An improved method for experimental modeling of premature ovarian insufficiency in Wistar rats
- 作者: Zakurayeva K.A.1, Yarmolinskaya M.I.1, Adamenkov N.A.2,3, Potapova E.V.3
-
隶属关系:
- The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
- Oryol Regional Clinical Hospital
- Turgenev Oryol State University
- 期: 卷 72, 编号 6 (2023)
- 页面: 55-62
- 栏目: Original Research
- ##submission.dateSubmitted##: 12.09.2023
- ##submission.dateAccepted##: 03.11.2023
- ##submission.datePublished##: 15.12.2023
- URL: https://journals.eco-vector.com/jowd/article/view/569228
- DOI: https://doi.org/10.17816/JOWD569228
- ID: 569228
如何引用文章
详细
BACKGROUND: Despite an enormous progress in the field of reproductive endocrinology, the mechanisms of premature ovarian insufficiency have not been fully understood so far. Knowledge of the supposed causes and mechanisms underlying the development of premature ovarian insufficiency has expanded significantly in recent years, mainly due to advances in genetics and the creation of models for experimental research. The experimental modeling of premature ovarian insufficiency, which is as close as possible to the origin and development of the corresponding disease in humans, is effectively used to elaborate promising therapeutic approaches, in particular, to test new drugs or biologically active agents in order to study their preventive or curative effects. This article discusses the main methods of the experimental modeling of POI in laboratory animals. We have herein summarized the literature data related to the development of the clinical and morphological picture of the disease, with the advantages and disadvantages of each of the models analyzed.
AIM: The aim of this study was to modify the premature ovarian insufficiency modeling methodology to substantiate the effectiveness and to test new directions in drug therapy with subsequent implementation in clinical practice.
MATERIALS AND METHODS: The new method is based on the creation of an experimental premature ovarian insufficiency model in female Wistar rats. To obtain this model, experimental rats were randomized into two groups: 1) animals receiving cyclophosphamide at a dose of 150 mg/kg b.w. subcutaneously once (n = 10); 2) animals receiving 2 ml of saline intraperitoneally once (n = 9). On day10, the animals were removed from the experiment. After autopsy, a visual assessment of morphological changes in the ovaries was carried out, and a right-sided ovariectomy was performed.
RESULTS: When using the proposed model, the reproducibility rate of POI has reached 100%.
CONCLUSIONS: Our modification of the method ensures the creation of a highly reproducible premature ovarian insufficiency model in Wistar rats. Another significant advantage of this modeling method is its ease of implementation and economic efficiency.
全文:
作者简介
Karina Zakurayeva
The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
编辑信件的主要联系方式.
Email: zakuraevak@icloud.com
ORCID iD: 0000-0002-8128-306X
SPIN 代码: 5215-7869
俄罗斯联邦, Saint Petersburg
Maria Yarmolinskaya
The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
Email: m.yarmolinskaya@gmail.com
ORCID iD: 0000-0002-6551-4147
SPIN 代码: 3686-3605
MD, Dr. Sci. (Med.), Professor of the Russian Academy of Sciences
俄罗斯联邦, Saint PetersburgNikita Adamenkov
Oryol Regional Clinical Hospital; Turgenev Oryol State University
Email: nikita-ad@mail.ru
ORCID iD: 0000-0002-0238-2941
SPIN 代码: 3348-8250
俄罗斯联邦, Oryol; Oryol
Elena Potapova
Turgenev Oryol State University
Email: potapova_ev_ogu@mail.ru
ORCID iD: 0000-0002-9227-6308
SPIN 代码: 9315-8770
Cand. Sci. (Tech.), Assistant Professor
俄罗斯联邦, Oryol参考
- Kapoor E. Premature ovarian insufficiency. Curr Opin Endocr Metab Res. 2023;28. doi: 10.1016/j.coemr.2023.100435
- Fernando WD, Vincent A, Magraith K. Premature ovarian insufficiency and infertility. Aust J Gen Pract. 2023;52(1-2):32–38. doi: 10.31128/AJGP-08-22-6531
- Wesevich V, Kellen AN, Pal L. Recent advances in understanding primary ovarian insufficiency. F1000Res. 2020;9. doi: 10.12688/F1000RESEARCH.26423.1
