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Possibilities of proteolytic enzymes in the treatment of some forms of male infertility
- Authors: Neymark A.I.1, Neymark B.A.1, Nozdrachev N.A.1, Davydov A.V.1, Kablova I.V.1, Yakovets Y.V.1, Levin V.P.1, Plotnikova A.Y.1
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Affiliations:
- Altai State Medical University of the Ministry of Healthcare of the Russian Federation
- Issue: Vol 10, No 3 (2020)
- Pages: 217-221
- Section: Original articles
- URL: https://journals.eco-vector.com/uroved/article/view/35132
- DOI: https://doi.org/10.17816/uroved35132
- ID: 35132
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Abstract
In the study 46 men with impaired fertility and the presence of viscosipathy were included. The patients’ age ranged from 22 to 45 years, the average duration of infertility in marriage was 3.5 ± 2.4 years. All patients with Longidaza drugs according to the following scheme were treated. First, ten intramuscular injections of Longidaza® lyophilisate were performed to prepare a solution of 3000 IU once every five days, then Longidaza® suppositories 3000 IU rectally once every three days were prescribed, for a total of 15 suppositories. The total duration of the course of treatment is 90 days. A decrease in sperm viscosity from 2.1 ± 0.05 cm to 0.6 ± 0.07 cm (p < 0.05), an increase in total sperm motility (from 32.6 ± 2.9 to 61 ± 3.1%, p < 0.05) and the number of sperm with progressive movement (from 22 ± 1.1 to 36.7 ± 2.9 %, p < 0.05) after the treatment were revealed. Also, in the course of treatment, the restoration of the antioxidant protection of the ejaculate was noted, as evidenced by an increase in the activity of glutathione peroxidase and superoxide dismutase and a decrease in the content of malon dialdehyde. During the first year after the end of treatment, conception in marriage was registered in 21 (45.7%) patients. The results of the study indicate that a violation of the lipid peroxidation system can lead to both an increase in sperm viscosity and a disruption in the activity of acrosomal enzymes, as a result of which it becomes difficult for spermatozoa to penetrate the ovum. Longidaza is able to increase the hyaluronidase activity of the sperm acrosome, thereby improving the fertilizing ability of the ejaculate.
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About the authors
Aleksandr I. Neymark
Altai State Medical University of the Ministry of Healthcare of the Russian Federation
Email: neimark.a@mail.ru
ORCID iD: 0000-0002-5741-6408
Scopus Author ID: 7102411541
https://asmu.ru/ob-universitete/fakultety/lechebnyy-fakultet/spetsializirovannoy-khirurgii-po-urologii-travmatologii-i-oftalmologii/#
Doctor of Medical Science, Professor, Head of the Department of Urology and Andrology with Course of Additional Professional Education
Russian Federation, BarnaulBoris A. Neymark
Altai State Medical University of the Ministry of Healthcare of the Russian Federation
Email: neimark.b@mail.ru
ORCID iD: 0000-0001-8009-3777
https://asmu.ru/ob-universitete/fakultety/lechebnyy-fakultet/spetsializirovannoy-khirurgii-po-urologii-travmatologii-i-oftalmologii/#
Doctor of Medical Science, Professor of the Department of Urology and Andrology with Course of Additional Professional Education
Russian Federation, BarnaulNikolay A. Nozdrachev
Altai State Medical University of the Ministry of Healthcare of the Russian Federation
Email: nozdrachevuro@mail.ru
ORCID iD: 0000-0001-9203-3499
Candidate of Medical Science, Associated Professor of the Department of Urology and Andrology with Course of Additional Professional Education
Russian Federation, BarnaulAndrej V. Davydov
Altai State Medical University of the Ministry of Healthcare of the Russian Federation
Email: andre1763@mail.ru
ORCID iD: 0000-0001-8212-2623
Doctor of Medical Science, Professor of the Department of Urology and Andrology with Course of Additional Professional Education
Russian Federation, BarnaulIrina V. Kablova
Altai State Medical University of the Ministry of Healthcare of the Russian Federation
Author for correspondence.
Email: irina_kablova@mail.ru
ORCID iD: 0000-0002-3450-3875
Candidate of Medical Science, Associated Professor of the Department of Urology and Andrology with Course of Additional Professional Education
Russian Federation, BarnaulYaroslava V. Yakovets
Altai State Medical University of the Ministry of Healthcare of the Russian Federation
Email: yyakovec@mail.ru
ORCID iD: 0000-0001-5974-6534
Candidate of Medical Science, Associated Professor of the Department of Urology and Andrology with Course of Additional Professional Education
Russian Federation, BarnaulVladimir P. Levin
Altai State Medical University of the Ministry of Healthcare of the Russian Federation
Email: urologagmu@mail.ru
ORCID iD: 0000-0001-9203-3499
Candidate of Medical Science, Assistant Professor of the Department of Urology and Andrology with Course of Additional Professional Education
Russian Federation, BarnaulAnzhelika Yu. Plotnikova
Altai State Medical University of the Ministry of Healthcare of the Russian Federation
Email: urologagmu@mail.ru
student
Russian Federation, BarnaulReferences
- Божедомов В.А., Теодорович О.В. Эпидемиология и причины аутоиммунного мужского бесплодия // Урология. – 2005. – № 1. – С. 35–44. [Bozhedomov VA, Teodorovich OV. Epidemiology and causes of autoimmune male infertility. Urologiia. 2005;(1):35-44. (In Russ.)]
