Polymorphism Glu298Asp of endothelial nitric oxide synthase gene and arterial hypertension

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Abstract

The aim of the study was the analysis of the influence of polymorphism Glu298Asp of endothelial nitric oxide synthase gene in patients with arterial hypertension (AH). The study included 98 AH patients at stage // and //I (male, Russian nationals, average age 53,2). The following analyses were performed: clinical analysis of the blood, general analysis of the urine, lipid spectrum, plasma electrolytes, creatinine, glucose, electrocardiography, echocardioscopy, examination of the eye vessels, ultrasound examination of the carotid arteries, microalbuminuria. Other risk factors were taken into consideration (hereditary predisposition, smoking, obesity). The polymorphism Glu298Asp of endothelial nitric oxide synthase gene was detected with two methods: polymerase chain reaction and restrictase reaction. The control group for Glu298Asp polymorphism detection included 102 healthy Russian donors aged 18 to 50. Statistical significance of the differences was probed with the ч2 criterion. The genotypes ratios in AH patients were as follows: GG 54%, GA 36%, AA 10%, in the control group: GG 53%, GA 36%, AA 11%. The polymorphism Glu298Asp of endothelial nitric oxide synthase gene was independent of such risk factors as hereditary predisposition to AH, dyslipidemia, obesity, diabetes mellitus. Among the target organ pathology in AH, retinal angiopathy, carotid arteries atherosclerosis, hypertrophy of the left ventricle and microalbuminuria were found to be independent of the polymorphism Glu298Asp of endothelial nitric oxide synthase gene. The Glu298Asp polymorphism did not affect the incidence of associated clinical complications, such as obliterating atherosclerosis of the lower extremity vessels, ischemic heart disease, and acute insufficiency of cerebral circulation, whereas chronic heart failure had a significantly lower incidence in the group of AA homozygotes as compared to patients with GA and GG genotypes.

About the authors

T. Yu. Kuznetsova

Karelian scientific medical center NWB RAMS

Author for correspondence.
Email: medaj@eco-vector.com
Russian Federation, Petrozavodsk

I. P. Dudanov

Karelian scientific medical center NWB RAMS

Email: medaj@eco-vector.com

член-корреспондент РАМН

Russian Federation, Petrozavodsk

D. V. Gavrilov

Karelian scientific medical center NWB RAMS

Email: medaj@eco-vector.com
Russian Federation, Petrozavodsk

A. V. Balatski

Lomonosov Moscow State University

Email: medaj@eco-vector.com

Faculty of fundamental medicine

Russian Federation, Moscow

P. I. Makarevich

Lomonosov Moscow State University

Email: medaj@eco-vector.com

Faculty of fundamental medicine

Russian Federation, Moscow

E. V. Parfjonova

Lomonosov Moscow State University

Email: medaj@eco-vector.com

Faculty of fundamental medicine

Russian Federation, Moscow

L. M. Samokhodskaja

Lomonosov Moscow State University

Email: medaj@eco-vector.com

Faculty of fundamental medicine

Russian Federation, Moscow

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Copyright (c) 2007 Kuznetsova Т. Yu., Dudanov I. Р., Gavrilov D. V., Balatski A. V., Makarevich P. I, Parfjonova E. V., Samokhodskaja L. M.


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