ПЕРСПЕКТИВЫ ИСПОЛЬЗОВАНИЯ МИКРОЯДЕРНОГО ТЕСТА НА ЛИМФОЦИТАХ КРОВИ ЧЕЛОВЕКА, КУЛЬТИВИРУЕМЫХ В УСЛОВИЯХ ЦИТОКИНЕТИЧЕСКОГО БЛОКА. ЧАСТЬ 1. ПРОЛИФЕРАЦИЯ КЛЕТОК



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Культивирование клеток периферической крови в условиях цитокинетического блока создает уникальную возможность проанализировать весь спектр изменений, характеризующих нестабильность генома. Задачей настоящей публикации является анализ современного состояния, возможностей и перспектив использования микроядерного теста на культуре лимфоцитов периферической крови человека. особое внимание уделяется тем направлениям исследований, которые относительно редко представлены в литературе - закономерностям пролиферации клеток, существованию физиологической обусловленности и генетической предрасположенности к развитию эффектов нестабильности генома; эффектам эмоционального стресса и адаптивному ответу к действию ионизирующей радиации. Материал представлен в 2-х последовательных публикациях. Его завершает обсуждение методических особенностей постановки культуры, результатов микроскопического анализа и идеологии получения конечного результата исследования.

Об авторах

Фаина Исааковна Ингель

Научно-исследовательский институт экологии человека и гигиены окружающей среды им. А.Н. Сысина, Москва, РФ

