The effect of lithium chloride on the activation of neutrophils by the action of bacterial components in vitro

Activation of neutrophils is a mandatory stage and a sensitive marker of systemic inflammatory conditions that can lead to the development of multiple organ failure. The purpose of the study was to study the anti-inflammatory effect of lithium chloride on human neutrophils in vitro. The study was carried out on 5 healthy donors isolated from blood neutrophils, some of which were activated using 100 mkMfMLP and 100 ng/ml lipopolysaccharide, then their activity was evaluated using fluorescent antibodies to CD11b and CD66b degranulation markers. Intact and activated neutrophils were treated with a solution of lithium chloride at a concentration of 9 mmol. Lithium chloride reduces the level of neutrophil activation, having an anti-inflammatory effect on neutrophils preactivated by bacterial components, and tends to decrease the expression level of CD11b and CD66b molecules on the surface of intact neutrophils, inhibiting their activation and degranulation.

CD11b, CD66b, CD11b, CD66b, lithium chloride, neutrophils, CD11b, CD66b, anti-inflammatory effect