DEPENDENCE OF STREPTOCOCCUS PYOGENES PROPERTIES FROM THE RGG GENE TRANSCRIPTIONAL LEVEL
- Authors: Dmitriev AV1,2, Chaussee MS3
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Affiliations:
- Institute of Experimental Medicine of the North-West Branch of the Russian Academy of Medical Sciences
- St.-Petersburg State University
- University of South Dakota
- Issue: Vol 13, No 3 (2013)
- Pages: 114-119
- Section: Articles
- Published: 15.09.2013
- URL: https://journals.eco-vector.com/MAJ/article/view/9854
- DOI: https://doi.org/10.17816/MAJ133114-119
- ID: 9854
Cite item
Abstract
Streptococcus pyogenes is well-known human pathogen. The transcriptional regulatory protein Rgg controls the variety of S. pyogenes phenotypes, in particular, virulent phenotype. In this study the rgg gene was restored in SF370 rgg- и NZ131 rgg- mutant strains under native and plasmid heterologous promoters. Transcriptional level of rgg gene under the control of heterologous promoter was 2-fold higher than that under the control of native promoter. Restoration of rgg in the chromosome under the control of the native promoter restored wild-type phenotypes. In contrast, episomal complementation of rgg under plasmid heterologous promoter did not restore any of phenotypic properties. Together, the results indicate that the mechanism of Rgg-mediated regulation is complex. Given the importance of Rgg in controlling virulence properties in S. pyogenes, further characterization of the mechanism of Rgg-mediated gene transcription is necessary.
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About the authors
A V Dmitriev
Institute of Experimental Medicine of the North-West Branch of the Russian Academy of Medical Sciences; St.-Petersburg State University
Email: admitriev10@yandex.ru
St.-Petersburg, Russia
M S Chaussee
University of South DakotaVermillion, USA
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