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Streptococcus pyogenes is well-known human pathogen. The transcriptional regulatory protein Rgg controls the variety of S. pyogenes phenotypes, in particular, virulent phenotype. In this study the rgg gene was restored in SF370 rgg- и NZ131 rgg- mutant strains under native and plasmid heterologous promoters. Transcriptional level of rgg gene under the control of heterologous promoter was 2-fold higher than that under the control of native promoter. Restoration of rgg in the chromosome under the control of the native promoter restored wild-type phenotypes. In contrast, episomal complementation of rgg under plasmid heterologous promoter did not restore any of phenotypic properties. Together, the results indicate that the mechanism of Rgg-mediated regulation is complex. Given the importance of Rgg in controlling virulence properties in S. pyogenes, further characterization of the mechanism of Rgg-mediated gene transcription is necessary.

A V Dmitriev

Institute of Experimental Medicine of the North-West Branch of the Russian Academy of Medical Sciences; St.-Petersburg State University

St.-Petersburg, Russia

M S Chaussee

University of South Dakota

Vermillion, USA

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