Metabolomics of senescence of heterotrophic suspension cultures of Nicotiana tabacum L. VBI-0



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Abstract

Background. Heterotrophic cell cultures are widely used as a model in plant biology. During a culture cycle the composition of the medium changes, the sucrose is depleted, and the density increases. Finally, arrest of a growth is followed by cell death in a short time. These processes are accompanied with physiological alterations, corresponding to senescence.

Materials and methods. Nicotiana tabacum VBI-0 cells were cultured in suspension MS medium supplied with 3% sucrose. Cells were sampled at 7th day, during intensive growth, and at 28th day, when the culture was in the stationary phase. The GC-MS method was used to profile the metabolites.

Results. Sucrose depletion in media caused starvation of heterotrophic tobacco cell culture and was associated with a decrease in the accumulation of free amino acids. At the same time, the level of pentoses and complex sugars, including sucrose, increased. But at the same time, the levels of glucose and fructose were not changed significantly and levels of hexose phosphates decreased. Also, cells during culture senescence showed higher levels of accumulation of malate, pyruvate and some other carboxylates.

Conclusion. The metabolomic data indicate that culture senescence was associated with a drop in the level of biosynthesis, a decrease in the activity of the upper part of glycolysis, and the accumulation of complex sugars, pentoses and carboxylates.

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About the authors

Roman K. Puzanskiy

Komarov Botanical Institute of the Russian Academy of Sciences; Saint Petersburg State University

Email: puzansky@yandex.ru
ORCID iD: 0000-0002-5862-2676
SPIN-code: 6399-2016

Cand. Sci. (Biol.), Research Associate, Laboratory of Analytical Phytochemistry; Department of Plant Physiology and Biochemistry

Russian Federation, Saint Petersburg; Saint Petersburg

Anastasija A. Kirpichnikova

Saint Petersburg State University

Email: nastin1972@mail.ru
ORCID iD: 0000-0001-5133-5175
SPIN-code: 9960-9527

Junior Researcher, Department of Plant Physiology and Biochemistry, Faculty of Biology

Russian Federation, 7/9, Universitetskaya embankment, Saint-Petersburg, 199034

Alexey L. Shavarda

Komarov Botanical Institute of the Russian Academy of Sciences; Saint Petersburg State University

Email: stachyopsis@gmail.com
ORCID iD: 0000-0003-1778-2814
SPIN-code: 5637-5122

Cand. Sci. (Biol.), Head of Laboratory of Analytical Phytochemistry; Center for Molecular and Cell Technologies

Russian Federation, Saint Petersburg; Saint Petersburg

Vladislav V. Yemelyanov

Saint Petersburg State University

Email: bootika@mail.ru
ORCID iD: 0000-0003-2323-5235
SPIN-code: 9460-1278

Cand. Sci. (Biol.), Associate Professor, Department of Genetics and Biotechnology

Russian Federation, Saint Petersburg

Maria F. Shishova

St. Petersburg State University

Author for correspondence.
Email: mshishova@mail.ru
ORCID iD: 0000-0003-3657-2986
SPIN-code: 7842-7611
https://bio.spbu.ru/staff/id200_mfsh.php

Dr. Sci., Professor, Department of Plant Physiology, Faculty of Biology

Russian Federation

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