ANALYSIS OF INTERACTION OF PEA (Pisum sativum L.) SYMBIOTIC GENES Sym33 AND Sym42 WHOSE MUTATIONS RESULT IN ABNORMALITES DURING INFECTION THREAD DEVELOPMENT

Abstract


Using pea single mutant lines SGEFix–-2 (sym33) and RisFixV (sym42), which are characterized by different abnormalities during symbiotic nodule development, including thickening of infection threads’ walls, a double mutant RBT4 line, carrying a pair of symbiotic genes sym33 and sym42 was constructed. The epistasis of the mutant allele sym33 over the mutant allele sym42 with respect to the histological and ultrastructural organisation of nodules was shown. Thus, it was demonstrated that Sym33 gene functions earlier in symbiotic nodule development than Sym42 gene.

Viktor E Tsyganov

All-Russia Research Institute for Agricultural Microbiology, Saint-Petersburg, RF

Email: viktor_tsyganov@arriam.spb.ru

Anna V Tsyganova

All-Russia Research Institute for Agricultural Microbiology, Saint-Petersburg, RF

Email: anna_khodorenko@arriam.spb.ru

Vera A Voroshilova

Contract Drug Development Company “PSI”, St. Petersburg, RF

Email: vera.voroshilova@psi-cro.com

Aleksey U Borisov

All-Russia Research Institute for Agricultural Microbiology, Saint-Petersburg, RF

Email: ayborisov@yandex.ru

Igor A Tikhonovich

All-Russia Research Institute for Agricultural Microbiology, Saint-Petersburg, RF

Email: . contact@arriam.spb.ru

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Copyright (c) 2012 Tsyganov V.E., Tsyganova A.V., Voroshilova V.A., Borisov A.U., Tikhonovich I.A.

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