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Pathomorphology of gravid endometrium in patients with recurrent miscarriage in early periods with underlying chronic endometritis and the presence of low-functional alleles in folate cycle genes
- Authors: Peretyatko L.P.1, Fetisova I.N.1,2, Fateeva N.V.1, Kuznetsov R.A.1, Fetisov N.S.1
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Affiliations:
- V.N. Gorodkov Ivanovo Research Institute of Maternity and Childhood
- Ivanovo State Medical Academy
- Issue: Vol 29, No 2 (2021)
- Pages: 287-292
- Section: Original study
- URL: https://journals.eco-vector.com/pavlovj/article/view/34631
- DOI: https://doi.org/10.17816/PAVLOVJ34631
- ID: 34631
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Abstract
AIM: This study aimed to identify the peculiarities of pathomorphological alterations in the gravid endometrium and polymorphism of the folate cycle genes in female patients with recurrent early miscarriage and underlying chronic endometritis.
MATERIALS AND METHODS: The uterine mucosa of women who had uncomplicated pregnancies and decided to have an artifactual abortion within 5–12 weeks (control group, n = 36) and patients with recurrent miscarriage with underlying chronic endometritis (main group, n = 83) was histologically examined. The polymorphism of the folate cycle genes MTHFR С677Т (rs 1801133), MTHFR A1298C (rs 1801131), and MTRR A66G (rs 1801394) was studied in the female patients of the main group and women with the preserved reproductive function (comparison group, n = 52) through real-time polymerase chain reaction (PCR).
RESULTS: Fibroblast-like cells (FLCs) of the stroma sequentially differentiated into predecidual cells from 5 weeks to 12 weeks of uncomplicated pregnancy. In the case of recurrent miscarriage against background chronic endometritis, defect was observed in the differentiation of FLCs of the endometrial stroma. This finding was associated with a decrease in the morphometric parameters of the cells and an increase in the frequency of the occurrence of the low-functional allele 677T in the methylenetetrahydrofolate reductase gene.
CONCLUSION: The course and outcome of pregnancy in the first trimester were negatively affected by the structural inadequacy of endometrial stromal cells in recurrent miscarriage and chronic endometritis combined with the presence of the MTHFR 677T allele in a woman’s genotype.
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About the authors
Lybov P. Peretyatko
V.N. Gorodkov Ivanovo Research Institute of Maternity and Childhood
Email: peretiatko2011@yandex.ru
ORCID iD: 0000-0003-1047-6312
SPIN-code: 2795-9185
ResearcherId: В-1176-2017
MD, Dr.Sci.(Med.), Professor, Honored Doctor of the Russian Federation, Сhief Researcher of the Pathomorphology and Electron Microscopy Laboratory
Russian Federation, IvanovoIrina N. Fetisova
V.N. Gorodkov Ivanovo Research Institute of Maternity and Childhood; Ivanovo State Medical Academy
Email: ivgenlab@gmail.com
ORCID iD: 0000-0002-5769-1645
SPIN-code: 5995-0391
ResearcherId: B-1288-2017
MD, Dr.Sci.(Med.), Associate Professor, Leading Researcher of the Clinical Biochemistry and Medical Genetics Laboratory
Russian Federation, Ivanovo; IvanovoNatalya V. Fateeva
V.N. Gorodkov Ivanovo Research Institute of Maternity and Childhood
Author for correspondence.
Email: nata6120@bk.ru
ORCID iD: 0000-0002-4077-3310
SPIN-code: 5817-1380
ResearcherId: А-9999-2017
Junior Researcher of the Pathomorphology and Electron Microscopy Laboratory
Russian Federation, IvanovoRoman A. Kuznetsov
V.N. Gorodkov Ivanovo Research Institute of Maternity and Childhood
Email: rakuzz@mail.ru
ORCID iD: 0000-0001-6061-2955
SPIN-code: 1285-4151
ResearcherId: B-1106-2017
MD, Cand.Sci.(Med.), Junior Researcher of the Pathomorphology and Electron Microscopy Laboratory
Russian Federation, IvanovoNikolay S. Fetisov
V.N. Gorodkov Ivanovo Research Institute of Maternity and Childhood
Email: ivgenlab@gmail.com
ORCID iD: 0000-0002-6455-477X
SPIN-code: 5191-3751
ResearcherId: E-3831-2017
MD, Cand.Sci.(Med.), Junior Researcher of the Clinical Biochemistry and Medical Genetics Laboratory
Russian Federation, IvanovoReferences
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