Development of Methods to Obtain Grafts for the Anterior Abdominal Wall in Surgical Treatment of External Abdominal Hernias
- 作者: Magomedrasulova A.A.1, Chernykh A.V.1, Shevtsov A.N.1, Aydiyev G.A.1, Leibovich B.E.2, Pul'ver A.Y.3, Pul'ver N.A.1, Cherednikov E.F.1
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隶属关系:
- N. N. Burdenko Voronezh State Medical University
- RR-Medicine
- Institute of Biology of Aging
- 期: 卷 31, 编号 4 (2023)
- 页面: 513-524
- 栏目: Original study
- ##submission.dateSubmitted##: 20.04.2023
- ##submission.dateAccepted##: 13.07.2023
- ##submission.datePublished##: 22.11.2023
- URL: https://journals.eco-vector.com/pavlovj/article/view/340861
- DOI: https://doi.org/10.17816/PAVLOVJ340861
- ID: 340861
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INTRODUCTION: Currently, tissue engineering is one of the most promising fields of medicine dealing with search for solutions to the problems of creation of biocompatible structures capable of partial or complete replacement of organs or tissues of an organism. An advanced method of tissue engineering implies using an extracellular matrix (ECM) cleared from the cellular material. The main problem of obtaining an ECM consists in the difficulty of selecting a suitable decellularization method and exposure time.
AIM: Development of a method for obtaining grafts for the anterior abdominal wall (AAW) and evaluation of the histological properties of the ECM to determine the optimal protocol for donor tissue decellularization providing a complete removal of cellular material without any significant damage to the scaffold.
MATERIALS AND METHODS: The experiments were conducted on 10 White Flemish Giant male rabbits. All the animals were withdrawn from the experiment, after which, on autopsy, AAW grafts were obtained for evaluation of the decellularization properties of three detergents (alkaline solutions): 2% SDS (sodium dodecyl sulfate) solution, 2% CHAPS (zwitterionic surfactant, 3-[(3-cholamidopropyl)-dimethylammonio]-2-hydroxy-1-propansulfonate) solution, and 1% Triton-X 100 solution. The decellularization quality was controlled in histological examination.
RESULTS: Morphological evaluation of tissue samples revealed that after decellularization of the material with 2% SDS solution and 2% CHAPS solution, the number of cells remaining in the preparations was comparable and significantly less (Me = 10 in a microscope field) than after treatment with 1% Triton-X 100 solution (Me = 45 in a microscope field). However, decellularization with 2% CHAPS solution led to fiber ruptures and tissue edema manifested by an increase in the distance between the fibers (Me = 163 µm).
CONCLUSIONS: Decellularization of the anterior abdominal wall tissues with 2% SDS solution provides a sufficient cell elimination while preserving the fiber integrity and ECM structure. 2% CHAPS solution was comparable to 2% SDS solution in terms of the quality of cell elements removal, but its more aggressive effect on collagen fibers was noted (their multiple ruptures and a more pronounced edema). 1% Triton-X 100 solution appeared to be of low efficiency in elimination of cellular material, although it had a minimal effect on the integrity of ECM network.
全文:
作者简介
Asiyat Magomedrasulova
N. N. Burdenko Voronezh State Medical University
Email: asiyat15062015@mail.ru
ORCID iD: 0000-0002-3158-1480
SPIN 代码: 7263-2267
Assistant of the Department of Operative Surgery with Topographic Anatomy
俄罗斯联邦, VoronezhAleksandr Chernykh
N. N. Burdenko Voronezh State Medical University
Email: chernuch@vrngmu.ru
ORCID iD: 0000-0002-6281-0020
SPIN 代码: 8444-7010
MD, Dr. Sci. (Med.), Professor
俄罗斯联邦, VoronezhArtem Shevtsov
N. N. Burdenko Voronezh State Medical University
Email: shan-87@yandex.ru
ORCID iD: 0000-0001-8641-2847
SPIN 代码: 5647-9491
MD, Cand. Sci. (Med.)
俄罗斯联邦, VoronezhGamzat Aydiyev
N. N. Burdenko Voronezh State Medical University
Email: gamzat200000@gmail.com
ORCID iD: 0000-0003-0966-6637
SPIN 代码: 4004-5565
Student
俄罗斯联邦, VoronezhBoris Leibovich
RR-Medicine
Email: bel.46@mail.ru
ORCID iD: 0000-0003-2259-7656
SPIN 代码: 4206-4245
Head of the Pathology Department
俄罗斯联邦, VoronezhAleksandr Pul'ver
Institute of Biology of Aging
Email: pulver.ibs@gmail.com
ORCID iD: 0000-0001-6673-1859
General Manager
俄罗斯联邦, VoronezhNatal'ya Pul'ver
N. N. Burdenko Voronezh State Medical University
Email: Natalya_pulver@mail.ru
ORCID iD: 0000-0003-4549-5476
SPIN 代码: 6226-0654
MD, Cand. Sci. (Med.)
俄罗斯联邦, VoronezhEvgeniy Cherednikov
N. N. Burdenko Voronezh State Medical University
编辑信件的主要联系方式.
Email: facult-surg.vsmuburdenko@yandex.ru
ORCID iD: 0000-0001-7521-0211
SPIN 代码: 7683-5973
MD, Dr. Sci. (Med.), Professor
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