Effect of intratumoral implantation of barium hexaferrite, magnetite, hematite, aluminium oxide and silica on the dynamics of Erlich tumor growth and survival value of tumor-carrying mice

Cover Page


Cite item

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription or Fee Access

Abstract

Introduction. We have earlier shown antitumoral effect of barium hexaferrite implant BHF for models of two intratumoral transplantable solid tumor of mice — LIO-1 and Erlich (2 sm3 tumors). In the mentioned work on the model of a rather big sized solid Erlich tumor (3 sm3) we have studied a comparative antitumoral effect after the implantation of BHF particles with a residual magnetic field of 150 mGsm referring to particles of iron oxides with residual magnetic field that was nearing to the zerohematite (Fe2O3) and magne — tite (Fe3O4), and as well as for the control — aluminium oxide (Al2O3) and silica (nSiO2 ∙ mH2O).

Material and methods. Trials have been conducted with 70 white mice — female mice with a mass of 23–24 g. Malignant epithelial tumor of Erlich was intramuscularly transplanted into the right hind extremity. When tumors reached a size of 3 sm3, mice were divided into 6 groups whom BHF, magnetite, hematite, aluminium oxide, silica or physiologic saline were implanted, correspondingly.

Results. As a result of the conducted studies it was found that BHF being implanted into a big-sized tumor (3 sm3) has a mild antitumoral effect for all the studies periods. Particles of iron oxides — magnetite and hematite — with a magnetic field nearing the zero did not possess the antitumor effect. By the end of experiment (29 days) in all groups including a control group, death of mice at 20 to 50 percent have been seen, and tumors were significantly necrotized, whereas in a group with implantation of silica into the tumor 100 percent of mice have survived and tumors itself did not have necrosis and had even pink colouring.

Full Text

Restricted Access

About the authors

Sofia F. Vershinina

Federal State Budget Institution Acad. A.M. Granov Russian Research Centre оf Radiology and Surgical Technologies Ministry of Healthcare of Russia

Author for correspondence.
Email: sofia.vershinina2010@mail.ru
ORCID iD: 0000-0002-8751-9168
SPIN-code: 5864-5627

leading researcher

Russian Federation, Saint Petersburg

Vladimir I. Evtushenko

Federal State Budget Institution Acad. A.M. Granov Russian Research Centre оf Radiology and Surgical Technologies Ministry of Healthcare of Russia

Email: sofia.vershinina2010@mail.ru
ORCID iD: 0000-0002-1577-4178
SPIN-code: 6167-0141

