Search for SNP markers of stress tolerance in the TaDREB1 and TaWRKY19 genes of bread wheat in the Cis-Ural steppe zone

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Abstract

BACKGROUND: Under the conditions of the Cis-Ural steppe zone, abiotic stress factors such as winter conditions and drought have a negative impact on the yield of bread wheat (Triticum aestivum L.). However, the efficiency of breeding to these stress factors is limited by wide variations depending on the weather conditions in different years. To collect stress-resistant forms in the selected material, genomic selection can be used, however, SNP markers of drought tolerance and winter hardiness of bread wheat, unique to this region, are unknown.

AIM: The purpose of this study is to identify SNP markers in the genes of the key transcription factors of bread wheat TaDREB1 and TaWRKY19, which are involved in the regulation of plant resistance to abiotic stresses.

MATERIALS AND METHODS: 16 varieties and lines of bread wheat were analyzed, contrasting in terms of winter hardiness and drought tolerance. DNA from wheat leaves was isolated by the standard CTAB method. Primers were selected, fragments of open reading frames of the studied genes were amplified, and nucleotide sequences were determined by automatic capillary sequencing.

RESULTS: Among the analyzed bread wheat varieties and lines, 12 SNPs in the TaDREB1 gene and 5 SNPs in TaWRKY19 were found.

CONCLUSIONS: The results of statistical analysis showed that substitution 866 T/A in the TaDREB1 gene is associated with winter hardiness, while substitution 587 A/G in the TaWRKY19 gene is associated with winter hardiness and drought resistance.

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About the authors

Evgeniya A. Zaikina

Institute of Biochemistry and Genetics, Ufa Federal Research Center Russian Academy of Sciences

Author for correspondence.
Email: evisheva@yandex.ru
ORCID iD: 0000-0003-1070-0804
SPIN-code: 4224-0089
Scopus Author ID: 49664463200

Cand. Sci. (Biol.), Research Associate of the Laboratory of Plant Genomics

Russian Federation, Ufa

Kamil R. Ismagilov

Bashkir Scientific Research Institute of Agriculture, Ufa Federal Research Center Russian Academy of Sciences

Email: ismagilovkr@gmail.com
SPIN-code: 6896-8797

Cand. Sci. (Econ.), Head of the Laboratory of selection and seed production of spring wheat

Russian Federation, Ufa

Bulat R. Kuluev

Institute of Biochemistry and Genetics, Ufa Federal Research Center Russian Academy of Sciences; N.I. Vavilov All-Russian Institute of Plant Genetic Resources

Email: kuluev@bk.ru
ORCID iD: 0000-0002-1564-164X
SPIN-code: 8580-5347
Scopus Author ID: 23094029400

Dr. Sci. (Biol.), Head of the Laboratory of Plant Genomics

Russian Federation, Ufa; Saint Petersburg

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Supplementary files

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2. Fig. 1. Alignment results of the TaDREB1 gene fragment in the examined samples of soft wheat. The AF303376.1 sequence from GenBank was used as a reference gene. The DREB_1_pr-DREB_16_p nucleotide sequences correspond to the studied varieties and lines 1-16 from Table 1. Nucleotides were numbered according to the reference gene from GenBank

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3. Fig. 2. Results of nucleotide sequence alignment of the homeologous TaDREB1 genes (3A, 3B, 3D). Nucleotide numbering was performed according to the reference gene from GenBank

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4. Fig. 3. Alignment results of the TaWRKY19 gene fragment in the examined samples of soft wheat. The EU665430.1 sequence from GenBank was used as a reference gene. The WRKY_1_pr-WRKY_16_p nucleotide sequences correspond to the studied varieties and lines 1-16 from Table 1. Nucleotides were numbered according to the reference gene from GenBank

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5. Fig. 4. Results of nucleotide sequence alignment of the homeologous TaWRKY19 genes (2A, 2B, 2D). Nucleotide numbering was performed according to the reference gene from GenBank

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