Modern approach of structuring the variety diversity of the naked and covered forms of cultural oats (Avena sativa L.)

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  • Authors: Loskutov I.G.1,2, Shelenga T.V.1, Konarev A.V.1, Vargach Y.I.3, Porokhovinova E.A.4, Blinova E.V.1, Gnutikov A.A.1, Rodionov A.V.5,2
  • Affiliations:
    1. Federal Research Center “N.I. Vavilov All-Russian Institute of Plant Genetic Resources”
    2. St. Petersburg State University
    3. All-Russian Institute of Horticulture and Nursery
    4. Federal Research Center N.I. Vavilov Institute of Plant Genetic Resources (VIR)
    5. V.L. Komarov Botanical Institute RAS
  • Issue: Vol 18, No 1 (2020)
  • Pages: 27-41
  • Section: 1. Genetic basis of ecosystems evolution
  • URL: https://journals.eco-vector.com/ecolgenet/article/view/12977
  • DOI: https://doi.org/10.17816/ecogen12977
  • Cite item
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Abstract


Structuring and phenotyping genetic diversity is an important aspect of the work with breeding sources and materials.

In the Introduction, the authors pointed out the role of N.I. Vavilov’s scientific foresight in defining the topical trend in researching the genetic diversity of a crop, particularly the analysis of its biochemical composition. As the target of their research, the authors chose biochemical characters identifiable in the process of metabolomic analysis conducted by means of gas chromatography with mass spectrometry.

 Materials and methods. The object was the grain of naked and covered forms of common oat (Avena sativa L.) from the collection held by the Oat, Rye and Barley Genetic Resources Department of VIR. The analysis of oil fatty acid content and metabolomic research were performed using the method of gas chromatography with mass spectrometry on the chromatograph Agilent 6850 (USA).

Results. The obtained metabolomic spectra which reflected the metabolomic status of genotypes of various ecogeographic origin were compared among themselves using statistical (principal component) analysis methods. The results of the comparison are discussed by referring to the most important groups of metabolites significant for forming the traits of resistance to stressors as well as the characters related to food qualities of grain products. Special attention has been paid to biologically active compounds determining the functional value of the products for human nutrition: the sum of phenolics in covered forms is five times higher than that in naked ones and the content of glycine in covered forms is five times higher than in naked grain, with a similar proportion in the content of organic acids, sugars, etc.

Conclusion. Differences between metabolomic profiles of naked and covered forms have been detected and statistically verified. Accessions with the most optimal nutritional composition have been identified for food purposes and for the development of resistance to biotic and abiotic environmental stresses.


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About the authors

Igor G. Loskutov

Federal Research Center “N.I. Vavilov All-Russian Institute of Plant Genetic Resources”; St. Petersburg State University

Author for correspondence.
Email: i.loskutov@vir.nw.ru
ORCID iD: 0000-0002-9250-7225
SPIN-code: 2715-2082
Scopus Author ID: 8619012600
ResearcherId: D-5238-2013

Russian Federation, Saint Petersburg

Doctor of Biological Sciences, Chief Researcher, Acting Head of the Department of Genetic Resources of Oats, Rye, Barley; Professor, Department of Agrochemistry

Tatyana V. Shelenga

Federal Research Center “N.I. Vavilov All-Russian Institute of Plant Genetic Resources”

Email: tatianashelenga@yandex.ru

Russian Federation, Saint Petersburg

Candidate of Biological Sciences, Leading Researcher of the Department of Biochemistry and Molecular Biology

Alexey V. Konarev

Federal Research Center “N.I. Vavilov All-Russian Institute of Plant Genetic Resources”

Email: a.konarev@vir.nw.ru

Russian Federation, Saint Petersburg

Doctor of Biological Sciences, Professor, Acting Head of the Department of Biochemistry and Molecular Biology

Yulia I. Vargach

All-Russian Institute of Horticulture and Nursery

Email: ulvargach@gmail.com

Russian Federation, Moscow

Candidate of Agricultural Sciences, Junior Researcher, Gene Pool Department

Elizaveta A. Porokhovinova

Federal Research Center N.I. Vavilov Institute of Plant Genetic Resources (VIR)

Email: e.porohovinova@mail.ru
SPIN-code: 5033-3263

Russian Federation, Saint Petersburg

Candidate of Biological Sciences, Senior Researcher, Department of Genetic Resources of Oilseeds and Spinning Crops

Elena V. Blinova

Federal Research Center “N.I. Vavilov All-Russian Institute of Plant Genetic Resources”

Email: i.loskutov@vir.nw.ru

Russian Federation, Saint Petersburg

Candidate of Agricultural Sciences, Senior Researcher, Department of Genetic Resources of Oats, Rye, Barley

Alexander A. Gnutikov

Federal Research Center “N.I. Vavilov All-Russian Institute of Plant Genetic Resources”

Email: alexandr2911@yandex.ru

Russian Federation, Saint Petersburg

Candidate of Agricultural Sciences, Senior Researcher, Department of Genetic Resources of Oats, Rye, Barley

Alexander V. Rodionov

V.L. Komarov Botanical Institute RAS; St. Petersburg State University

Email: avrodionov@mail.ru

Russian Federation, Saint Petersburg

Professor, Chief Researcher, Acting Head of the Laboratory of Biosystematics and Cytology; Professor

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Supplementary files

Supplementary Files Action
1.
Fig. 1. The main groups of metabolites of grains of membranous and glacial oats as a percentage (± 0.95 confidence interval) of the total content of all identified substances

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2.
Fig. 2. The distribution of the studied compounds and oat samples in the system of two factors: a - substances, factors 1 and 2; b - samples, factors 1 and 2; c - substances, factors 3 and 4; d - samples, factors 3 and 4

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3.
Fig. 3. The number of compounds characteristic of the metabolite profiles of the kernels of the holoserous and membranous forms of Avena sativa L.

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Copyright (c) 2020 Loskutov I.G., Shelenga T.V., Konarev A.V., Vargach Y.I., Porokhovinova E.A., Blinova E.V., Gnutikov A.A., Rodionov A.V.

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