Researching the mechanisms of gene РНО3 regulation de pending on nitrogen source in medium in yeast Sacharomyces cere visiae



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Abstract

Delicate regulation of gene expression is performed through transcriptional factors competition for promoters that provides punctual cell response to environmental changes. Gene PHO3 of yeast S. cerevisiae encodes the constitutive acid phosphatase. In this work we researched genetic control of the gene PHO3 expression in response to nitrogen source in medium. PHO3 expression level was proved to decrease while yeast using poor nitrogen source like urea. Possible regulatory mechanisms for gene PHO3 were revealed involving repressor of nitrogen regulated genes Gzf3p and ubiquitin ligase Rsp5p

About the authors

Vladimir A Savinov

Saint-Petersburg State University., Saint-Petersburg, RF

Email: vova.savinoff@gmail.com 7-9, Universitetskaya nab., St.Petersburg, 199034, Russia

Anastasia I Fizikova

Saint-Petersburg State University., Saint-Petersburg, RF

Email: vova.savinoff@gmail.com 7-9, Universitetskaya nab., St.Petersburg, 199034, Russia

Andrey M Rumyantsev

Saint-Petersburg State University., Saint-Petersburg, RF

Email: rumyantsev-am@mail.ru

Elena V Sambuk

Saint-Petersburg State University, Saint-Petersburg, RF

Email: esambuk@mail.ru

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Copyright (c) 2011 Savinov V.A., Fizikova A.I., Rumyantsev A.M., Sambuk E.V.

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