Parameters of macrophage reaction and humoral immunity in patients with implant-associated in-flammation after primary endoprosthetics of knee joint

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Abstract

Aim. To study peculiarities of macrophage reaction and humoral immunity in patients with signs of implant-associated inflammation after the primary endoprosthetics of the knee joint.

Materials and Methods. Examination of 48 patients with signs of implant-associated inflammation after the primary endoprosthetics of the knee joints (the main group) and of 44 patients without signs of inflammatory reactions (comparison group) was carried out. Control group involved 30 healthy donors without diseases of the musculoskeletal system.

Results. In 1 and 12 months after the surgery, patients of the main group showed elevated levels of the factor inhibiting migration of macrophages (MIF), of macrophage stimulating protein (MSP), class A immunoglobulins (IgA), С3 and С4 complement components as compared to control, and also to each previous period. In 1 month after the surgery, the content of class M and G immunoglobulins (IgM and IgG) decreased relative to control; in 12 months after the surgery a more pronounced reduction was noted. Increase in serum concentrations of MIF, MSP, IgA, С3 and С4-complement components was noted in the comparison group in 1 and 12 months and also in comparison with the previous period. Reduction of the levels of IgM, IgG in 1 and 12 months after the operation was noted in comparison with control and with the previous period.

Conclusion. A study of parameters of macrophage reaction, proteins of complement system and the main classes of immunoglobulins permits to identify signs of implant-associated inflammation in 1 and 12 months after surgery, and, consequently, to determine patients of risk groups that require additional diagnostics measures.

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About the authors

Elena A. Galashina

Scientific Research Institute of Traumatology, Orthopedics and Neurosurgery Razumovsky Saratov State Medical University

Author for correspondence.
Email: koniuchienko1983@mail.ru
ORCID iD: 0000-0001-6209-9120
SPIN-code: 4016-0672
Scopus Author ID: 57190286406
ResearcherId: V-9086-2017
https://www.sarniito.com/

PhD in Biological Sciences, Junior Researcher of the
Department of Fundamental, Clinical and Experimental Researches

Russian Federation, Saratov

Ekaterina V. Gladkova

Scientific Research Institute of Traumatology, Orthopedics and Neurosurgery Razumovsky Saratov State Medical University

Email: gladkowa.katya@yandex.ru
ORCID iD: 0000-0002-6207-2275
SPIN-code: 3837-3244
Scopus Author ID: 36722610700
ResearcherId: V-9077-2017
https://www.sarniito.com/

PhD in Biological Sciences, Head of the Department of Fundamental, Clinical and Experimental Researches

Russian Federation, Saratov

Veronika V. Blinnikova

Scientific Research Institute of Traumatology, Orthopedics and Neurosurgery Razumovsky Saratov State Medical University

Email: bib@sarniito.com

MD, PhD, Doctor of the Clinical Laboratory Diagnostics Department

Russian Federation, Saratov

Sergey P. Shpinyak

Scientific Research Institute of Traumatology, Orthopedics and Neurosurgery Razumovsky Saratov State Medical University

Email: sergos83@rambler.ru
ORCID iD: 0000-0002-0345-6287
SPIN-code: 6540-1764
Scopus Author ID: 57191473935
ResearcherId: A-8021-2018

MD, PhD, Junior Researcher of the Innovative Projects for Traumatology and Orthopedics Department

Russian Federation, Saratov

Vladimir Y. Ulyanov

Scientific Research Institute of Traumatology, Orthopedics and Neurosurgery Razumovsky Saratov State Medical University

Email: v.u.ulyanov@gmail.com
ORCID iD: 0000-0002-9466-8348
SPIN-code: 8280-3339
Scopus Author ID: 56527335300
ResearcherId: A-4073-2014
https://www.sarniito.com/

MD, PhD, Deputy Director for Science and Innovations, Scientific Research Institute of Traumatology

Russian Federation, Saratov

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