- Rowe PJ, Comhaire FH, Hargreave TB, Mellows HJ. WHO Manual for the Standardized Investigation, Diagnosis and Management of the Infertile Male. Cambridge University Press; 2000.
- Тер-Аванесов Г.В. Андрологические аспекты бесплодного брака. Практическое руководство. – М., 2000. – 48 с. [Ter-Avanesov GV. Andrologicheskie aspekty besplodnogo braka. Prakticheskoe rukovodstvo. Moscow; 2000. 48 p. (In Russ.)]
- Калинина С.Н., Кореньков Д.Г., Фесенко В.Н. Лечение сперматологических нарушений и оксидативного стресса после перенесенных репродуктивно значимых заболеваний, вызванных инфекциями, передающимися половым путем // Урологические ведомости. – 2018. – Т. 8. – № 4. – C. 5–15. [Kalinina SN, Koren’kov DG, Fesenko VN. Treatment of spermatologic disorders and oxidative stress after reproductively significant diseases caused by sexually transmitted infection. Urologicheskie vedomosti. 2018;8(4):5-15. (In Russ.)]. https://doi.org/10.17816/uroved845-15.
- Agarwal A, Said TM. Oxidative stress, DNA damage and apoptosis in male infertility: a clinical approach. BJU Int. 2005;95(4): 503-507. https://doi.org/10.1111/j.1464-410X.2005.05328.x.
- Tremellen K, Miari G, Froiland D, Thompson J. A randomised control trial examining the effect of an antioxidant (Menevit) on pregnancy outcome during IVF-ICSI treatment. Aust N Z J Obstet Gynaecol. 2007;47(3):216-221. https://doi.org/10.1111/j.1479-828X.2007.00723.x.
- Гамидов С.И., Иремашвили В.В., Тхагопсоева П.А. Терапия нарушения фертильности у мужчин: перспективные результаты европейских исследований // Эффективная фармакотерапия. – 2009. – № 26. – С. 26–31. [Gamidov SI, Iremashvili VV, Tkhagopsoeva PA. Terapiya narusheniya fertil’nosti u muzhchin: perspektivnye rezul’taty evropeyskikh issledovaniy. Effektivnaya farmakoterapiya. 2009;(26):26-31. (In Russ.)]
- Aitken RJ, Gordon E, Harkiss D, et al. Relative impact of oxidative stress on the functional competence and genomic integrity of human spermatozoa. Biol Reprod. 1998;59(5):1037-1046. https://doi.org/10.1095/biolreprod59.5.1037.
- De Lamirande E, Gagnon C. The dark and bright sides of reactive oxygen species on sperm function. In: The male gamete: from basic science to clinical application. Ed. by C. Gagnon. Vienna: Cache River Press; 1999. P. 455-467.
- Алоян К.А., Матвеев А.В., Морев В.В., Корнеев И.А. Физиологические механизмы обеспечения подвижности сперматозоидов // Урологические ведомости. – 2013. – Т. 3. – № 4. – C. 14–19. [Aloyan KA, Matveev AV, Morev VV, Korneev IA. Physiology of sperm motility. Urologicheskie vedomosti. 2013;3(4):14-19. (In Russ.)]. https://doi.org/10.17816/uroved 3414-19.
- Громенко Д.С. Применение наукоемких технологий для оценки фертильности мужчин // Вестник новых медицинских технологий. – 2008. – Т. 15. – № 4. – С. 118–120. [Gromenko DS. The use of scientific technologies for estimation of man’s reproductive function. Journal of new medical technologies. 2008;15(4):118-120. (In Russ.)]
- Kao SH, Chao HT, Chen HW, et al. Increase of oxidative stress in human sperm with lower motility. Fertil Steril. 2008;89(5): 1183-1190. https://doi.org/10.1016/j.fertnstert.2007.05.029.
- Kemal Duru N, Morshedi M, Oehninger S. Effects of hydrogen peroxide on DNA and plasma membrane integrity of human spermatozoa. Fertil Steril. 2000;74(6):1200-1207. https://doi.org/10.1016/s0015-0282(00)01591-0.
- Loft S, Kold-Jensen T, Hjollund NH, et al. Oxidative DNA damage in human sperm influences time to pregnancy. Hum Reprod. 2003;18(6):1265-1272. https://doi.org/10.1093/humrep/deg202.
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