentiate into mast cells // Int. J. Dev. Neuroscience.- 1989.- Vol. 7.- P. 565-573.
  22. Desaga J. F., Parwaresch M. R., Muller-Hermelink H. K. Die Zytochemische Identifikation der Mastzellvorstuffen bei der Ratte // Z. Zellforsch.- 1971.- Vol. 121.- P. 292-300.
  23. Spicer S. S. A correlative study of the histochemistry properties of rodent acid mucopolysaccharides // J. Histochem. Cytochem.- 1960.-Vol. 8.- P. 18-35.
  24. Chen Z., Wang G., Luo H. et al. Chronic stress induced proliferation and activation of mast cells of gastric antrum in rats // Wuhan University Journal of Natural Sciences.- 2008.- Vol. 13, № 2.- P. 247-251.
  25. Гусельникова В. В., Пронина А. П., Назаров П. Г., Полевщиков А. В. Происхождение тучных клеток: современное состояние проблемы // Вопросы морфологии XXI века.- 2010.- Вып. 2.- C. 108-115.
  26. Ginsburg H. The in vitro differentiation and culture of normal mast cells from the mouse thymus // Ann. N. Y. Acad. Sci.- 1963.- Vol. 103, № 1.- P. 20-39.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2013 Guselnicova V.V., Sukhorukova E.G., Korzhevsky D.E., Polevschikov A.V.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: серия ПИ № ФС 77 - 74760 от 29.12.2018 г.


This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies