Correction of vaginal dysbiosis with cavitated solution of chlorhexidine in the 1st trimester of pregnancy: effectiveness and safety

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Abstract

Aim. The aim of this study was to evaluate changes of vaginal microbiocenosis composition of pregnant women in first trimester after irrigation of the vagina with cavitated 0.05% solution of chlorhexidine and to find out if the following dotation of Lactobacillus spp. was needed. Materials and methods. 104 women planning induced termination of pregnancy in first trimester by vacuum aspiration were examined in order to estimate efficiency of vaginal microbiocenosis correction using irrigation of the vagina with cavitated 0.05% solution of chlorhexidine and to find out if the following dotation of Lactobacillus spp. was needed. All women had undergown irrigation of vagina and exocervix with 0.05% solution of chlorhexidine in order to prepare them for surgical abortion. Vaginal microbiocenosis before and after this procedure was evaluated by microscopy and real-time PCR (test Femoflor-16, DNA-Technology LLC, Moscow). Results. It was established that in patients with normocenosis the amount of Lactobacillus spp. decreased accordingly to the decrease of general bacterial load in the vagina at that the proportion of Lactobacillus spp. in microbiota stayed almost the same and was more than 90% in all examined women. In women with dysbiosis the decrease of general bacterial load in the vagina after irrigation with cavitated 0.05% solution of chlorhexidine happens mostly due to absolute and relative content of opportunistic microflora in microbiome. However initially low level of Lactobacillus does not allow reaching their optimal level after the first treatment stage. In these cases, the necessity of dotation of Lactobacillus spp. on the second stage of treatment is practically assured. Conclusions. The data demonstrated safety of use cavitated 0.05% solution of chlorhexidine for irrigation of the vagina in first trimester of pregnancy, that allow apply the method for prevention of infected complications before invasive procedure independently of primary composition of vaginal microbiota. Also this method demonstrated high effectiveness as approach for treatment of vaginal dysbiosis. However the continuing deficiency of lactobacilli requires their dotation on following stage.

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About the authors

Ekaterina Sergeevna Voroshilina

GBOU VPO USMU MZ RF

Email: voroshilina@gmail.com
doctor of medical science, associated professor, Department of Microbiology, Virology and Immunilogy

Danila Leonidovich Zornikov

GBOU VPO USMU MZ RF

PhD-student, Department of Microbiology, Virology and Immunilogy

Evgeniy Eduardovich Plotko

GBOU VPO USMU MZ RF

Associated professor, department of Obstetrics and Gynaecology

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Copyright (c) 2014 Voroshilina E.S., Zornikov D.L., Plotko E.E.

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СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
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