Email: fainaingel@mail.ru

Список литературы

  1. Гомес Л.А., Ярцев М.Н., Хахалин Л.Н. и др. Клинико-иммунологическая характеристика некоторых иммунодефицитных синдромов с ведущей Т-клеточной недостаточностью//Вопр. охр. мат. и дет. -1989. -№ 2. -С. 13-16.
  2. Гуськов А.С., Ингель Ф.И., Малышева А.Г. и др. Аэроионификация и ее влияние на состояние здоровья работающих//Гигиена и санитария. -2005. -№ 4. -С. 32-34.
  3. Ингель Ф.И., Юрченко В.В., Гуськов А.С. и др. Показатели пролиферативной активности и их связь с генетическими повреждениями лимфоцитов крови при культивировании в условиях цитокинетического блока//Вестник РАМН. -2006. -№ 4. -С. 41-46.
  4. Хусаинова Ш.Н., Ингель Ф.И., Петрова И.В. и др. Оценка состояния здоровья детей Приаралья//Гигиена и санитария. -2004. -№ 6. -С. 35-38.
  5. Abou-Eisha A., Marcos R., Creus A. Genotoxicity studies on the antimicrobial drug sulfamethoxazole in cultured human lymphocytes//Mutat. Res. -2004.-Vol. 564, N 1. -P.51-66.
  6. Andreassi M.G., Picano E., Del Ry S., et al. Chronic longterm nitrate therapy: possible cytogenetic effect in humans?//Mutagenesis -2001. -Vol. 16, N 6. -P.517-521.
  7. Bonder E.M., Mooseker M.S., Cytochalasin B. slows but does not prevent monomer addition at the barbed end of the actin filament//J. Cell Biol. -1986. -Vol.102, N 1. -P.282-288.
  8. Boreham D.R., Dolling J.A., Maves S.R., Siwarungsun N., Mitchel R.E. Dose-rate effects for apoptosis and micronucleus formation in gamma-irradiated human lymphocytes//Radiat. Res. -2000. -Vol.153, N 5 (Pt. 1). -P. 579-586.
  9. Bozsakyova E., Chalupa I., Sebova L., Slamenova D. Chromosomal aberrations, sister chromatid exchanges and micronuclei induced by pentoxifylline in in vitro cultivated Chinese hamster cells (V79) and human blood lymphocytes//Mutat. Res. -2001. -Vol. 18; 497(1-2). -P. 63-70.
  10. Brown S.S., Spudich J.A. Mechanism of action of cytochalasin: evidence that it binds to actin filament ends//J. Cell Biol. -1981. -Vol.88, N 3. -Р.487-491.
  11. Bukvic N., Gentile M., Susca F., et al. Sex chromosome loss, micronuclei, sister chromatid exchange and aging: a study including 16 centenarians.//Mutat. Res. -2001. -Vol. 498, N 12. -Р. 159-167.
  12. Chang W.P., Tsai M.S., Hwang J.S., et al. Follow-up in the micronucleus frequencies and its subsets in human population with chronic low-dose gamma-irradiation exposure//Mutat. Res. -1999. -Vol.428, N 1-2. -Р.99-105.
  13. Cloos J., Reid C.B., van der Sterre M.L. et al. A comparison of bleomycin induced damage in lymphocytes and primary oral fibroblasts and keratinocytes in 30 subjects.//Mutagenesis. -1999. -Vol.14, N 1. -Р. 87-93.
  14. Elsendoorn T.J., Weijl N.I., Mithoe S., et al. Chemotherapyinduced chromosomal damage in peripheral blood lymphocytes of cancer patients supplemented with antioxidants or placebo//Mutat. Res. -2001. -Vol. 498, N 1-2. -Р. 145-158.
  15. Erdinger L., Eckl P., Ingel F., et al. The Aral Sea disasterhuman biomonitoring of Hg, As, HCB,DDE, and PCBs in children living in Aralsk and Akchi, Kazakhstan//International Journal of Hygiene and Environmental Health. -2004. -Vol. 207. -P. 541-547.
  16. Fenech M., Bonassi S., Turner J., et al. Human Micro Nucleus project. Intra-and inter-laboratory variation in the scoring of micronuclei and nucleoplasmic bridges in binucleated human lymphocytes. Results of an international slide-scoring exercise by the HUMN project//Mutat. Res. -2003. -Vol. 534, N 1-2. -Р. 45-64.
  17. Fenech M., Morley A. Solutions to the kinetic problem in the micronucleus assay//Cytobios. -1985. -Vol. 43, N 172-173. -Р. 233-246.
  18. Fenech M. A mathematical model of the in vitro micronucleus assay predicts false negative results if micronuclei are not specifically scored in binucleated cells or in cells that have completed one nuclear division//Mutagenesis. -2000. -N 4. -Р. 329-336.
  19. Feuerstein N., Sahai A., Anderson W.B., et al. Differential phosphorylation events associated with phorbol ester effects on acceleration versus inhibition of cell growth.//Cancer Res. -1984. -Vol. 44, N 11. -Р. 5227-33.
