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The use of R-nj, B1, Pl1 genes to improve marker properties in the selection of maize haploinducers
- Authors: Liu X.1, Ulyanov A.V.2, Khatefov E.B.2
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Affiliations:
- Maize Research Institute, Liaoning Academy of Agricultural Sciences
- N.I. Vavilov All-Russian Institute of Plant Genetic Resources
- Issue: Vol 20, No 3 (2022)
- Pages: 193-202
- Section: Genetic basis of ecosystems evolution
- URL: https://journals.eco-vector.com/ecolgenet/article/view/108374
- DOI: https://doi.org/10.17816/ecogen108374
- ID: 108374
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Abstract
BACKGROUND: Studies were carried out on the creation of haploinductor lines using various methods for testing haploinductor and marker ability from the VIR maize genetic collection, adapted to the conditions of the Liaoning province in China and the South of Russia.
MATERIALS AND METHODS: On 22 maize testers, studies were carried out on the creation of haploinductor lines and testing the haploinductor and marker ability by the test-cross method. Sources of R1-nj, B1, Pl1 genes from the VIR maize genetic collection were used.
RESULTS: The lines of maize haploinducers Liaoyu No. 1, Liaoyu No. 2, Liaoyu No. 3 and Liaoyu No. 4 were created with a haploinduction frequency of 6.85%, 7.53%, 6.66% and 6.03%. respectively, with a high quality of labeling with the R1-nj, B1, Pl1 genes in test crosses.
CONCLUSIONS: In the process of selection of haploid maize on the basis of genes A, haploinductor lines with a high frequency of haploinduction and high efficiency of markers in test crosses, abundant pollen and grain productivity, resistance to late blight and lodging, and adaptation to the agroclimatic zone of Shenyang Province (China) were obtained and the North Caucasian Federal District (Russia).
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About the authors
Xinfang Liu
Maize Research Institute, Liaoning Academy of Agricultural Sciences
Email: xinfangliu2002@126.com
Master, Junior Research Associate
Taiwan, Province of China, Shenyang, LiaoningAlexey V. Ulyanov
N.I. Vavilov All-Russian Institute of Plant Genetic Resources
Email: krasnixin56@gmail.com
Research Laboratory Assistant
Russian Federation, Saint PetersburgEduard B. Khatefov
N.I. Vavilov All-Russian Institute of Plant Genetic Resources
Author for correspondence.
Email: haed1967@rambler.ru
ORCID iD: 0000-0001-5713-2328
SPIN-code: 7929-8148
Dr. Sci. (Biol.), Leading Research Associate
Russian Federation, Saint PetersburgReferences
- Chase SS. Monoploid frequencies in a commercial double cross hybrid maize, and its component single cross hybrids and inbred lines. Genetics. 1949;34(1):328–332. doi: 10.1093/genetics/34.3.328
- Asadova GM, Ulyanov AV, Karlov AV, Khatefov EB. The prospects for using haploinducers in maize breeding. Plant Biotechnology and Breeding. 2020;3(2):16–29. (In Russ.) doi: 10.30901/2658-6266-2020-2-o3
- Mayor P, Bernardo R. Doubled haploids in commercial maize breeding: one-stage and two-stage selection versus marker-assisted recurrent selection. Maydica. 2007;54:439–448.
- Shatskaya OA. Haploinductors isolation in maize: three cycles of selection on high frequency of induction of matroclinal haploids. Agricultural biology. 2010;45(5):79–86. (In Russ.)
- Khokhlov SS, Tyrnov VS, Grishina EV, Davoyan NI. Gaploidiya i selektsiya. Moscow: Nauka, 1976. 221 p. (In Russ.)
- Tyrnov VS, Zavalishina AN. Inducing high frequency of matroclinal haploids in maize. Doklady Akademii Nauk. 1984;276(3):735–738. (In Russ.)
- Coe EH. A line of maize with high haploid frequency. Am Nat. 1959;XCIII(873):381–382. doi: 10.1086/282098
- Chumak MV. The use of haploid in breeding maize. Proceedings of the tenth meeting of the maize and sorghum section of EUCARPIA; 1979 Sept 17–19; Varna.
- Zavalishina AN, Tyrnov VS. Induction of matroclinal haploidy in maize in vivo. XIII Eucarpia Congress, Reproductive Biology and Plant Breeding. Angers, France. 1992. P. 221–222.
- Rotarenco V, Dicu G, State D, Fuia S. New inducers of maternal haploids in maize. Maize Genet Coop Newsletter. 2010;84:21–22.
- Prigge V, Schipprack W, Mahuku G, et al. Development of in vivo haploid inducers for tropical maize breeding programs. Euphytica. 2012;185:481–490. doi: 10.1007/s10681-012-0657-5
- Xu X, Li L, Dong X, et al. Gametophytic and zygotic selection leads to segregation distortion through in vivo induction of a maternal aploid in maize. J Exp Bot. 2013;64(4):1083–1096. doi: 10.1093/jxb/ers393
- Liu ZZ, Song TM. Breeding and identification of high frequency parthenogenetic haploid induction lines in maize. Acta Agronomica Sinica. 2000;26(5):570–574. (In Chinese).
- Cai Z, Xu GL, Liu XH, et al. The breeding of JAAS3-Haploid inducer with high frequency parthenogenesis in maize. Journal of Maize Sciences. 2007;15(1):1–4. (In Chinese).
- Yue YH, Lu M, Zhang JX, et al. Breeding of maize haploid high frequency induction line Jiyou. Crop. 2017;101(3):35–38. (In Chinese).
- Röber FK, Gordillo GA, Geiger HH. In vivo haploid induction in maize — performance of new inducers and significance of doubled haploid lines in hybrid breeding. Maydica. 2005. Vol. 50. P. 275–283.
- Chen S, Song T. Identification haploid with high oil xenia effect in maize. Acta Agronomica Sinica. 2003;29:587–590.
- Lasharmes P, Bekert M. Genetic control of maternal haploidy in maize (Zea mays L.) and selection of haploid inducing lines. Theoretical and Applied Genetics. 1988;76:405–410. doi: 10.1007/BF00265341
- Chalyk S.T. Properties of maternal haploid maize plants and potential application to maize breeding. Euphytica. 1994;79:13–18.
- Melchinger AE, Schipprack W, Würschum T, et al. Rapid and accurate identification of in vivo-induced haploid seeds based on oil content in maize. Sci. Rep. 2013;3:2129.
- Gayen P, Madan JK, Kumar R, Sarkar KR. Chromosome doubling in haploids through colchicine. Maize Genet. Coop. Newslett. 1994;68:65.
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