Pathomorphology of gravid endometrium in patients with recurrent miscarriage in early periods with underlying chronic endometritis and the presence of low-functional alleles in folate cycle genes

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Abstract

AIM: This study aimed to identify the peculiarities of pathomorphological alterations in the gravid endometrium and polymorphism of the folate cycle genes in female patients with recurrent early miscarriage and underlying chronic endometritis.

MATERIALS AND METHODS: The uterine mucosa of women who had uncomplicated pregnancies and decided to have an artifactual abortion within 5–12 weeks (control group, n = 36) and patients with recurrent miscarriage with underlying chronic endometritis (main group, n = 83) was histologically examined. The polymorphism of the folate cycle genes MTHFR С677Т (rs 1801133), MTHFR A1298C (rs 1801131), and MTRR A66G (rs 1801394) was studied in the female patients of the main group and women with the preserved reproductive function (comparison group, n = 52) through real-time polymerase chain reaction (PCR).

RESULTS: Fibroblast-like cells (FLCs) of the stroma sequentially differentiated into predecidual cells from 5 weeks to 12 weeks of uncomplicated pregnancy. In the case of recurrent miscarriage against background chronic endometritis, defect was observed in the differentiation of FLCs of the endometrial stroma. This finding was associated with a decrease in the morphometric parameters of the cells and an increase in the frequency of the occurrence of the low-functional allele 677T in the methylenetetrahydrofolate reductase gene.

CONCLUSION: The course and outcome of pregnancy in the first trimester were negatively affected by the structural inadequacy of endometrial stromal cells in recurrent miscarriage and chronic endometritis combined with the presence of the MTHFR 677T allele in a woman’s genotype.

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About the authors

Lybov P. Peretyatko

V.N. Gorodkov Ivanovo Research Institute of Maternity and Childhood

Email: peretiatko2011@yandex.ru
ORCID iD: 0000-0003-1047-6312
SPIN-code: 2795-9185
ResearcherId: В-1176-2017

MD, Dr.Sci.(Med.), Professor, Honored Doctor of the Russian Federation, Сhief Researcher of the Pathomorphology and Electron Microscopy Laboratory

Russian Federation, Ivanovo

Irina N. Fetisova

V.N. Gorodkov Ivanovo Research Institute of Maternity and Childhood; Ivanovo State Medical Academy

Email: ivgenlab@gmail.com
ORCID iD: 0000-0002-5769-1645
SPIN-code: 5995-0391
ResearcherId: B-1288-2017

MD, Dr.Sci.(Med.), Associate Professor, Leading Researcher of the Clinical Biochemistry and Medical Genetics Laboratory

Russian Federation, Ivanovo; Ivanovo

Natalya V. Fateeva

V.N. Gorodkov Ivanovo Research Institute of Maternity and Childhood

Author for correspondence.
Email: nata6120@bk.ru
ORCID iD: 0000-0002-4077-3310
SPIN-code: 5817-1380
ResearcherId: А-9999-2017

Junior Researcher of the Pathomorphology and Electron Microscopy Laboratory

Russian Federation, Ivanovo

Roman A. Kuznetsov

V.N. Gorodkov Ivanovo Research Institute of Maternity and Childhood

Email: rakuzz@mail.ru
ORCID iD: 0000-0001-6061-2955
SPIN-code: 1285-4151
ResearcherId: B-1106-2017

MD, Cand.Sci.(Med.), Junior Researcher of the Pathomorphology and Electron Microscopy Laboratory

Russian Federation, Ivanovo

Nikolay S. Fetisov

V.N. Gorodkov Ivanovo Research Institute of Maternity and Childhood

Email: ivgenlab@gmail.com
ORCID iD: 0000-0002-6455-477X
SPIN-code: 5191-3751
ResearcherId: E-3831-2017

MD, Cand.Sci.(Med.), Junior Researcher of the Clinical Biochemistry and Medical Genetics Laboratory

