Influence of phytoecdysteroids and plants steroidal glycosides on the lifespan and stress resistance of drosophila melanogaster

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Abstract


Background. Elucidation of the molecular mechanisms of effects of the active substances of plant adaptogens is a topical area of researches. Materials and methods. We studied the effect of herbal substances containing phytoecdysteroids (20-hydroxyecdysone and inokosterone) of Serratula coronata L. or steroidal glycosides (dioscin and protodioscine) of Trigonella foenum-graecum L. on the expression level of stress response genes (genes of heat shock proteins, DNA repair, antioxidant defense and apoptosis), stressresistanse (paraquat, starvation, hyperthermia) and lifespan of Drosophila melanogaster. Results. The studied herbal substances upregulated genes of antioxidant defense mechanisms (Sod1), but downregulated the DNA repair (XPF and Rad51) and apoptosis (Hid) genes. At the same time herbal substances induced weak adaptogenic and antiaging effects. Conclusion. Our results demonstrate that the herbal substances containing phytoecdysteroids and steroidal glycosides change the expression level of stress-response genes and activate mechanisms of hormesis.

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About the authors

Mikhail Vyacheslavovich Shaposhnikov

Institute of Biology of the Komi Science Centre of the Ural Division RAS

Email: mshaposhnikov@mail.ru
Leading Researcher, PhD, Associate Professor, Laboratory of Molecular Radiobiology and Gerontology

Lyubov' Alekseevna Shilova

Institute of Biology of the Komi Science Centre of the Ural Division RAS

Email: lyubov.schilova@yandex.ru
assistant, Laboratory of Molecular Radiobiology and Gerontology

Ekaterina Nikolaevna Plyusnina

Institute of Biology of the Komi Science Centre of the Ural Division RAS

Email: kateplus@mail.ru
Researcher, PhD, Laboratory of Molecular Radiobiology and Gerontology

Svetlana Olegovna Volodina

Institute of Biology of the Komi Science Centre of the Ural Division RAS

Email: volodina@ib.komisc.ru
Head of Laboratory, Doctor of Biological Sciences, Professor, Laboratory of Biotechnology

Vladimir Vital'yevich Volodin

Institute of Biology of the Komi Science Centre of the Ural Division RAS

Email: volodin@presidium.komisc.ru
Head of Laboratory, Doctor of Biological Sciences, Professor, Laboratory of Biotechnology

Aleksey Aleksandrovich Moskalev

Institute of Biology of the Komi Science Centre of the Ural Division RAS

Email: amoskalev@list.ru
Head. Laboratory, Doctor of Biological Sciences, Associate Professor, Laboratory of Molecular Radiobiology and Gerontology