  20. Ganguly B.B. Age-related alterations in cell division and cell cycle kinetics in control and trimethyltin-treated lymphocytes of human individuals//Biometals. -1995. -Vol. 8, N 3. -Р. 263-269.
  21. Garaj-Vrhovac V., Zeljezic D. Cytogenetic monitoring of croatian population occupationally exposed to a complex mixture of pesticides//Toxicology. -2001. -Vol. 165, N 2-3. -Р. 153-162.
  22. Gonzalez Borroto J.I., Creus A., Marcos R. Genotoxic evaluation of the furylethylene derivative 2-furyl-1-nitroethene in cultured human lymphocytes//Mutat. Res. -2001. -Vol. 497, N 1-2. -Р. 177-184.
  23. He J.L., Jin H.Y., Jin L.F., Gao S.Y. Monitoring of human exposure to radiation with the binucleated lymphocyte micronucleus assay//Biomed. Environ Sci. -2000. -Vol. 13, N 11. -Р. 32-66.
  24. Heimers A. Cytogenetic analysis in human lymphocytes after exposure to simulated cosmic radiation which reflects the inflight radiation environment//Int. J. Radiat. Biol. -1999. -Vol. 75, N 6. -Р. 691-698.
  25. Huber R., Braselmann H., Bauchinger M. Screening for interindividual differences in radiosensitivity by means of the micronucleus assay in human lymphocytes//Radiat. Environ Biophys. -1989. -Vol. 28, N 2. -Р. 113-120.
  26. Joksic G., Petrovic S. Lack of adaptive response of human lymphocytes exposed in vivo to low doses of ionizing radiation//J. Environ Pathol. Toxicol. Oncol. -2004. -Vol. 23, N 3. -Р. 195-206.
  27. Kirch-Volders M., Fenech M. Inclusion of micronuclei in nondivided mononuclear lymphocytes and necrosis/apoptosis may provide a more comprehensive cytokinesis block micronucleus assay for biomonitoring purposes//Mutagenesis. -2001. -Vol. 16, N 1. -Р.51-58.
  28. Kocisova J., Sram R.J. The micronucleus test in human peripheral lymphocytes//Cesk Farm. -1990. -Vol. 39, N 3. -Р. 131-133.
  29. Kollmar O., Menger M.D., Schilling M.K. Macrophage inflammatory protein-2 contributes to liver resectioninduced acceleration of hepatic metastatic tumor growth//World J. Gastroenterol. -2006. -Vol. 12, N 6. -Р. 858-867.
  30. Kryscio A., Ulrich Muller W.U., Wojcik A., et al. A cytogenetic analysis of the long-term effect of uranium mining on peripheral lymphocytes using the micronucleus-centromere assay//Int. J. Radiat Biol. -2001. -Vol. 77, N 11. -Р. 1087-1093.
  31. Lee T.K., O'Brien K., Eaves G.S., et al. Effect of blood storage on radiation-induced micronuclei in human lymphocytes//Mutat. Res. -1999. -Vol. 444, N 1. -Р.201-206.
  32. MacLean-Fletcher S., Pollard T.D. Mechanism of action of cytochalasin B on actin//Cell. -1980. -Vol. 20, N 2. -Р. 329-341.
  33. Mante S.D., Flashner M., Tanenbaum S.W. Effects of cytochalasin A on the morphology of plasmodia and sclerotia of Physarum polycephalum//Cytobiologie. -1978. -Vol. 17, N 1. -Р. 10-22.
  34. Martelli A., Robbiano L., Cosso M., et al. Comparison of micronuclei frequencies in mono-, bi-and poly-nucleated lymphocytes from subjects of a residential subrup and subjects living near a metallurgical plant//Mutat. Res. -2000. -Vol. 470. -Р. 211-219.
  35. Mooseker M.S., Coleman T.R., Conzelman K.A. Calcium and the regulation of cytoskeletal assembly, structure and contractility//Ciba Found Symp. -1986. -Vol. 122. -Р. 232-249.
  36. Parry J.M. A proposal for a new OECD Guideline for the in vitro micronucleus test. (http://www.oecd.org/dataoecd).
  37. Pascoe S. A, Stemp G. A modified method and staining technique for the in vitro micronucleus test in human lymphocytes using cytochalasin B//Mutat. Res. -1990. -Vol. 234, N 3-4. -Р. 253-255.
  38. Pastor S., Gutierrez S., Creus A., Xamena N., Piperakis S., Marcos R. Cytogenetic analysis of Greek farmers using the micronucleus assay in peripheral lymphocytes and buccal cells//Mutagenesis. -2001. -Vol. 16, N 6. -Р. 539-545.
  39. Rosefort C, Fauth E, Zankl H. Micronuclei induced by aneugens and clastogens in mononucleate and binucleate cells using the cytokinesis block assay//Mutagenesis. -2004. -Vol. 19, N 4. -Р. 277-284.
