The influence of experimentally changedthyroid status on cognitive activity and angiogenesis in the brain of female inbred С3Н-А mice



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Abstract

The study was performed using 33 virgin females of inbred C3H-A mice. The animals were randomized as follows 3 : 2 : 2. The first group was hyperthyroid (n1 = 15), the second hypothyroid (n2 = 10) and the third euthyroid (control) (n3 = 8). The results of individual behavior investigation as to 18 and 40 weeks of our experiment in “open field” test clarified that quantitative traces of almost whole components of cognitive activity was much higher in hyperthyroid mice as compared to other groups (control and hypothyroid ones). The cognitive behavior activity has gradually been decreasing in hypothyroid group especially in connection to burrow reflex. As to those elements of behavior which are of characteristics of emotions the results were a bit different. The emotional characteristics were more expressed in hyperthyroid group as compared to control and hypothyroid ones. The immunohistochemical investigations clearly showed that expression of vascular endothelial growth factor (VEGF) in neocortex and hyppocampus prevails in hyperthyroid mice and less expressed in animals deprived of thyroid hormones (p < 0.05). The level of glial fibrillar acidic protein (GFAP) expression was significantly lower in brain of hypothyroid animals (p < 0.05). Otherwise the level of platelet derived growth factor receptor-α (PDGF-Rα) expression was much higher in brain of hypothyroid mice (p < 0.05). Our results presented for this paper confirmed the key role of thyroid hormones in regulation of cell interaction not only for developing neural cells but also for adult central nerve system.

About the authors

Ruslan Ivanovich Glushakov

State Pediatric Medical University of St.Petersburg

Email: glushakovruslan@gmail.com
PhD, Oncologist

Yelena Vasilyevna Kozyrko

Kirov Military Medical Academy, Ministry of Defense of the Russian Federation

student

Gennadiy Aleksandrovich Raskin

State Pediatric Medical University of St.Petersburg

Patologist

Inessa Vladimirovna Karpova

Pavlov State Medical University of St. Petersburg

Email: inessa.karpova@gmail.com
PhD, Dozent, Dept. of Normal Physiology

Andrey Andreyevich Lebedev

Institute of Experimental Medicine, NWB RAMS

Dr. Biol. Sci., Professor, Leading Researcher, Anichkov Dept. of Neuropharmacology

Vladimir Vladimirovich Mikheyev

Kirov Military Medical Academy, Ministry of Defense of the Russian Federation

Dr. Biol. Sci., Assistant Professor, dept. of Pharmacology

Natalya Igorevna Tapilskaya

State Pediatric Medical University of St.Petersburg

Dr. Med. Sci., Professor, Dept. of Obstetrics and Gynecology

Sergey Nikolayevich Proshin

Mechnikov North-West State Medical University of St. Petersburg, Ministry of Health Care of the Russian Federation

Email: psnjsn@rambler.ru
Dr. Med. Sci., Docent, Dept. of Pharmacology

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Copyright (c) 2013 Glushakov R.I., Kozyrko Y.V., Raskin G.A., Karpova I.V., Lebedev A.A., Mikheyev V.V., Tapilskaya N.I., Proshin S.N.

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