References

  1. Анисимов В. Н. (2003) Молекулярные и физиологические механизмы старения. СПб.: Наука.
  2. Белый В. А., Печникова А. А., Кочева Л. С., с соавт. (2010) Лигнины родиолы розовой и серпухи венценосной: особенности химической структуры и антиоксидантные свойства. Успехи геронтологии. Т. 23: (2): С. 221-227.
  3. Володин В. В., Матаев С. И. (2011) Экдистероидсодержащие растения - источники новых адаптогенов. Вестник биотехнологии и физико-химической биологии имени Ю. А. Овчинникова. Т. 7 (2): С. 52-59.
  4. Amrit F. R., Boehnisch C. M., May R. C. (2010) Phenotypic covariance of longevity, immunity and stress resistance in the Caenorhabditis nematodes. PLoS ONE. V. 5 (4): P. e9978.
  5. Arking R., Burde V., Graves K., et al. (2000) Forward and reverse selection for longevity in Drosophila is characterized by alteration of antioxidant gene expression and oxidative damage patterns. Exp. Gerontol. V. 35 (2): P. 167-185.
  6. Ashburner M. (1989) Drosophila: A laboratory manual. Cold Spring Harbor, N. Y.: Cold Spring Harbor Laboratory.
  7. Calabrese V., Cornelius C., Mancuso C., et al. (2008) Cellular stress response: a novel target for chemoprevention and nutritional neuroprotection in aging, neurodegenerative disorders and longevity. Neurochem. Res. V. 33 (12): P. 2444-2471.
  8. Danilov A., Shaposhnikov M., Plyusnina E., et al. (2013) Selective anticancer agents suppress aging in Drosophila. Oncotarget. V. 4 (9): P. 1527-1546.
  9. Le Bourg E. (2009) Hormesis, aging and longevity. Biochim Biophys Acta. V. 1790 (10): P. 1030-1039.
  10. Livak K. J., Schmittgen T. D. (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔC (T) Method. Methods. V. 25 (4): P. 402-408.
  11. Minois N. (2000) Longevity and aging: beneficial effects of exposure to mild stress. Biogerontology. V. 1 (1): P. 15-29.
  12. Moskalev A. (2007) Radiation-induced life span alteration of Drosophila lines with genotype differences. Biogerontology. V. 8 (5): P. 499-504.
  13. Moskalev A., Shaposhnikov M. (2011) Pharmacological inhibition of NF-κB prolongs lifespan of Drosophila melanogaster. Aging (Albany NY). V. 3 (4): P. 391-394.
  14. Moskalev A., Shaposhnikov M., Snezhkina A., et al. (2014) Mining gene expression data for pollutants (dioxin, toluene, formaldehyde) and low dose of gamma-irradiation. PLoS ONE. V. 9 (1): P. e86051.
  15. Moskalev A., Shaposhnikov M., Turysheva E. (2009) Life span alteration after irradiation in Drosophila melanogaster strains with mutations of Hsf and Hsps. Biogerontology. V. 10 (1): P. 3-11.
  16. Moskalev A. A., Plyusnina E. N., Shaposhnikov M. V. (2011) Radiation hormesis and radioadaptive response in Drosophila melanogaster flies with different genetic backgrounds: the role of cellular stress-resistance mechanisms. Biogerontology. V. 12 (3): P. 253-263.
  17. Moskalev A. A., Shaposhnikov M. V. (2010) Pharmacological inhibition of phosphoinositide 3 and TOR kinases improves survival of Drosophila melanogaster. Rejuvenation Res. V. 13 (2-3): P. 246-247.
  18. Mylnikov S. V., Kokko H. I., Karenlampi S. O., et al. (2005) Rubus fruit juices affect lipid peroxidation in a Drosophila melanogaster model in vivo. J. Agric. Food Chem. V. 53 (20): P. 7728-7733.
  19. Panossian A., Wikman G., Wagner H. (1999) Plant adaptogens. III. Earlier and more recent aspects and concepts on their mode of action. Phytomedicine. V. 6 (4): P. 287-300.
  20. Rattan S. I. (2008) Hormesis in aging. Ageing Res. Rev. V. 7 (1): P. 63-78.
  21. RCoreTeam. R: A Language and Environment for Statistical Computing. Cited 4.08.2014. URL: http://www.R-project.org.
  22. Shaposhnikov M., Latkin D., Plyusnina E., et al. (2014) The effects of pectins on life span and stress resistance in Drosophila melanogaster. Biogerontology. V. 15 (2): P. 113-127.
  23. Tatar M., Khazaeli A. A., Curtsinger J. W. (1997) Chaperoning extended life. Nature. V. 390 (6655): P. 30.
  24. Wang C., Li Q., Redden D. T., et al. (2004) Statistical methods for testing effects on “maximum lifespan”. Mech. Ageing Dev. V. 125 (9): P. 629-632.
  25. Yang P., He X. Q., Peng L., et al. (2007) The role of oxidative stress in hormesis induced by sodium arsenite in human embryo lung fibroblast (HELF) cellular proliferation model. Journal of toxicology and environmental health. Part A. V. 70 (11): P. 976-983.
  26. Zhao Y., Sun H., Lu J., et al. (2005) Lifespan extension and elevated hsp gene expression in Drosophila caused by histone deacetylase inhibitors. J. Exp. Biol. V. 208 (Pt 4): P. 697-705.

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Copyright (c) 2014 Shaposhnikov M.V., Shilova L.A., Plyusnina E.N., Volodina S.O., Volodin V.V., Moskalev A.A.

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