Reducing of LXRβ and PPARγ mRNA in M-CSF stimulated macrophages in patients with levels atherosclerosis

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Abstract


Nuclear receptors LXRα/β and PPARγ play an important role in lipid metabolism and reverse cholesterol transport. However, the influence of LXRα/β and PPARγ mRNA levels in macrophages on atherosclerosis remains unexplored. Using real time PCR, we determined LXRα mRNA, LXRβ mRNA and PPARγ mRNA levels in macrophages cultured for 5 days with macrophage colony-stimulating factor (M-CSF). Levels of LXRβ mRNA and PPARγ mRNA in patients with arterial stenosis were reduced when compared with the control group, p < 0.001. LXRa gene mRNA level in macrophages was not changed in the study groups, (p = 0.17). Thus, our study shows that the LXRβ and PPARγ genes expression levels in macrophages may be significant factors associated with the development of atherosclerosis.

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About the authors

Yekaterina Petrovna Demina

B. P. Konstantinov Nuclear Physics Institute in Saint-Petersburg

Email: citritt@gmail.com
Molecular and Radiation Biophysics Department

Valentina Vadimovna Miroshnikova

B. P. Konstantinov Nuclear Physics Institute in Saint-Petersburg

Email: mutantropol@mail.ru
Molecular and Radiation Biophysics Department

Nikolay Vladimirovich Mayorov

Pavlov First Saint-Petersburg State Medical University

Email: nikolaj-majorov@yandex.ru
Hospital Surgery Department N 2

Vladimir Valentinovich Davydenko

Pavlov First Saint-Petersburg State Medical University

Email: kuzet@mail.ru
Hospital Surgery Department N 2

Alexander L`vovich Schwarzman

B. P. Konstantinov Nuclear Physics Institute in Saint-Petersburg

Email: aschwart1@yandex.ru
Molecular and Radiation Biophysics Department

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Copyright (c) 2014 Demina Y.P., Miroshnikova V.V., Mayorov N.V., Davydenko V.V., Schwarzman A.L.

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