The role of N-cholinoreceptors in somatovisceral intersystemic interactions in early rat ontogenesis

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Interaction of slow-wave rhythmic components of cardiac, respiratory and motor activities was analyzed in non-narcotized of newborn 1-day-old (P1) and 16-day-old (P16) Wistar rat pups under normal and impaired cholinergic regulation. Functional activity of these three systems is rhythmic, and coordination of their functioning is an important element of the mechanism of adaptive rearrangements under changing factors of the external and internal environment. The acetylcholinesterase inhibitor physostigmine (eserine) was used to increase the level of endogenous acetylcholine and enhance cholinergic effects. To reveal the role of N-cholinoreceptors in intersystemic somatovisceral interactions (ISI), we performed blockade of this receptor type with benzohexonium. Administration of physostigmine leads to the development of a number of pathological reactions and a decrease in the level of ISI in all ranges of modulating rhythms in rats of both ages. ISI in younger rats appear to be more resistant to changes in the level of cholinergic activation. Blockade of N-cholinoreceptors causes inhibition of ISI at P1 and partially to their potentialization at P16. The activation of cholinoreactive structures, which occurred against the background of cholinoreceptors blockade, reduces the pathological effects of physostigmine in animals of both ages, but at the same time leads to an attenuation of ISI. This weakening is more pronounced in 16-day old rats, which may indicate the formation of the definitive level of cholinergic regulation in the first weeks of postnatal ontogenesis.

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Vladimir A. Sizonov

Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences

Author for correspondence.

Senior Researcher

Russian Federation, 44 Toreza str., Saint Petersburg, 194223

Ludmila E. Dmitrieva

Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences

ORCID iD: 0000-0003-4744-5253
SPIN-code: 9608-4428

PhD (Physiology), Senior Researcher

Russian Federation, 44 Toreza str., Saint Petersburg, 194223

Sergey V. Kuznetsov

Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences

ORCID iD: 0000-0003-2614-2632
SPIN-code: 6663-0070

Dr. Biol. Sci. (Physiology) Chief Researcher

Russian Federation, 44 Toreza str., Saint Petersburg, 194223


  1. Coyle JT, Vamamura HI. Neurochemical aspects of the ontogenesis of cholinergic neurons in the rat brain. Brain Res. 1976;118(3):429–440. doi: 10.1016/0006-8993(76)90310-3
  2. Stashina EV, Gavrilov NA, Shabanov PD. Neurobehavioral effects of choninergic drugs in prenatal period. Reviews on Clinical Pharmacology and Drug Therapy. 2017;15(3):5–21. (In Russ.) doi: 10.17816/RCF1535-21
  3. Bairamov AA, Poletaeva AO, Efremov OM, et al. Neirokhimicheskie ehffekty prenatal’nogo vozdeistviya selektivnykh M- i N-kholinoblokatorov v rannem ontogeneze. Psychopharmacology and Biological Narcology. 2008;8(1-2-2):2352. (In Russ.)
  4. Dmitrieva LE, Sizonov VA, Kuznetsov SV. Izmenenie aktivnosti somato-vistseral’nykh sistem u novorozhdennykh krysyat posle vozdeistviya ingibitora kholinehsteraz ehzerina i premedikatsii N-kholinoblokatorami. 15-e Vserossiiskoe soveshchanie i 8-ya Shkola po ehvolyutsionnoi fiziologii. Sbornik materialov. Saint Petersburg: 2016. Р. 69–70. (In Russ.)
  5. Sizonov VA, Dmitrieva LE. Heart rhythm disturbances caused by injection of cholinesterase inhibitor physostigmine to rats during the early ontogeny. Bull Experimental Biol and Med. 2018;165(1):44–47. doi: 10.1007/s10517-018-4095-9
  6. Sizonov VA, Dmitrieva LE. Changes in activities of somatovisceral systems in newborn rats under conditions of nicotinic cholinoreceptor blockage and activation of cholinoreactive structures. Bull Experimental Biol and Med. 2019;167(2):220–225. doi: 10.1007/s10517-019-04495-z
  7. Rusinov VS, Grindel’ OM. Biopotentsialy mozga cheloveka. Moscow: Meditsina, 1987. 253 p. (In Russ.)
  8. Sizonov VA, Dmitrieva LE, Kuznetsov SV. Somatovisceral intersystemic interactions in newborn rats upon effect of blockade of M-cholinoreceptors and activation of cholinoreactive structures. J Evol Biochem Physiol. 2020;56(3):348–363. doi: 10.1134/S0022093020040067
  9. Kuznetsov SV. Character of viscero- and somatomotor interactions in rat pups at changes of the level of activity of adrenergic structures. J Evol Biochem Physiol. 2004;40(3):293–306. doi: 10.1023/B: JOEY.0000042634.71156.a7
  10. Bursian AV, Dmitrieva LE, Sizonov VA. Secondary rhythms of automatically functioning systems. J Evol Biochem Physiol. 2009;45(6):670–677. doi: 10.1134/S0022093009060040
  11. Kuznetsov SV, Dmitrieva LE, Sizonov VA. Cardiac, respiratory, and motor activity in norm and after activation of catecholaminergic systems in newborn rat pups. J Evol Biochem Physiol. 2012;48(4):430–444. doi: 10.1134/S002209301204007X
  12. Kuznetsov SV. O vozmozhnykh mekhanizmakh uchastiya tsentral’nykh kholinoreaktivnykh sistem v regulyatsii spontannoi motornoi aktivnosti v rannem ontogeneze krys. Journal of Evolutionary Biochemistry and Physiology 1994;30(2):217–224. (In Russ.)

Copyright (c) 2021 Sizonov V.A., Dmitrieva L.E., Kuznetsov S.V.

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