head researcher

Russian Federation, Saint Petersburg

References

  1. Заридзе Д.Г., Каприн А.Д., Стилиди И.С. Динамика заболеваемости злокачественными новообразованиями и смертности от них в России // Вопросы онкологии. – 2018. – Т. 64. – № 5. – С. 578–591. [Zaridze DG, Kaprin AD, Stilidi IS. Dynamics of morbidity and mortality from malignant tumors in Russia. Vopr Onkol. 2018;64(5):578-591. (In Russ.)]
  2. Каприн А.Д., Старинский В.В., Петрова Г.В. Злокачественные новообразования в России в 2016 году (заболеваемость и смертность). – М.: МНИОИ им. П.А. Герцена, 2018. – 250 с. [Kaprin AD, Starinskiy VV, Petrova GV. Zlokachestvennye novoobrazovaniya v Rossii v 2016 godu (zabolevaemost’ i smertnost’). Moscow: MNIOI im. P.A. Gertsena; 2018. 250 p. (In Russ.)]
  3. Гершанович М.Л., Филов В.А., Акимов М.А., Акимов А.А. Введение в фармакотерапию злокачественных опухолей. – СПб.: СОТИС, 1999. – 152 с. [Gershanovich ML, Filov VA, Akimov MA, Akimov AA. Vvedenie v farmakoterapiyu zlokachestvennykh opukholey. Saint Petersburg: SOTIS; 1999. 152 p. (In Russ.)]
  4. Евтушенко В.И., Вершинина С.Ф., Гранов А.М. Подавление роста и метастазирования злокачественных новообразований с помощью внутриопухолевой имплантации гексаферрита бария // Сибирский онкологический журнал. – 2009. – № S2. – С. 68. [Evtushenko VI, Vershinina SF, Granov AM. Podavlenie rosta i metastazirovaniya zlokachestvennykh novoobrazovaniy s pomoshch’yu vnutriopukholevoy implantatsii geksaferrita bariya. Sibirskii onkologicheskii zhurnal. 2009;(S2):68. (In Russ.)]
  5. Гранов А.М., Вершинина С.Ф., Маркочев А.Б., и др. Оценка противоопухолевого эффекта ферромагнитного имплантата в эксперименте // Медицинский академический журнал. – 2011. – Т. 11. – № 4. – С. 51–57. [Granov AM, Vershinina SF, Markochev AB, et al. Evaluation of antitumoral effect of ferromagnetic implant in the experiment. Med Akad Z. 2011;11(4):51-57. (In Russ.)]
  6. Гранов А.М., Карелин М.И., Гранов Д.А., и др. Магнитожесткий ферромагнетик в экспериментальной онкологии и клинике / под ред. А.М. Гранова. – СПб.: Фолиант, 2015. – 120 с. [Granov AM, Karelin MI, Granov DA, et al. Magnitozhestkiy ferromagnetik v eksperimental‘noy onkologii i klinike. Ed. by A.M. Granov. Saint Petersburg: Foliant; 2015. 120 p. (In Russ.)]
  7. Гранов А.М., Вершинина С.Ф., Самсонов Р.Б., и др. Противоопухолевый эффект имплантирования гексаферрита бария мышам SHR с односторонней и билатеральной лимфосаркомой ЛИО-1 // Медицинский академический журнал. – 2017. – Т. 17. – № 1. – С. 82–87. [Granov AM, Vershinina SF, Samsonov RB, et al. Antineoplastic effect of implantation of barium hexaferrite to shr mice suffering with unilateral and bilateral lymphosarcoma LIO-1. Med Akad Z. 2017;17(1):82-87. (In Russ.)]
  8. Kim JS, Yoon TJ, Yu KN, et al. Toxicity and tissue distribution of magnetic nanoparticles in mice. Toxicol Sci. 2006;89(1):338-347. https://doi.org/10.1093/toxsci/kfj027.
  9. Вершинина С.Ф., Стуков А.Н. Экспериментальные опухоли: практическое руководство. – СПб.: Галаника, 2018. – 68 с. [Vershinina SF, Stukov AN. Eksperimental’nye opukholi. Prakticheskoe rukovodstvo. Saint Petersburg: Galanika; 2018. 68 p. (In Russ.)]
  10. Стуков А.Н., Иванова М.А., Никитин А.К., и др. Индекс роста опухоли как интегральный критерий эффективности противоопухолевой терапии в эксперименте // Вопросы онкологии. – 2001. – Т. 47. – № 5. – С. 616–618. [Stukov AN, Ivanova MA, Nikitin AK, et al. Indeks rosta opukholi kak integral’nyy kriteriy effektivnosti protivoopukholevoy terapii v eksperimente. Vopr Onkol. 2001;47(5):616-618. (In Russ.)]

Supplementary files

Supplementary Files
Action
1. JATS XML
Download
2. Fig. 1. Microphotoes of the water suspension of particles being used as transplant: a — barium hexaferrite; b — magnetite; c — hematite; d — aluminium oxide; e — silica. Enlargement of ×40, scale — 20 micrometers

Download (956KB)
Indexing metadata
3. Fig. 2. Influence of BHF and silica on the dynamics of the growth (volume) of solid Erlich tumor in white female mice at implanting in the centre of the tumor node. 0 — a day of the transplantation of Erlich tumor. On the axis abscissa — days after transplantation of the tumor. The arrow shows the day of implanting BHF or silica in the tumor centre. I — control mice (injection of isotonic sodium chloride solution); II — BHF; III — silica

Download (93KB)
Indexing metadata
4. Fig. 3. Influence of implants of magnetite, hematite and aluminium oxide on the dynamics of the growth of Erlich solid tumor in white female mice. 0 — initial point of the trial — transplantation of Erlich tumor. On the axis abscissa — days after transplantation of the tumor. Vertical arrow — a day of implanting magnetite, hematite or aluminium oxide in the centre of tumor. I — control mice (isotonic sodium chloride solution); II — magnetite; III — hematite; IV — aluminium oxide. The difference between control and experienced groups for all the duration of the study is statistically unreliable

Download (88KB)
Indexing metadata
5. Fig. 4. Tumor of Erlich. 29th days after transplantatio tumoris. On the left are tumor cages. On the right are tumor nekroz. Colouring by hematoxylin and eosin, ×50

Download (93KB)
Indexing metadata

Copyright (c) 2020 Vershinina S.F., Evtushenko V.I.

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: серия ПИ № ФС 77 - 74760 от 29.12.2018 г.