The role of hypoxia in the integrity of the genetic apparatus and the formation of memory in drosophila in the paradigm of conditioned reflex suppression of courtship

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Abstract

M.E. Lobashev and V.В. Savvateev in 1959 obtained unique data on the expansion of the adaptive capabilities of the organism when training the properties of higher nervous activity by the formation of conditioned food reflexes to stimuli that exhaust the nervous system. Apparently, the formation of a conditioned connection help to overcoming stressful effects, adaptation to restrictive conditions, and changes in the functioning of the nervous system. To test this assumption, the influence of stressful influences hypoxia on learning and memory of Drosophila in the paradigm of conditioned reflex suppression of courtship was studied. The results were obtained on the enhancement of the ability to learn under hypoxic exposure. These experimental conditions did not affect memory formation. The effect of hypoxia on chromosomes through the formation of double-stranded breaks was revealed. The data are discussed in light of the relationship between neuroplasticity processes and mechanisms of adaptation to stressors.

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About the authors

Anna V. Medvedeva

Pavlov Institute of Physiology RAS

Author for correspondence.
Email: avmed56@mail.ru
Scopus Author ID: 16689705800

PhD, Senior Researcher of Laboratory of Neurogenetics

Russian Federation, Saint Petersburg

Elena V. Tokmacheva

Pavlov Institute of Physiology RAS

Email: tokmatcheva@mail.ru

PhD, Researcher of Laboratory of Neurogenetics

Russian Federation, Saint Petersburg

Ekaterina A. Nikitina

Pavlov Institute of Physiology RAS; Herzen State Pedagogical University

Email: 21074@mail.ru
ORCID iD: 0000-0003-1897-8392
SPIN-code: 7844-8621
Scopus Author ID: 56603106300

DrSc, Head of Department of Human and Animal Anatomy and Physiology; Leader Researcher of Laboratory of Neurogenetics

Russian Federation, Saint Petersburg

Svetlana Alexandrovna Vasilieva

Herzen State Pedagogical University

Email: swetlana.gorohowa@yandex.ru
ORCID iD: 0000-0002-7785-7091

Assistent of Department of Human and Animal Anatomy and Physiology

Russian Federation, Saint Petersburg

Ekaterina S. Zalomaeva

Pavlov Institute of Physiology RAS; Herzen State Pedagogical University

Email: Zalomaeva.E@yandex.ru
ORCID iD: 0000-0002-6005-3433
SPIN-code: 2075-1823

Assistent of Department of Human and Animal Anatomy and Physiology; Post-graduate student of Laboratory of Neurogenetics

Russian Federation, Saint Petersburg

Elena V. Savvateeva-Popova

Pavlov Institute of Physiology RAS

Email: esavvateeva@mail.ru
SPIN-code: 2559-4778
Scopus Author ID: 6603078303

DrSc, Head of Laboratory of Neurogenetics

Russian Federation, Saint Petersburg

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2. Fig. 1. Frequency of chromosome rearrangements after hypoxia exposure at different time intervals. CS — wild-type Drosophila line Canton-S. * statistically significant differences from control, p ≤ 0.05.

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3. Fig. 2. Effects of hypoxia on sexual behavior elements of untrained Drosophila males. UWH — untrained without hypoxia; U1HH — untrained within 1 hour after hypoxia; U3HH — unearthed 3 hours after hypoxia. * statistically significant differences from control, p ≤ 0.05

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4. Fig. 3. Effect of hypoxia training on sexual behavior components in Drosophila in the conditioned courtship suppression paradigm. U1HH — untrained within 1 hour after hypoxia; THC0 — males training in hypoxic conditions immediately after 30 min of training in the barocamera. * statistically significant differences from control, p ≤ 0.05

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5. Fig. 4. Effect of hypoxia on Drosophila learning and memory in the conditioned courtship suppression paradigm. C0 — control males without hypoxia — test immediately after training (learning); THC0 — males training in hypoxic conditions immediately after 30 min of training in the barocamera; C3 — control males without hypoxia — 3 hours after training (memory); THC3 — males training in hypoxic conditions 3 hours after training. * statistically significant differences from control, p ≤ 0.05; • statistically significant differences from males tested immediately after training, p ≤ 0.05

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Copyright (c) 2021 Medvedeva A.V., Tokmacheva E.V., Nikitina E.A., Vasilieva S.A., Zalomaeva E.S., Savvateeva-Popova E.V.

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