Cytogenetic analysis of residents of North Ossetia contacting with toxic factors

Cover Page
Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription or Fee Access


Background. The use of cytogenetic assays in the surveillance of populations occupationally exposed to a variety of xenobiotics originates from the assumption that chromosomal alterations might be causally involved in early stages of carcinogenesis. Materials and methods. This study was designed to assess genotoxic damage in somatic cells of workers contacting with harmful factors after the accident in metallurgical plant. The study was conducted on 29 workers (with the mean age of 44 ± 2.5) performing various jobs with harmful factors. Control group was recruited from occupationally unexposed individuals and comprised 18 persons with the mean age of 30 ± 2.2 and inplant control was 5 employees from metallurgical plant (accountants) with the mean age of 32 ± 5.9. Chromosome aberration analysis was performed on peripheral blood lymphocytes, cultures were incubated for 48h. Results. Among the residents with occupational hazards, a statistically significant increase of the frequency of the cells with chromosome aberrations in comparison with the control group (5.2 ± 0.3 and 2.4 ± 0.3, respectively). The highest frequency of cells with structural abnormalities was observed among steel plant workers (6.3 ± 0.6). Conclusion. Our findings suggest that in peripheral blood lymphocytes of residents contacting with harmful factors may induce clastogenic effects, indicating a potential health risk in populations of workers. A high average frequency of metaphases with aberrations and characterization of quality spectrum of cytogenetically violations is an indication of adverse environmental conditions which have developed after the accident.

Full Text

Restricted Access

About the authors

Fatima Taymurazovna Chshiyeva


Researcher, Central scientific laboratory


  1. Бочков Н. П. (1974) Метод учета хромосомных повреждений как биологический индикатор влияния факторов внешней среды на человека. М.: Наука.
  2. Бочков Н. П., Сапачева В. А., Филиппова Т. В. и др. (1993) Цитогенетическое обследование рабочих производства резины. Медицина труда и промышленная экология. № 5-6. С. 12-14.
  3. Бочков Н. П., Чеботарев А. Н. (1989) Наследственность человека и мутагены внешней среды. М.: Медицина.
  4. Дурнев А. Д., Середенин С. Б. (1998) Мутагены - скрининг и фармакологическая профилактика воздействий. М.: Медицина.
  5. Дудкина О. А., Минина В. И., Ларин С. А. и др. (2011) Канцерогены производственной среды и онкологическая заболеваемость на углеперерабатывающих производствах Кузбасса. Политравма. № 1. С. 91-97.
  6. Дружинин В. Г. (2003 а) Хромосомные нарушения у населения крупного промышленного региона: пространственно-временной цитогенетический мониторинг: Дисс… док. биол. наук. Москва.
  7. Дружинин В. Г. (2003 б) Количественные характеристики частоты хромосомных аберраций в группе жителей крупного промышленного региона Западной Сибири. Генетика. Т. 39: № 10. С. 1373-1380.
  8. Дружинин В. Г., Мокрушина Н. В., Минина В. И., Волков А. Н. (2003) Генотоксические эффекты у работников горно-обагатительного производства. Медицина труда и промышленная электроника. № 12. С. 16-20.
  9. Минина В. И., Дружинин В. Г., Глушков А. Н. и др. (2009) Количественные характеристики частоты хромосомных аберраций у жителей районов с различным уровнем онкологической заболеваемости. Генетика. Т. 45: № 2. С. 239-246.
  10. Савченко Я. А., Дружинин В. Г., Минина В. И. и др. (2008) Цитогенетический анализ генотоксических эффектов у работников теплоэнергетического производства. Генетика. Т. 44: № 6. С. 857-862.
  11. Battershill J. M., K. Burnett and S. Bull. (2008) Factors affecting the incidence of genotoxicity biomarkers in peripheral blood lymphocytes: impact on design of biomonitoring studies. Mutagenesis. Р. 1-15.
  12. Bonassi S., Hagman L., Stromberg U. et al. (2000) Chromosomal aberrations in lymphocytes predict human cancer independently of exposure to carcinogens. European Study Group on Cytogenetic Biomarkers and Health. Cancer Research. Vol. 60. P. 1619-1625.
  13. Garsia-Leston J, Romma-Torres J, Vilares M. et all (2012) Genotoxic effects of occupational exposure to lead and influence of polymorphisms in genes involved in lead toxicokinetiics and in DNA repair. Environment International. P. 29-36.
  14. Grover P, Rekhadevi PV, Danadevi K et all (2010) Genotoxicity evaluation in workers occupationally exposed to lead. Int. J. Hyg. Environ. Health. P. 99- 106.
  15. Hagmar L. et all and the European Study Group on Cytogenetic Biomarkers and Health (1998) Chromosomal Aberrations in Lymphocytes Predict Human Cancer: A Report from the European Study Group on Cytogenetic Biomarkers and Health (ESCH). Cancer Research. Vol. 58. P. 4117-4121.
  16. Fucic A., Znoar A., Strnad M. et all (2007) Chromosome damage and cancer risk in the workplace: the example of cytogenetic surveillance in Croatia. Toxicol Lett. Vol. 172 (1-2). P. 4-11.
  17. Rossner P. Jr., Rossnerova A., Sram R. J. (2011) Oxidative stress and chromosomal aberrations in an environmentally Exposed population. Mutation Research. Vol. 707. P. 34-41.
  18. Singh Z., Chadha P. and Sharma S. (2013) Evaluation of Oxidative Stress and Genotoxicity in Battery Manufacturing Workers Occupationally Exposed to Lead. Toxicol Int. P. 95-100.
  19. Sram R. J., Rossner P., Smerhovsky Z. (2004) Cytogenetic analysis and occupational health in the Czech Republic. Mutation Research. Vol. 566. P. 21-48.
  20. Smerhovskya Z., Landaa K., Rossner P. et al. (2002) Increased risk of cancer in radon-exposed miners with elevated frequency of chromosomal aberrations. Mutation Research. Vol. 514. P. 165-176.
  21. Musak L., Soucek P., Vodickova L. et al. (2008) Chromosomal aberrations in tire plant workers and interaction with polymorphisms of biotransformation and DNA repair genes. Mutation Research. Vol. 641 (1-2) P. 36-42.



Abstract - 434

PDF (Russian) - 215



Copyright (c) 2014 Chshiyeva F.T.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies