ABNORMAL HYPERMETHYLATION OF HOXA10 AND HOXA11 GENES IN CHRONIC ENDOMETRITIS-RELATED INFERTILITY


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Abstract

The HOXA10 and HOXA11 genes and their encoded protein products are now considered to be one of the key regulators of endometrial receptivity during embryo implantation, which determine fertility as a whole. It is known that patients with endometriosis-related infertility exhibit lower levels of HOXA and HOXA11 proteins in endometrial tissue because of hypermethylation within the promoter regions encoding these protein genes. Objective. To investigate the methylation in the promoter regions of the HOXA10 and HIXA11 genes in the endometrium of reproductive-aged women with infertility in the presence of chronic endometritis. Subject and methods. The bisulfite sequencing method was used to study the level of promoter methylation of the HOXA10 and HOXA11 genes in 25 eutopic endometrial biopsy specimens obtained from reproductive-aged women with infertility in the presence of chronic endometritis. Results. It was established that hypermethylation of the HOXA11 and HOXA10 genes was observed in 64 and 84% of the biopsy specimens, respectively. At the same time, promoter methylation of the HOXA10 gene correlated with the duration of infertility. Conclusion. The findings agree well with the data previously obtained by other authors and allow the methylation of the HOXA10 and HOXA11 genes to be considered as a potential molecular marker for infertility associated with gynecological diseases.

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About the authors

Gennady T. Sukhikh

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: g_sukhikh@oparina4.ru
MD, professor, academician of the RAS, Director Moscow 117997, Ac. Oparina str. 4, Russia

Andrew I. Osipyants

Dmitry Rogachev Federal Research-and-Clinical Center for Pediatric Hematology, Oncology, and Immunology

Email: osssip@gmail.com
researcher at the Laboratory of biochemistry and enzymology Moscow 117997, GSP-7, Samora Machel str. 1, Russia

Larisa Ivanovna Maltseva

Kazan State Medical Academy, Ministry of Health of Russia

Email: laramalc@mail.ru
MD, Professor, Head of the Department of Obstetrics and Gynecology Kazan 420011, Butlerova str. 36, Russia

Gulnara R. Smolina

Kazan State Medical Academy, Ministry of Health of Russia

Email: smolinagulnara@mail.ru
Competitor of the Department of Obstetrics and Gynecology Kazan 420011, Butlerova str. 36, Russia

Andrey Aleksandrovich Poloznikov

Dmitry Rogachev Federal Research-and-Clinical Center for Pediatric Hematology, Oncology, and Immunology

Email: andrey.poloznikov@gmail.com
PhD, Head. Laboratory of biochemistry and enzymology Moscow 117997, GSP-7, Samora Machel str. 1, Russia

Ekaterina Leonidovna Muyzhnek

MiraxBioPharma

Email: MuyzhnekEL@ilmixgroup.ru
PhD, Scientific Director Moscow 121248, Kutuzovsky Prospekt. 12 bld. 2, Russia

Vsevolod I. Kiselev

Institute of Biomedical Problems, Peoples' Friendship University of Russia

Email: vkis10@mail.ru
Ph.D., professor, corresponding member, RAS, deputy director Moscow 115093, Russia, Moscow, Podolsk sh. 8 bld. 5

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