The effect of arbuscular mycorrhiza on gene expression of sweet family in Medicago lupulina under conditions of high available phosphorus level
- Authors: Kudriashova T.R.1,2, Kryukov A.A.1, Gorbunova A.O.1, Gorenkova A.I.1,3, Kovalchuk A.I.1, Shishova M.F.3, Yurkov A.P.1
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Affiliations:
- All-Russia Research Institute for Agricultural Microbiology
- Peter the Great St. Petersburg Polytechnic University
- Saint Petersburg State University
- Issue: Vol 23, No 1 (2025)
- Pages: 19-32
- Section: Genetic basis of ecosystems evolution
- Submitted: 31.08.2024
- Accepted: 04.12.2024
- Published: 19.04.2025
- URL: https://journals.eco-vector.com/ecolgenet/article/view/635482
- DOI: https://doi.org/10.17816/ecogen635482
- ID: 635482
Cite item
Abstract
BACKGROUND: According to modern concepts, the SWEET family may be the only family of plant sugar transporters that includes genes specifically expressed during the formation and development of plant symbiosis with fungi of arbuscular mycorrhiza. The data on the key genetic markers of the development of effective arbuscular mycorrhiza symbiosis can contribute an active development of organic agriculture in various conditions of phosphorus availability in the soil.
AIM: to evaluate the effect of arbuscular mycorrhiza on the expression of SWEET genes in M. lupulina L. during key stages of host plant development (stages of leaves rosette, stooling initiation, stooling, lateral branching initiation, lateral branching and flowering).
MATERIALS AND METHODS: The study was performed using a highly efficient plant-microbial system “Medicago lupulina + Rhizophagus irregularis” grown under conditions with a high content of available phosphorus in the substrate.
RESULTS: Under condition of high phosphorus level in the substrate it was shown for the first time the MlSWEET1b and MlSWEET3c genes in M. lupulina leaves were characterized by specific expression during mycorrhization.
CONCLUSIONS: MlSWEET1b and MlSWEET3c and their orthologs can be considered as marker genes of effective symbiosis development, as a tool of biotechnology to increase agricultural productivity with using biostimulants based on arbuscular mycorrhiza fungi.
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About the authors
Tatyana R. Kudriashova
All-Russia Research Institute for Agricultural Microbiology; Peter the Great St. Petersburg Polytechnic University
Email: t.kudryashova@arriam.ru
ORCID iD: 0000-0001-5120-7229
SPIN-code: 6716-9431
Russian Federation, Pushkin, Saint Petersburg; Saint Petersburg
Alexey A. Kryukov
All-Russia Research Institute for Agricultural Microbiology
Email: aa.krukov@arriam.ru
ORCID iD: 0000-0002-8715-6723
SPIN-code: 4685-2723
Cand. Sci. (Biology)
Russian Federation, Pushkin, Saint PetersburgAnastasia O. Gorbunova
All-Russia Research Institute for Agricultural Microbiology
Email: a.gorbunova@arriam.ru
SPIN-code: 3515-6450
Russian Federation, Pushkin, Saint Petersburg
Anastasia I. Gorenkova
All-Russia Research Institute for Agricultural Microbiology; Saint Petersburg State University
Email: nastya.gorenkova.2016@mail.ru
SPIN-code: 3888-9050
Russian Federation, Pushkin, Saint Petersburg; Saint Petersburg
Anastasia Igorevna Kovalchuk
All-Russia Research Institute for Agricultural Microbiology
Email: a.kovalchuk@arriam.ru
SPIN-code: 7051-0285
Russian Federation, Pushkin, Saint Petersburg
Maria F. Shishova
Saint Petersburg State University
Email: mshishova@mail.ru
ORCID iD: 0000-0003-3657-2986
SPIN-code: 7842-7611
Dr. Sci. (Biology), Professor
Russian Federation, Saint PetersburgAndrey P. Yurkov
All-Russia Research Institute for Agricultural Microbiology
Author for correspondence.
Email: ap.yurkov@arriam.ru
ORCID iD: 0000-0002-1072-5166
SPIN-code: 9909-4280
Cand. Sci. (Biology)
Russian Federation, Pushkin, Saint PetersburgReferences
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