- Chen M, Jiang H, Zhang C. Selected genetic factors associated with primary ovarian insufficiency. Int J Mol Sci. 2023;24(5):4423. doi: 10.3390/IJMS24054423
- Coulam CB, Adamson SC, Annegers JF. Incidence of premature ovarian failure. Obstetrics and Gynecology. 1986;67(4):604–606. doi: 10.1097/00006254-198742030-00020
- Luborsky JL, Meyer P, Sowers MF, et al. Premature menopause in a multi-ethnic population study of the menopause transition. Hum Reprod. 2003;18(1):199–206. doi: 10.1093/HUMREP/DEG005
- Lagergren K, Hammar M, Nedstrand E, et al. The prevalence of primary ovarian insufficiency in Sweden; a national register study. BMC Womens Health. 2018;18(1):175. doi: 10.1186/s12905-018-0665-2
- Golezar S, Ramezani Tehrani F, Khazaei S, et al. The global prevalence of primary ovarian insufficiency and early menopause: a meta-analysis. Climacteric. 2019;22(4):403–411. doi: 10.1080/13697137.2019.1574738
- Li K, Chen G, Hou H, et al. Analysis of sex hormones and menstruation in COVID-19 women of child-bearing age. Reprod Biomed Online. 2021;42(1):260–267. doi: 10.1016/J.RBMO.2020.09.020
- Ding T, Wang T, Zhang J, et al. Analysis of ovarian injury associated with COVID-19 disease in reproductive-aged women in Wuhan, China: an observational study. Front Med. 2021;8. doi: 10.3389/FMED.2021.635255
- Henarejos-Castillo I, Sebastian-Leon P, Devesa-Peiro A, et al. SARS-CoV-2 infection risk assessment in the endometrium: viral infection-related gene expression across the menstrual cycle. Fertil Steril. 2020;114(2):223–232. doi: 10.1016/j.fertnstert.2020.06.026
- Herr D, Bekes I, Wulff C. Local renin-angiotensin system in the reproductive system. Front Endocrinol. 2013;4:150. doi: 10.3389/fendo.2013.00150
- Webber L, Davies M, Anderson R, et al. ESHRE Guideline: management of women with premature ovarian insufficiency. Hum Reprod. 2016;31(5):926–937. doi: 10.1093/HUMREP/DEW027
- Tugrul Ersak D, Yilmaz N, Cavkaytar S, et al. Investigation of the role of serum telomerase levels in patients with occult primary ovarian insufficiency: a prospective cross-sectional study. J Obstet Gynaecol. 2022;42(3):485–489. doi: 10.1080/01443615.2021.1916807
- Mohamed SA, Shalaby S, Brakta S, et al. Umbilical cord blood mesenchymal stem cells as an infertility treatment for chemotherapy induced premature ovarian insufficiency. Biomedicines. 2019;7(1):7. doi: 10.3390/biomedicines7010007
- Kalich-Philosoph L, Roness H, Carmely A, et al. Cyclophosphamide triggers follicle activation and “burnout”; AS101 prevents follicle loss and preserves fertility. Sci Transl Med. 2013;5(185). doi: 10.1126/scitranslmed.3005402
- Li H, Song D, Zhong Y, et al. Human umbilical cord mesenchymal stem cells therapy in cyclophosphamide-induced premature ovarian failure rat model. Biomed Res Int. 2016;2016. doi: 10.1155/2016/2517514
- Manshadi MD, Navid S, Hoshino Y, et al. The effects of human menstrual blood stem cells-derived granulosa cells on ovarian follicle formation in a rat model of premature ovarian failure. Microsc Res Tech. 2019;82(6):635–642. doi: 10.1002/JEMT.23120
- Liu T, Li Q, Wang S, et al. Transplantation of ovarian granulosa-like cells derived from human induced pluripotent stem cells for the treatment of murine premature ovarian failure. Mol Med Rep. 2016;13(6):5053–5058. doi: 10.3892/MMR.2016.5191
- Tan R, He Y, Zhang S, et al. Effect of transcutaneous electrical acupoint stimulation on protecting against radiotherapy-induced ovarian damage in mice. J Ovarian Res. 2019;12(1):65. doi: 10.1186/S13048-019-0541-1
- Rajkovic A, Pangas SA, Ballow D, et al. NOBOX deficiency disrupts early folliculogenesis and oocyte-specific gene expression. Science. 2004;305(5687):1157–1159. doi: 10.1126/SCIENCE.1099755
- Zhou Y, Qin Y, Qin Y, et al. Wdr62 is involved in female meiotic initiation via activating JNK signaling and associated with POI in humans. PLoS Genet. 2018;14(8). doi: 10.1371/JOURNAL.PGEN.1007463
- Li N, Liu L. Mechanism of resveratrol in improving ovarian function in a rat model of premature ovarian insufficiency. J Obstet Gynaecol Res. 2018;44(8):1431–1438. doi: 10.1111/JOG.13680