ACCUMULATION OF POLYGLUTAMINE PROTEINS IN NEURONS OF THE HUMAN RED NUCLEUS
- Authors: Grigorev IP1, Syrtsova MA1, Sukhorukova EG1, Kirik OV1, Alekseeva OS1
-
Affiliations:
- Institute of Experimental Medicine
- Issue: Vol 17, No 2 (2017)
- Pages: 76-79
- Section: Articles
- Published: 15.06.2017
- URL: https://journals.eco-vector.com/MAJ/article/view/9432
- DOI: https://doi.org/10.17816/MAJ17276-79
- ID: 9432
Cite item
Abstract
One of typical features of neurodegeneration is intranuclear accumulation of proteins containing polyglutamine sequence in their polypeptide chain. The aim of the present study was to identify such proteins, and to determine their distribution in the neurons of the red nucleus in humans not suffered from neurodegenerative diseases. Fragments of the human midbrain were used in the study (n=7). Polyglutamine sequence (poly-Q) in proteins was revealed using immunocytochemistry to monoclonal mouse antibody (clone 1C2). Analysis of the preparations revealed the presence of a weak diffuse reaction in the cell nucleus structures in most cells (both nerve and glial) of the red nucleus. In large neurons in two of the seven cases studied, the reaction of the intranuclear structures was diffuse, and in the other five cases, slightly contoured 1C2-immunopositive accumulations, always located outside of the nucleolus, were determined in the nucleus of large neurons. The obtained data suggest that the polyglutamine proteins typical for pathological structures can be accumulated in nucleus of nerve and glial cells of the red nucleus in humans without neurodegenerative pathology.
Full Text
![Restricted Access](https://journals.eco-vector.com/lib/pkp/templates/images/icons/text_lock.png)
About the authors
I P Grigorev
Institute of Experimental Medicine
M A Syrtsova
Institute of Experimental Medicine
E G Sukhorukova
Institute of Experimental Medicine
O V Kirik
Institute of Experimental Medicine
O S Alekseeva
Institute of Experimental Medicine
References
- Habas C., Guillevin R., Abanou A. In vivo structural and functional imaging of the human rubral and inferior olivary nuclei: a mini-review // Cerebellum. 2010. Vol. 9, No 2. P. 167-173.
- Dwork A.J., Schon E.A., Herbert. J. Nonidentical distribution of transferrin and ferric iron in human brain // Neurosci. 1988. Vol. 27, No 1. P. 333-345.
- Uchihara T., Fujigasaki H., Koyano S. Yagishita S., Iwabuchi K. Non-expanded polyglutamine proteins in intranuclear inclusions of hereditary ataxias-triple-labeling immunofluorescence study // Acta Neuropathol. 2001. Vol. 102, No 2. P. 149-152.
- Blum E.S., Schwendeman A.R., Shaham S. PolyQ disease: misfiring of a developmental cell death program? // Trends Cell Biol. 2013. Vol. 23. P. 168-174.
- Григорьев И.П., Коржевский Д.Э. Тельца Маринеско - внутриядерные включения дофаминергических нейронов // Мед. акад. журн. 2015. Т. 15, № 2. С. 28-34 [Grigorev I.P., Korzhevskii D.E. Marinesco bodies: intranuclear inclusion-bodies of dopaminergic neurons, Med. Acad. Journal. 2015. Vol. 15. No 2. pp. 28-34].
- Korzhevskii D.E., Sukhorukova E.G., Gilerovich E.G., Petrova E.S., Kirik O.V., Grigor`ev I.P. Advantages and disadvantages of zinc-ethanol-formaldehyde as a fixature for immunocytochemical studies and confocal laser microscopy // Neurosci. Behav. Physiol. 2014. Vol. 44, No 5. P. 542-545.
- Schaefer M.H., Wanker E.E., Andrade-Navarro M.A. Evolution and function of CAG/polyglutamine repeats in protein-protein interaction networks // Nucleic Acids Res. 2012. Vol. 40, No 10. P. 4273-4287.
- Freiman R.N., Tjian R. Neurodegeneration. A glutamine-rich trail leads to transcription factors // Science. 2002. Vol. 296, No 5576. P. 2149-2150.
- Cattaneo E., Zuccato C., Tartari M. Normal huntingtin function: an alternative approach to Huntington's disease // Nat. Rev. Neurosci. 2005. Vol. 6, No 12. Р. 919-930.
- Rigamonti D., Sipione S., Goffredo D., Zuccato C., Fossale E., Cattaneo E. Huntingtin's neuroprotective activity occurs via inhibition of procaspase-9 processing // J. Biol. Chem. 2001. Vol. 276. No 18. Р. 14545-14548.
- Korzhevskii D.E., Grigor`ev I.P., Kirik O.V., Alekseeva O.S. Neuroglobin distribution in the rat cerebellar Purkinje cells // J. Evol. Biochem. Physiol. 2015. Vol. 51, No 6. P. 517-519.
- Kirik O.V., Grigorev I.P., Alekseeva O.S., Korzhevskii D.E. Three-Dimensional Organization of the Cytoplasmic Neuroglobin-Immunopositive Structures in the Rat Medulla Oblongata Neurons // Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology. 2016. Vol. 10, No 4. P. 333-337.
Supplementary files
![](/img/style/loading.gif)