Vol 20, No 1 (2020)

The aim of the study is the modern scientific literature estimation in the field of the investigation of neurodegenerative and metabolic disorders mediated by the trace amines and their receptors. The analysis of modern ideas about the “feedback” of neurodegenerative and metabolic diseases in which the trace amines and their receptors are involved was carried out. The important role of trace amines and their receptors in the regulation of the dopamine system, in connection with metabolic and neurodegenerative diseases, including Parkinson’s disease, ADHD, schizophrenia, obesity, metabolic syndrome and other pathological conditions, has been shown. Trace amines and their receptors have a direct effect on dopamine systems, being regulators of various metabolic and neurodegenerative processes, participating in energy metabolism, neurogenesis, and other vital processes.

Incidence of Parkinson disease progressively grows with increasing age and percentage of elderly people in the global population. Clear understanding of the causes of dopaminergic neurons’ death in Substantia nigra and Parkinson disease pathogenesis are currently absent, not speaking of an efficient therapy. However, an early diagnosis of dopaminergic neurons’ degeneration and prescription of dopamine replacement therapy significantly slow down the rate of symptoms’ progression. An increased concentration of iron in Substantia nigra of Parkinson disease patients has been shown in several studies. In this review we summarized the data concerning a potential significance of lactoferrin, the iron-binding protein of exocrine secretions and neutrophils, for early diagnosis and treatment of Parkinson disease. Salivary and lacrimal lactoferrin levels in Parkinson disease patients were higher than those observed in the control group. Plasma levels of lactoferrin inversely correlated with Parkinson disease severity even after treatment with Levodopa, a dopamine agonist, and with monoaminooxidase inhibitors. Lactoferrin levels in cerebrospinal fluid of Parkinson disease patients negatively correlated with the tumor necrosis factor-alpha concentration. Lactoferrin treatment of rodents with several experimental models of Parkinson disease (induced by rotenone, MPTP) protected neurons and mitigated the symptoms of neurodegeneration. Some contradictions about the positive effects of lactoferrin as a remedy in Parkinson disease animal models and possible participation of lactoferrin in accumulation of iron in neurons are discussed.

Objective: to develop algorithm of correlation analysis of prospective non-randomized clinical trial AMIRI–CABG (ClinicalTrials.gov Identifier: NCT03050489) data using SAS Enterprise Guide 6.1.

Materials and methods. Data collection was performed according prospective non-randomized clinical trial AMIRI–CABG (ClinicalTrials.gov Identifier: NCT03050489) in Pavlov First Saint Petersburg State Medical University, Saint Petersburg, Russia between 2016–2019 years with 336 patients. There is database with clinical, laboratory and instrumental data. Correlation analysis was performed with SAS Enterprise Guide 6.1.

Results. There was developed algorithm of correlation analysis data of prospective non-randomized clinical trial AMIRI–CABG (ClinicalTrials.gov Identifier: NCT03050489). This algorithm could be useful for physicians and researchers for data analysis.

Conclusion. Presented algorithm of correlation analysis could make easier and improve efficient data analysis with SAS Enterprise Guide 6.1.

The aim of the article — to study the effect of insulin on apolipoprotein A-I gene expression level in human macrophages and to reveal the main signal cascades which take part in the insulin-mediated regulation of apolipoprotein A-I gene.

Materials and methods. The experiments were carried out on the macrophages differentiated from acute monocytic leukemia cell line THP-1 and on the macrophages differentiated from the monocytes isolated from peripheral human blood. The analysis of apoA-I gene expression was performed by RealTime RT-PCR (on the mRNA level) and by flow cytofluorometry. To study the signalling cascades which take part in the insulin-mediated regulation of apoA-I gene the inhibitory analysis was used.

Results. Insulin induces the human apoA-I gene transcription in macrophages, but decreases the level of the ApoA-I protein which binds to outer cytoplasmic membrane of macrophages. The insulin-mediated transcription of apoA-I gene depends on PI3K-AKT signal cascade and transcription factors NF-κB and LXRs.

Conclusions. Taking into account our previous data it is plausible to conclude that the elevation of ApoA-I mRNA in human macrophages after insulin treatment leads to an increase of the amplitude of macrophages anti-inflammatory response, which consists in a sharp rise in the level of surface ApoA-I in macrophages under the some proinflammatory stimuli (TNFα, LPS).

The aim of the study was to compare the ability of various media for cryopreservation of sperm to ensure their viability after thawing and to assess the possibility of using Narcissus pseudonarcissus lectin to determine the viability of native and cryopreserved human sperm by flow cytometry.

Materials and methods. Used ejaculate 54 men aged 26 to 47 years, undergoing treatment for infertility. The control was a native ejaculate, which was also used for the in vitro fertilization procedure. Four parallel samples were frozen using various commercial media. After storage and thawing, spermatozoa viability was assessed by flow cytometry using three dyes and Narcissus pseudonarcissus lectin.

Results. All assays showed that cryopreservation led to a twofold decrease of sperm viability, dye to the changes in the composition and properties of cell membrane, decrease in mitochondrial membrane potential, as well as the damages of acrosomal complex and nucleus. The lowest decrease in sperm viability was shown for Quinn’s advantage sperm freezing medium for cryopreservation.

Conclusion. Flow cytometry makes it possible to evaluate with high efficiency sperm viability as the part of in vitro fertilization. The results of viability assessment using daffodil lectin make the prediction of in vitro fertilization outcome more accurate.

In recent years, the problem of vascular cognitive impairment is becoming increasingly important due to the increasing prevalence of cerebrovascular disease. The aim of this study was to determine the relationship between 25(OH)D, highly sensitive CRP, interleukin-1β, interleukin-6 and cognitive function in patients with cerebrovascular disease and to assess their impact on quality of life.

Materials and methods. 58 patients with cerebrovascular disease aged 31–81 years were examined. The comparison group consisted of 24 patients, comparable in age and sex, without the studied pathology. In the study, patients were determined by the level of 25(OH)D, interleukin-1β, interleukin-6, highly sensitive CRP in peripheral blood. All patients underwent neuropsychological, psychometric examination and study of their quality of life.

Results. In the study, the level of 25(OH)D in patients with cerebrovascular disease was lower compared to the group without this pathology. Concentrations of interleukin-1β, interleukin-6, and highly sensitive CRP in patients in the main group were statistically significantly higher than in those in the comparison group. Higher concentrations of highly sensitive CRP, interleukin-1β, and interleukin-6 were found in patients with low vitamin D availability. The study obtained direct correlations between the level of 25(OH)D in peripheral blood and the results of neuropsychological testing (MMSE, MoCA, FAB, Schulte tables (performance), the “Drawing hours” test) and the inverse with the degree of workability (Schulte tables). An inverse correlation of average strength was observed between the results of psychometric testing and the level of 25(OH)D in peripheral blood. The study confirmed the relationship between the level of 25(OH)D in peripheral blood and quality of life indicators, in the form of a direct correlation.

Conclusion. The study found that the higher the concentration of 25 (OH)D and the lower the level of interleukin-1β, interleukin-6, and highly sensitive CRP in the peripheral blood, the less likely it is to develop cognitive and psychoemotional disorders. The study confirmed the relationship between the level of 25(OH)D and quality of life indicators.