Manifestations of resistance to acaricides in inbred lines of two-spotted spider mite in the process of disruptive selection

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Abstract


Background: Information about genetic and biochemical mechanisms of arthropods resistance to pesticide obtained with resistant and susceptible genotypes is more correct than that of population-based samples. Materials and methods: The disruptively selection of two-spotted spider mites was carried out on the basis of the presence or absence of resistance to four acaricides - dimethoate, bifenthrin, abamectin and bromopropylate. A possible resistance mechanisms of mite to abamectin and bromopropylate was detected when testing the diagnostic` concentrations of acaricides which toxic action is known. Synergetic effect of resistant genotypes treated with monooxygenase inhibitor piperonyl butoxide (PBO) was studied. Carboxylesterase isoenzymes were determined with use of poliacrylamide gel electrophoresis in individual genotypes of spider mite. Results: Statistical analysis of genotypes selection results demonstrated 30 % of females with no traits of resistance to the toxicant in all resistant lines of each generation. The reason this is arrhenotokous reproduction spider mites and dominant status of alleles determining the traits of resistance. Abamectin and bromopropylate resistant mites were synergized by PBO. Resistance was positively correlated with increased carboxylesterase activity in resistant genotypes. Conclusion: Reduced sensitivity to pesticides in resistant arthropods was found in alteration recordings sensory receptivity and through elevated levels of carboxylesterase and monooxygenase activity. This is universal adaptive response of the arthropods organism to intoxication by any pesticides.

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About the authors

Oleg Veniaminovich Sundukov

All-Russian Institute of Plant Protection

Email: Sunduckov.oleg@yandex.ru
PhD, Senior scientist, Laboratory ecotoxicology

Irina Anatolievna Tulaeva

All-Russian Institute of Plant Protection

Email: zubanov63@rambler.ru
PhD, scientist, Laboratory ecotoxicology

Evgeniy Aleksandrovich Zubanov

All-Russian Institute of Plant Protection

Email: zubanov63@rambler.ru
PhD, scientist, Laboratory ecotoxicology

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Copyright (c) 2015 Sundukov O.V., Tulaeva I.A., Zubanov E.A.

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