LPS-INDUCED GENE EXPRESSION CHANGES OREXIN RECEPTOR TYPES I AND II (AND OxR1 OxR2) IN CELLS OF THE CENTRAL NERVOUS SYSTEM



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Abstract

The article is devoted to the dynamic of gene expression orexin receptor in cells of the structures brain and spinal cord in the first hours after the application of antigen. First shows the change of dynamic of gene expression of receptors for orexin in the first hours after the introduction of the T-independent antigen - lipopolysaccharide, which is reflected in the increase in the concentration of mRNA OхR1 and OхR2 in the mesencephalon cells, and only mRNA of OхR1 in the thoracic segments of the spinal cord cells. Has been defined reduction of gene expression of a OxR2 in the hypothalamus, which indicates the difference vector changes its orexin sensitive cells of various structures of the brain and possibly their intensity realization of ligand-receptor interactions, orexin A and B. The most expressed changes of level of an expression of genes of receptors to orexin are found in cases of application of an anti-gene of small doses.

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About the authors

N S Novikova

Institute of Experimental Medicine of the North West Branch of the Russian Academy of Medical Science

Email: novikiem@gmail.com

S V Perekrest

Institute of Experimental Medicine of the North West Branch of the Russian Academy of Medical Science

Email: perekrest.sv@gmail.com

K Z Shainidze

Institute of Experimental Medicine of the North West Branch of the Russian Academy of Medical Science

V A Masina

Institute of Experimental Medicine of the North West Branch of the Russian Academy of Medical Science

A D Steinzeig

Institute of Experimental Medicine of the North West Branch of the Russian Academy of Medical Science

E A Korneva

Institute of Experimental Medicine of the North West Branch of the Russian Academy of Medical Science

academician RAS

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Copyright (c) 2014 Novikova N.S., Perekrest S.V., Shainidze K.Z., Masina V.A., Steinzeig A.D., Korneva E.A.

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