Association between cytokine gene polymorphisms and squamous cell lung cancer depending on the duration of smoking in men

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Abstract


Background. Squamous cell lung cancer (SCLC) is the most common form of lung cancer among men smokers. Mediators and products of inflammation can contribute to the initiation of carcinogenesis in smokers. The aim of study is to investigate the association between IL1B(rs1143634, rs16944), IL1RN (rs2234663), TNFA (rs1800629), IL6 (rs1800795), IL10 (rs1800896) genes and SCLC depending on the duration of smoking in men.

Materials and methods. We studied 324 patients with SCLC and 168 healthy men smokers. The typing of the IL1RN(rs2234663) gene polymorphism has analyzed by polymerase chain reaction (PCR). Detection of the IL1B (rs1143634) and IL6 (rs1800795) polymorphisms have performed by restriction fragment length analysis, as a restriction enzyme TaqI was used. The IL1B (rs16944), TNFA(rs1800629) and IL10 (rs1800896) genes polymorphisms have determined through TaqMan-real-time PCR.

Results. The study showed, the age, duration of smoking and the number of cigarettes smoked per day were the main risk factors for SCLC in men (p < 0.0001). The IL6 -174G allele was detected an additional risk factor of SCLC at a smoking duration of less than 35 years (OR = 1.68; 95 % CI: 1.12-2.51; pcor = 0,04). No association of IL1B (rs1143634, rs16944), IL1RN (rs2234663), TNFA (rs1800629), and IL10 (rs1800896) genes with the risk of SCLC in men was identified.

Conclusion. The duration of smoking and rs1800795 IL6 gene polymorphism may influence on the susceptibility to SCLC in men. Our results can be useful in understanding the molecular mechanisms of development of SCLC.


Lyudmila A. Gordeeva

Author for correspondence.
gorsib@rambler.ru
FRC CCC SB RAS (Institute of Human Ecology SB RAS)
Russian Federation, Kemerovo

PhD, Leading Rof the Laboratory Immunogenetics

Stella A. Mun

stellamun@yandex.ru
FRC CCC SB RAS (Institute of Human Ecology SB RAS) Kemerovo

PhD, Senior Researcher of the Laboratory Immunogenetics

Elena N. Voronina

voronina_l@mail.ru
Institute of Chemical Biology and Fundamental Medicine SB RAS
Russian Federation, Novosibirsk

PhD, Researcher of the Laboratory Pharmacogenomics

Elena G. Polenok

ihe@list.ru
FRC CCC SB RAS (Institute of Human Ecology SB RAS)
Russian Federation, Kemerovo

PhD, Leading Researcher of the Laboratory Immunochemistry

Alina D. Magatina

ihe@kemtel.ru
Regional Clinical Hospital
Russian Federation, Kemerovo

Biologist of the Genetic Consultation

Viktor A. Titov

05-guz-okod@kuzdrav.ru
Regional Clinical Oncology Dispensary
Russian Federation, Kemerovo

Chief of Thoracic Department

Svetlana E. Ragozhina

ragozhina@mail.ru
Regional Center of Blood
Russian Federation, Kemerovo

Assistant of Main Physician on Medicine

Ilgiz A. Vafin

stpk@kuzdrav.ru
Regional Center of Blood
Russian Federation, Kemerovo

Main Physician

Elizaveta L. Romanova

nev11@yandex.ru
Kemerovo State University (KemSU)
Russian Federation, Kemerovo

Learning of The Center for Continuing Education

Andrey N. Glushkov

ihe@list.ru
FRC CCC SB RAS (Institute of Human Ecology SB RAS)
Russian Federation, Kemerovo

Doctor of Medical Sciences, Deputy Director

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Supplementary files

Supplementary Files Action
1. Fig. 1. ROC-curve for the parameter duration of smoking and the test levels of sensitivity and specificity View (102KB) Indexing metadata

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Copyright (c) 2018 Gordeeva L.A., Mun S.A., Voronina E.N., Polenok E.G., Magatina A.D., Titov V.A., Ragozhina S.E., Vafin I.A., Romanova E.L., Glushkov A.N.

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