Russian Federation, Ivanovo

References

  1. Tetruashvili NK. Recurrent abortion. Akusherstvo i Ginekologija: Novosti, Mnenija, Obuchenie. 2017;(4):70-87. (In Russ). doi: 10.24411/2303-9698-2017-00010
  2. Sidel’nikova VM, Sukhikh GT. Nevynashivaniye beremennosti: rukovodstvo dlya vrachey. Moscow: MIA; 2011. (In Russ).
  3. Karp GJA, Radzinskiy VE, editors. Privychnoye nevynashivaniye beremennosti: prichiny, versii i kontraversii, lecheniye. Moscow: GEOTAR-Media; 2017. (In Russ).
  4. Puente E, Alonso L, Laganà AS, et al. Chronic endometritis: old problem, novel insights and future challenges. International Journal of Fertility & Sterility. 2020;13(4):250-6. doi: 10.22074/ijfs.2020.5779
  5. Cicinelli E, Matteo M, Tinelli R, et al. Chronic endometritis due to common bacteria is prevalent in women with recurrent miscarriage as confirmed by improved pregnancy outcome after antibiotic treatment. Reproductive Sciences. 2014;21(5):640-7. doi: 10.1177/1933719113508817
  6. Peretyatko LP, Kuznetsov RA, Fateyeva NV. Patomorfologiya vorsinchatogo khoriona i detsidual’noy tkani endometriya pri zamershey beremennosti na fone khronicheskogo vospaleniya. In: Orekhova EYa, El’diyeva TM, Peretyatko LP. Nauchnoye otkrytiye: sbornik statey mezhdunarodnoy nauchnoy konferentsii; 28-29 January 2016; Karlovy Vary – Moscow. Kirov: Mezhdunarodnyy tsentr nauchno-issledovatel’skikh proyektov; 2016. P. 255-63. (In Russ).
  7. Radzinskiy VE. Folaty v XXI veke vne beremennosti. Tol’ko dokazannyye fakty. Moscow: Status Praesens; 2014. (In Russ).
  8. Fetisova IN, Malyshkina AI, Fetisov NS. Genetic polymorphism of folate cycle in women with incomplete pregnancy. Vestnik Ivanovskoj Medicinskoj Akademii. 2019;24(1):33-6. (In Russ).
  9. Kondrikov NI, Barinova IV. Patologiya matki: rukovodstvo dlya vrachey. 2nd ed. Moscow: Prakticheskaya meditsina; 2019. (In Russ).
  10. Pulikov AS, Peretyatko OV. Therapeutic pathomorphism of placenta decidual cells while ureaplasmosis during pregnancy. Fundamental Research. 2015;(1):337-41. (In Russ).
  11. Milovanov AP. Patologiya sistemy mat’ – platsenta – plod: rukovodstvo dlya vrachey. Moscow: Meditsina; 1999. (In Russ).
  12. Milovanov AP, Kirichenko AK. Tsitotrofoblasticheskaya invaziya – klyuchevoy mekhanizm razvitiya normal’noy i oslozhnennoy beremennosti. Krasnoyarsk: Litera-print, 2009. (In Russ).
  13. Kuznetsov RA, Peretyatko LP. Morphological and functional features of the extravillous cytotrophoblast and its microenvironment at the early spontaneous abortion. The Progressive Researches Science & Genesis. 2015;(1):29-33. (In Russ).
  14. Peretyatko LP, Fetisova IN, Golubeva AL, et al. Morphological changes in endometrium and chorions villous at the not developing pregnancy. Morphological Newsletter. 2009;(1-2):140-2. (In Russ).

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Copyright (c) 2021 Peretyatko L.P., Fetisova I.N., Fateeva N.V., Kuznetsov R.A., Fetisov N.S.



Свидетельство о регистрации СМИ ПИ № ФС77-76803 от 24 сентября 2019 года выдано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).


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