  40. Schmid W. The micronucleus test for cytogenetic analysis/In: Chemical Mutagens. Principles and methods for their detection; (Ed. Hollaender A.) -N-Y-L., 1976. -Vol. 4. -Р. 31-53.
  41. Shimizu N., Iton N., Utiyama H., Wahl G.M. Selective entrapment of extrachromosomally amplified DNA by nuclear budding and micronucleation during S phase//J. Cell Biol. -1998. -Vol. 140, N 6, -Р. 1307-1320.
  42. Shimizu N., Hashizume T., Shingaki K., Kawamoto J. Amplification of plasmids containing a mammalian replication initiation region is mediated by controllable conflict between replication and transcription//Cancer Research. -2003. -Vol. 63. -P. 5281-5290.
  43. Shimizu N., Kamezaki F., Shigematsu Sh. Tracing of microinjected DND in live cells reveals the intracellular behavior and elimination of extrachromosomal genetic material//Nucleic Acid Research. -2005. -Vol. 33, N 19. -P. 6296-6307.
  44. Shimizu N, Shimura T., Tanaka T. Selective elimination of acentric double minutes from cancer cell through the extrusion of micronuclei//Mutation Research. -2000. -Vol. 448, N 1. -Р. 81-90.
  45. Slonina D., Klimek M., Szpytma T., Gasinska A. Comparison of the radiosensitivity of normal-tissue cells with normal-tissue reactions after radiotherapy//Int. J. Radiat Biol. -2000. -Vol. 76, N 9. -Р. 1255-1264.
  46. Small J.V. Microfilament-based motility in non-muscle cells//Curr Opin Cell Biol. -1989. -Vol. 1, N 1. -Р. 75-79.
  47. Stavropoulou V., Xie J., Henriksson M., et al. Mitotic Infidelity and Centrosome Duplication Errors in Cells Overexpressing Tripeptidyl-Peptidase II//Cancer Research. -2005. -Vol. 65. -Р. 1361-1368.
  48. Swain D.E., Mill A.J. An 11 year follow-up of individual radiation responses as assessed by micronuclei induction in peripheral blood lymphocytes//J. Radiol. Prot. -2000. -Vol. 20, N 2. -Р. 189-196.
  49. Tanaka K., Tchaijunusova N.J., Takatsuji T., et al. High incidence of micronuclei in lymphocytes from residents of the area near the Semipalatinsk nuclear explosion test site//J. Radiat Res (Tokyo). -2000. -Vol. 41, N 1. -Р. 45-54.
  50. Thierens H., Vral A., de Ridder L., Touil N., Kirsch-Volders M., Lambert V., Laurent C. Inter-laboratory comparison of cytogenetic endpoints for the biomonitoring of radiological workers//Int. J. Radiat. Biol. -1999. -Vol. 75, N 1. -Р.34.
  51. Titenko-Holland N., Jacob R.A., Shang N. et al. Micronuclei in lymphocytes and exfoliated buccal cells of postmenopausal women with dietary changes in filate//Мat. Res. -1998. -Vol. 417. -Р. 101-114.
  52. Touil N., Elhajouji A., Thierens H., Kirsch-Volders M. Analysis of chromosome loss and chromosome segregation in cytokinesis-blocked human lymphocytes: non-disjunction is the prevalent mistake in chromosome segregation produced by low dose exposure to ionizing radiation//Mutagenesis. -2000. -Vol.15, N 1. -Р. 1-7.
  53. Vaglenov A., Nosko M., Georgieva R., et al. Genotoxicity and radioresistance in electroplating workers exposed to chromium//Mutat. Res. -1999. -Vol. 446, N 1. -Р. 23-34.
  54. Venkatachalam P., Solomon F.D., Prabhu B.K., et al. Estimation of dose in cancer patients treated with fractionated radiotherapy using translocation, dicentrics and micronuclei frequency in peripheral blood lymphocytes//Mutat. Res. -1999. -Vol. 429, N 1. -Р. 1-12.
  55. Yager J.W., Sorsa M., Selvin S. Micronuclei in cytokinesisblocked lymphocytes as an index of occupational exposure to alkylating cytostatic drugs//IARC Sci. Publ. -1988. -Vol. 89. -Р. 213-216.
  56. Zeljezic D., Garaj-Vrhovac V. Chromosomal aberrations, micronuclei and nuclear buds induced in human lymphocytes by 2,4-dichlorophenoxyacetic acid pesticide formulation//Toxicology. -2004. -Vol. 200. -Р. 39-47.
  57. Zenzen V., Fauth E., Zankl H., et al. Mutagenic and cytotoxic effectiveness of zinc dimethyl and zinc diisononyldithiocarbam ate in human lymphocyte cultures//Mutat. Res. -2001. -Vol. 497, N 1-2. -Р. 89-99.

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