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Design of ELISA test-kits for organ specific autoantibodies determination on the base of microsome fractions of adrenal and gonads steroid producing cells
- Authors: Khokhlov P.P.1, Shabanov P.D.1
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Affiliations:
- Institute of Experimental Medicine NWB RAMS
- Issue: Vol 10, No 4 (2012)
- Pages: 83-90
- Section: Articles
- URL: https://journals.eco-vector.com/RCF/article/view/756
- DOI: https://doi.org/10.17816/RCF10483-90
- ID: 756
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Abstract
The aim of this work was to design ELISA test-kits for quantitative measurement of autoantibodies to immune reactive structures of human steroid-producing cells. The antigens were immobilized in solid phase including microsome fractions of human adrenals, Leidig male cells and female ovarian granulose cells. The use of biopsy of human adrenal and gonad material, standardized antibodies fractions of patients with adrenal or gonad autoimmune lesion, have permitted to design ELISA test-kits. That test-kits have high sensibility and specificity to organ-specific autoantibodies for autoimmune diseases. These test-kits on the bases of microsome fractions of human adrenals and gonads as immobilized antigens have sensibility, accuracy and precision needed for such routine ELISA kits.
About the authors
Platon Platonovich Khokhlov
Institute of Experimental Medicine NWB RAMS
Email: platonkh@list.ru
PhD (Biochemistry), Researcher, S. V. Anichkov Dept. of NeuroPharmacology
Petr Dmitriyevich Shabanov
Institute of Experimental Medicine NWB RAMS
Email: pdshabanov@mail.ru
Dr. Med. Sci. (Pharmacology), Professor, Head, Dept. of Neuropharmacology
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