Comparative cytochemical analysis of hydrogen peroxide distribution in pea ineffective mutant SGEFix--1 (sym40) and initial line SGE



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Abstract

Comparative cytochemical analysis has revealed differences in hydrogen peroxide distribution in symbiotic nodules of pea initial line SGE and mutant SGEFix--1 (sym40). In the initial line SGE, precipitates of cerium perhydroxide were deposited in the walls of infection threads and in adjacent material in the luminal matrix. In mutant SGEFix--1, an increased deposition of cerium perhydroxide precipitates was observed in the matrix of hypertrophied infection droplets, round bacteria contained in infection threads and also around juvenile bacteroids. The observed pattern of hydrogen peroxide distribution indicates that bacteria in infected cells of mutant nodules are exposed to a stronger oxidative stress compared with nodules of the initial line.

About the authors

Anna V Tsyganova

All-Russia Research Institute for Agricultural Microbiology, Saint-Petersburg, RF

Email: anna_khodorenko@arriam.spb.ru Podbelskiy Ch., 3, Saint-Petersburg, Pushkin-8, 196608, Russ

Viktor E Tsyganov

All-Russia Research Institute for Agricultural Microbiology, Saint-Petersburg, RF

Email: viktor_tsyganov@arriam.spb.ru

Aleksey U Borisov

All-Russia Research Institute for Agricultural Microbiology, Saint-Petersburg, RF

Email: ayborisov@yandex.ru

Igor A Tikhonovich

All-Russia Research Institute for Agricultural Microbiology, Saint-Petersburg, RF

Email: arriam@arriam.spb.ru. contact@arriam.spb.ru Podbelskiy Ch., 3, Saint-Petersburg, Pushkin-8

Nicholas Brewin

The John Innes Centre, Norwich, UK

Email: nick.brewin@bbsrc.ac.uk NR4 7UH, Colney Lane, Norwich, United Kingdom

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Copyright (c) 2009 Tsyganova A.V., Tsyganov V.E., Borisov A.U., Tikhonovich I.A., Brewin N.

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